Comparing mannose binding lectin genetic diversity in intracellular and extracellular pathogens

One of the important immunological factors in diseases is mannose binding lectin (MBL). The aim of present study is to determine the distribution of the alleles of mannose-binding lectin gene codon 52,54, 57 and promoter variants H/L, X/Y, P and Q in confirmed VL patients as an intracellular pathogen while compares with extracellular pathogens (in renal infection) and seek correlation between thesevariants and intracellular and extracellular infections. Fifty eight confirmed VL patients’ blood samples were compared with fifty eight blood samples of patients received renal in results of renal infections.MBL genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism. Frequency of defective allele B in extracellular pathogens was more than intracellular pathogens (P = 0.0001), and in contrary prevalence of wild type allele A in intracellular pathogens was more than extracellular pathogens (P = 0.0001), and in other alleles and variants there was not any significant difference. In conclusion, there was more prevalence of alleles with low mannose binding lectin serum level in extrallelular pathogens which can be consider as a risk factor for these infections. In other hand prevalence of high concentration alleles in intracellular pathogens indicate the role of mannose binding lectin level for susceptibility to intracellular pathogens

Coenzyme Q10 in the treatment of heart failure: A systematic review of systematic reviews

Introduction: This article is an attempt to provide an overview of systematic reviews to determine the efficacy of CQ10 supplementation in the treatment of patients with cardiovascular diseases (CVD). Method and material: All reviews were identified through a systematic search of the following databases: Cochrane, DARE, Ovid, EMBASE, ISI Web of Knowledge, and PubMed. Check references studies and the quality of the studies was assessed by means of AMSTTAR. No meta-analyses were performed due to the heterogeneity of studies. Result: Extracted data for Seven systematic reviews for primary outcomes, net changes in cardiac output, cardiac index, New York Heart Association functional classification, improved survival, based on existing evidence, there is a case for use of CoQ10 as an adjunctive therapy in congestive heart failure, especially in those patients unable to tolerate mainstream medical therapies. Conclusion: Evidence suggests that the CoQ10 supplement may be a useful tool for managing patients with heart failure. © 201

Coenzyme Q10 in the treatment of heart failure: A systematic review of systematic reviews

Introduction: This article is an attempt to provide an overview of systematic reviews to determine the efficacy of CQ10 supplementation in the treatment of patients with CVD. Method and material: All reviews were identified through a systematic search of the following databases: Cochrane, DARE, Ovid, EMBASE, ISI Web of Knowledge, and PubMed. Check references studies and the quality of the studies was assessed by means of AMSTTAR. No meta-analyses were performed due to the heterogeneity of studies. Result: Extracted data for Seven systematic reviews for primary outcomes, net changes in cardiac output, cardiac index, New York Heart Association functional classification, improved survival, based on existing evidence, there is a case for use of CoQ10 as an adjunctive therapy in congestive heart failure, especially in those patients unable to tolerate mainstream medical therapies. Conclusion: Evidence suggests that the CoQ10 supplement may be a useful tool for managing patients with heart failure. © 2018

Interactive effects of Arbuscular mycorrhizal fungi and rhizobial strains on chickpea growth and nutrient content in plant

Legumes form a tripartite symbiosis with Arbuscular mycorrhizal fungi (AMF) and rhizobia. Chickpea plants were inoculated with six strains of Mesorhizobium ciceri and three AMF species, Glomus intraradices (GI), G. mosseae (GM) and G. etunicatum (GE). The plants inoculated with a number of AMF species and bacterial strains increased overall plant dry mass compared to non-inoculated plants. GE was the most efficient in increasing plant dry matter. Individual AMF species were more effective than when mixed (GI+GM+GE). Bacterial treatments had increasing effect on root colonization by GI, GM and GI+GM+GE. The results revealed that dual inoculation with AMF and rhizobium enhanced nitrogen, phosphorus, zinc, iron and copper content in plants but these increasing effects was different between fungal and bacterial treatments.Key words: Arbuscular mycorrhizal fungi, Mesorhizobium ciceri, nutrient content, root colonization, nodule, chickpea

Nitric oxide metabolite levels during the ectopic osteoinduction in rats

Abstract Nitric oxide (NO) is a cell-signaling molecule that has diverse biological functions. Recent evidence suggests that its production may regulate the metabolism of the osteoblasts and osteoclasts. The aim of this study was to evaluate levels of nitrite and nitrates (NO metabolites) during ectopic osteoinduction in rats. Eighteen male Sprague–Dawley rats (body weight 200–300 g) were used in this study. All animals were anesthetized and the right and left flank areas were shaved, and under aseptic conditions, a muscular pouch was created in each flank: the left was filled with 20 mg of demineralized bone matrix and the right remained empty (sham). Radiographs were taken at 2, 4, and 6 weeks after surgery to trace the ectopic bone formation and muscle mineralization. Blood samples were taken before (as baseline values) and at 2, 4, and 6 weeks after surgery. The mean values of NO metabolites after 6 weeks were significantly higher (p<0.05) than baseline data and at 2 weeks post-surgery. Results from this study indicate that the ectopic osteoinduction caused increased activity of the osteoblasts which subsequently caused increased serum levels of NO metabolites (nitrites and nitrates)

Mannose Binding Lectin (MBL) and Its Clinical Significance

ABSTRACT BACKGROUND AND OBJECTIVE: Mannose Binding Lectin (MBL) is a key molecule in innate immunity, thi

EW-7197 prevents ulcerative colitis-associated fibrosis and inflammation

EW‐7197 is a transforming growth factor‐β type I receptor kinase inhibitor with potential anti‐inflammatory and antifibrotic properties. Here, we investigate the potential therapeutic effects of EW‐7197 in a murine model of ulcerative colitis. EW‐7197 attenuated the colitis disease activity index by improving rectal bleeding, body weight, and degree of stool consistency. EW‐7197 also reduced colorectal tissue damage and the colon histopathological score by reducing crypt loss, mucosal damage, and tissue inflammation. Moreover, EW‐7197 appeared to ameliorate the inflammatory and fibrotic responses by reducing oxidative stress, reducing submucosal edema and inflammatory cell infiltration, downregulating proinflammatory and pro‐fibrotic genes, and inhibiting excessive collagen deposition in inflamed and fibrotic ulcerative colitis tissues. These results suggest that EW‐7197 has potentially useful therapeutic properties against colitis, with clinically translational potential of inhibiting key pathological responses of inflammation and fibrosis in patients with colitis

Enhanced electro kinetic- pseudo-Fenton degradation of pyrene-contaminated soil using Fe 3 O 4 magnetic nanoparticles: A data set

The aim of the data were to increase the treatment efficiency of pyrene from soil using Nano catalysts magnetite iron oxide (Fe 3 O 4 )and combined with electro kinetic. Soil provided with 100 mg/kg concentration and removal of pyrene done with EK-Fenton process. Nano catalyst was synthesized via a facile co-precipitation method and characterized by FTIR, XRD, SEM, EDX, VSM techniques. The effects of some operational parameters include catalyst dosage, pH, hydrogen peroxide concentration and the voltage were studied on the removal efficiency of pyrene. Results indicated the removal efficiency was obtained 87 under optimal conditions (pH = 3, Nano catalyst dosage= 1 g/l, H 2 O 2 =10 mM and voltage 30 V). Electrokinetic Fenton process can be as efficient and effective method for the removal of pyrene from contaminated soil using Nano Catalyst Fe 3 O 4 introduced in optimal conditions. © 201