New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms

New rapid, sensitive, and accurate kinetic spectrophotometric methods were developed, for the first time, to determine omeprazole (OMZ) in its dosage forms. The methods were based on the formation of charge-transfer complexes with both iodine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The variables that affected the reactions were carefully studied and optimized. The formed complexes and the site of interaction were examined by UV/VIS, IR, and 1H-NMR techniques, and computational molecular modeling. Under optimum conditions, the stoichiometry of the reactions between OMZ and the acceptors was found to be 1 : 1. The order of the reactions and the specific rate constants were determined. The thermodynamics of the complexes were computed and the mechanism of the reactions was postulated. The initial rate and fixed time methods were utilized for the determination of OMZ concentrations. The linear ranges for the proposed methods were 0.10–3.00 and 0.50–25.00 μg mL−1 with the lowest LOD of 0.03 and 0.14 μg mL−1 for iodine and DDQ, respectively. Analytical performance of the methods was statistically validated; RSD was <1.25% for the precision and <1.95% for the accuracy. The proposed methods were successfully applied to the analysis of OMZ in its dosage forms; the recovery was 98.91–100.32% ± 0.94–1.84, and was found to be comparable with that of reference method

Performance Evaluation of Different Universal Steganalysis Techniques in JPG Files

Steganalysis is the art of detecting the presence of hidden data in files. In the last few years, there have been a lot of methods provided for steganalysis. Each method gives a good result depending on the hiding method. This paper aims at the evaluation of five universal steganalysis techniques which are “Wavelet based steganalysis”, “Feature Based Steganalysis”, “Moments of characteristic function using wavelet decomposition based steganalysis”, “Empirical Transition Matrix in DCT Domain based steganalysis”, and “Statistical Moment using jpeg2D array and 2D characteristic function”. A large Dataset of Images -1000 images- are subjected to three types of steganographic techniques which are “Outguess”, “F5” and “Model Based” with the embedding rate of 0.05, 0.1, and 0.2. It was followed by extracting the steganalysis feature used by each steganalysis technique for the stego images as well as the cover image. Then half of the images are devoted to train the classifier. The Support vector machine with a linear kernel is used in this study. The trained classifier is then used to test the other half of images, and the reading is reported The “Empirical Transition Matrix in DCT Domain based steganalysis” achieves the highest values among all the properties measured and it becomes the first choice for the universal steganalysis technique

ADSORPTION OF FOOD COLORING ALLURA RED DYE (E129) FROM AQUEOUS SOLUTIONS USING ACTIVATED CARBON

The adsorption behavior of Allura red (E129) from aqueous solutions onto activated carbon was successfully investigated. All factors affecting the adsorption process were carefully studied and the conditions were optimized. Adsorption of E129 onto activated carbon was found to increase by decreasing the mass of activated carbon, pH and ionic strength of the solution and by increasing temperature. The adsorption capacity of the activated carbon for Allura red was relatively high. Under the optimum conditions, the maximum adsorption capacity for E129 dye was 72.85 mg/g. Three adsorption models; Langmuir, Freundlich and Temkin model were investigated regarding the adsorption of E129. The models’ parameters KL, qm, R2, (n) were determined and found to be 0.0222, 72.85 mg/g, 0.9057-0.9984, and 0.992, respectively. Also, pseudo first and second-order kinetic models were tested to determine the best-fit model to the adsorption of E129 dye onto activated carbon. The results showed that the adsorption of E129 onto activated carbon obeyed both the Freundlich isotherm and pseudo second-order kinetic models. Moreover, thermodynamic studies indicated that the adsorption of E129 dye onto the activated carbon was spontaneous.Â

Novel Analytical Study For The Charge-Transfer Reactions Of Omeprazole With 2,3-Dichloro-Naphthoquinone And 2,3,5,6-Tetrabromo- 1,4-Benzoquinone: Application For The Development Of Microwell Assay Of Omeprazole

Novel analytical study was performed in order to develop and validate new high-throughput microwell-based spectrophotometric assays for determination of omeprazole (OMZ) in its pharmaceutical formulations. The proposed assays were based on the charge-transfer (CT) reaction of OMZ with 2,3-dichloronaphthoquinone (DCNQ) and 2,3,5,6-tetrabromo-1,4-benzo-quinone (BROM). In the present study, the CT reactions was carried out in microwell plates as reaction vessels in order to increase the automation of the assays and the efficiency of its use in quality control laboratories (QCLs). All factors affecting the CT reactions were carefully studied, and the conditions were optimized. Kinetics and stoichiometry of the CT reactions were investigated, and the mechanism was postulated. Activation energy of the CT reactions was determined and found to be 13.87 and 16.27 Kcal mol−1 for the reaction of OMZ with DCNQ and BROM, respectively. The initial rate and fixed time methods were applied for generating the calibration graphs for determination of OMZ concentrations. Under the optimum conditions, the linear range was 0.145 – 1.45 x 10-4 and 1.45 – 7.25 x 10-4 M with LOD of 0.6 and 6.0 microgram ml-1 for DCNQ and BROM, respectively. Analytical performance of the proposed methods, in terms of accuracy and precision, was statistically validated and the results were satisfactory; RSD was &lt;2.8% for both repeatability and reproducibility. The proposed methods were successfully applied to the analysis of OMZ in its dosage forms and the recovery results (98.64 – 100.6 ± 0.25 -2.74 %) were comparable with those of the reported method. The developed method may provide a safer and economic tool for the analysis of OMZ in QCLs

Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4-sulphonate

New selective and sensitive spectrophotometric and spectrofluorometric methods have been developed and validated for the determination of amantadine hydrochloride (AMD) in capsules and plasma. The methods were based on the condensation of AMD with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product. The spectrophotometric method involved the measurement of the colored product at 460  nm. The spectrofluorometric method involved the reduction of the product with potassium borohydride, and the subsequent measurement of the formed fluorescent reduced AMD-NQS product at 382  nm after excitation at 293  nm. The variables that affected the reaction were carefully studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9972–0.9974) and low LOD (1.39 and 0.013 μg mL−1) were obtained in the ranges of 5–80 and 0.05–10  μg mL−1 for the spectrophotometric and spectrofluorometric methods, respectively. The precisions of the methods were satisfactory; RSD ≤2.04%. Both methods were successfully applied to the determination of AMD in capsules. As its higher sensitivity, the spectrofluorometric method was applied to the determination of AMD in plasma; the recovery was 96.3–101.2 ± 0.57–4.2%. The results obtained by the proposed methods were comparable with those obtained by the official metho

Effect of diet on the quality and quantity of venom produced by Apis cerana

The bee venom samples from honey bee (Apis cerana) foraged on seven plants dominating seven sites in Malaysia were collected. The protein content of the pollen loads of these test plants were tested according to the method of Kjeldahl (AOAC 1990). The samples were analysed to determine the effects of diet type on the quantity and quality of its constituents – melittin, phospholipase A2 and apamin using HPLC. The highest melittin (677.86μg/ml), phospholipase (477.96 μg/ml) and apamin (136.10 μg/ml) content were recorded in venom collected from honey bee colonies foraged on Durian plant which has protein content (31.7%) of its pollen loads. A positive correlation was recorded between the protein content with melittin (R2= 0.9372), phospholipase A2 (R2= 0.7208) and apamin (R2=0.4128), while a negative correlation was observed between the protein content and the weight of the venom mount produced (R2= -0.7549). A direct relationship was observed between the quality of the venom and the protein content of pollen loads, while the quantity of the venom was not in accordance with the venom quality

Phytochemical composition and antimicrobial properties of Markhamia platycalyx (Baker) Sprague leaf

Purpose: To isolate new antimicrobial agents from the leaves of Markhamia platycalyx (Baker) Sprague and assess their phytochemical characteristics and antimicrobial activity. Methods: Different chromatographic and spectroscopic techniques (NMR and ESI-MS) were applied for the identification of antimicrobial compounds. Agar-well diffusion technique was used for determination of antimicrobial activity. Anti-HCV effects were investigated using VITROS Anti-HCV assay. Results: Eighteen compounds were isolated for the first time from this genus. These were phytol, noctacosanoic acid (OCTA), tormentic acid and β-sitosterol-3-O-(6'-O-heptadecanoyl)-β-Dglucopyranoside. The other compounds were β-sitosterol, ursolic acid (URSA), oleanolic acids, pomolic acid (POMA), 2-epi-tormentic and β-sitosterol-3-O-β-D-glucopyranoside. However, stigmasterol and acteoside, which were seen in previous studies, were also present. Total ethanol extract (TEE) was the most effective against Escherichia coli, with the lowest minimum inhibitory concentration (MIC) of 1.0 µg/mL. Acteoside, URSA and 2-epi-tormentic acid showed the highest antibacterial effect on Pseudomonas aeruginosa while 2-epi-tormentic acid and acteoside produced the least MIC on Candida glabrata. These effects were superior to those produced by standard antibiotics. However, 2-epitormentic acid and β-sitosterol-3-O-β-D-glucopyranoside had no anti-HCV effects. Conclusion: Due to the good antimicrobial properties of Markhamia platycalyx, it is a potential source of new antimicrobial drugs

Sunflower oil-based hyperbranched alkyd/spherical ZnO nanocomposite modeling for mechanical and anticorrosive applications

Approaches for designing advanced nanomaterials with hyperbranched architectures and lack of volatile organic content (VOC) have attracted considerable attention. In this study, eco-friendly hyperbranched alkyd resins for mechanical and anticorrosive coatings with high solid content were successfully synthesized based on sunflower oil (SFO) via a polyesterification approach. These resins are characterized by energy-efficient polymer synthesis, lack of gelation properties, high functionality, and low viscosity. A chemical precipitation process was used to fabricate zinc oxide (ZnO) spherical nanostructures with controlled diameters and morphologies. A series of conformal, novel, low-cost SFO-based hyperbranched alkyd/spherical ZnO nanocomposites were fabricated through an ex situ method. Various nanofiller concentrations were distributed to establish synergetic effects on the micro-nano binary scale performance of the materials. The features of the nanocomposites, including the molecular weight, acid and hydroxyl values of the prepared alkyd resins, were concomitantly assessed through various standard tests. The nanocomposites were also subjected to various tests to determine their surface adhesion and mechanical properties, such as impact, T-bending, crosscut, and abrasion resistance tests. Furthermore, the physico-mechanical properties, anticorrosive behavior, thermal stabilities and cellular cytotoxicities of the fabricated materials were assessed. The anticorrosive features of the nanocomposites were investigated through salt spray tests in 5 wt% NaCl. The results indicate that well-dispersed ZnO nanospheres (0.5%) in the interior of the hyperbranched alkyd matrix improve the durability and anticorrosive attributes of the composites; thus, they exhibit potential applications in eco-friendly surface coatings

Sixth Generation (6G)Wireless Networks: Vision, Research Activities, Challenges and Potential Solutions

The standardization activities of the fifth generation communications are clearly over and deployment has commenced globally. To sustain the competitive edge of wireless networks, industrial and academia synergy have begun to conceptualize the next generation of wireless communication systems (namely, sixth generation, (6G)) aimed at laying the foundation for the stratification of the communication needs of the 2030s. In support of this vision, this study highlights the most promising lines of research from the recent literature in common directions for the 6G project. Its core contribution involves exploring the critical issues and key potential features of 6G communications, including: (i) vision and key features; (ii) challenges and potential solutions; and (iii) research activities. These controversial research topics were profoundly examined in relation to the motivation of their various sub-domains to achieve a precise, concrete, and concise conclusion. Thus, this article will contribute significantly to opening new horizons for future research direction