4,642 research outputs found
C-terminal truncated hepatitis B virus X protein promotes hepatocellular carcinogenesis through induction of cancer and stem cell-like properties
published_or_final_versio
Atomic-scale combination of germanium-zinc nanofibers for structural and electrochemical evolution
Alloys are recently receiving considerable attention in the community of rechargeable batteries as possible alternatives to carbonaceous negative electrodes; however, challenges remain for the practical utilization of these materials. Herein, we report the synthesis of germanium-zinc alloy nanofibers through electrospinning and a subsequent calcination step. Evidenced by in situ transmission electron microscopy and electrochemical impedance spectroscopy characterizations, this one-dimensional design possesses unique structures. Both germanium and zinc atoms are homogenously distributed allowing for outstanding electronic conductivity and high available capacity for lithium storage. The as-prepared materials present high rate capability (capacity of similar to 50% at 20 C compared to that at 0.2 C-rate) and cycle retention (73% at 3.0 C-rate) with a retaining capacity of 546 mAh g(-1) even after 1000 cycles. When assembled in a full cell, high energy density can be maintained during 400 cycles, which indicates that the current material has the potential to be used in a large-scale energy storage system
Superluminal motion of a relativistic jet in the neutron star merger GW170817
The binary neutron star merger GW170817 was accompanied by radiation across
the electromagnetic spectrum and localized to the galaxy NGC 4993 at a distance
of 41+/-3 Mpc. The radio and X-ray afterglows of GW170817 exhibited delayed
onset, a gradual rise in the emission with time as t^0.8, a peak at about 150
days post-merger, followed by a relatively rapid decline. To date, various
models have been proposed to explain the afterglow emission, including a
choked-jet cocoon and a successful-jet cocoon (a.k.a. structured jet). However,
the observational data have remained inconclusive as to whether GW170817
launched a successful relativistic jet. Here we show, through Very Long
Baseline Interferometry, that the compact radio source associated with GW170817
exhibits superluminal motion between two epochs at 75 and 230 days post-merger.
This measurement breaks the degeneracy between the models and indicates that,
while the early-time radio emission was powered by a wider-angle outflow
(cocoon), the late-time emission was most likely dominated by an energetic and
narrowly-collimated jet, with an opening angle of <5 degrees, and observed from
a viewing angle of about 20 degrees. The imaging of a collimated relativistic
outflow emerging from GW170817 adds substantial weight to the growing evidence
linking binary neutron star mergers and short gamma-ray bursts.Comment: 42 pages, 4 figures (main text), 2 figures (supplementary text), 2
tables. Referee and editor comments incorporate
Modeling recursive RNA interference.
An important application of the RNA interference (RNAi) pathway is its use as a small RNA-based regulatory system commonly exploited to suppress expression of target genes to test their function in vivo. In several published experiments, RNAi has been used to inactivate components of the RNAi pathway itself, a procedure termed recursive RNAi in this report. The theoretical basis of recursive RNAi is unclear since the procedure could potentially be self-defeating, and in practice the effectiveness of recursive RNAi in published experiments is highly variable. A mathematical model for recursive RNAi was developed and used to investigate the range of conditions under which the procedure should be effective. The model predicts that the effectiveness of recursive RNAi is strongly dependent on the efficacy of RNAi at knocking down target gene expression. This efficacy is known to vary highly between different cell types, and comparison of the model predictions to published experimental data suggests that variation in RNAi efficacy may be the main cause of discrepancies between published recursive RNAi experiments in different organisms. The model suggests potential ways to optimize the effectiveness of recursive RNAi both for screening of RNAi components as well as for improved temporal control of gene expression in switch off-switch on experiments
Functional consequences of Kir2.1/Kir2.2 subunit heteromerization
Kir2 subunits form channels that underlie classical strongly inwardly rectifying potassium currents. While homomeric Kir2 channels display a number of distinct and physiologically important properties, the functional properties of heteromeric Kir2 assemblies, as well as the stoichiometries and the arrangements of Kir2 subunits in native channels, remain largely unknown. Therefore, we have implemented a concatemeric approach, whereby all four cloned Kir2 subunits were linked in tandem, in order to study the effects of Kir2.1 and Kir2.2 heteromerization on properties of the resulting channels. Kir2.2 subunits contributed stronger to single-channel conductance than Kir2.1 subunits, and channels containing two or more Kir2.2 subunits displayed conductances indistinguishable from that of a Kir2.2 homomeric channel. In contrast, single-channel kinetics was a more discriminating property. The open times were significantly shorter in Kir2.2 channels compared with Kir2.1 channels and decreased nearly proportionally to the number of Kir2.2 subunits in the heteromeric channel. Similarly, the sensitivity to block by barium also depended on the proportions of Kir2.1 to Kir2.2 subunits. Overall, the results showed that Kir2.1 and Kir2.2 subunits exert neither a dominant nor an anomalous effect on any of the properties of heteromeric channels. The data highlight opportunities and challenges of using differential properties of Kir2 channels in deciphering the subunit composition of native inwardly rectifying potassium currents
Extreme Food-Plant Specialisation in Megabombus Bumblebees as a Product of Long Tongues Combined with Short Nesting Seasons
© 2015 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ The attached file is the published version of the article
Nonlinear vortex light beams supported and stabilized by dissipation
We describe nonlinear Bessel vortex beams as localized and stationary
solutions with embedded vorticity to the nonlinear Schr\"odinger equation with
a dissipative term that accounts for the multi-photon absorption processes
taking place at high enough powers in common optical media. In these beams,
power and orbital angular momentum are permanently transferred to matter in the
inner, nonlinear rings, at the same time that they are refueled by spiral
inward currents of energy and angular momentum coming from the outer linear
rings, acting as an intrinsic reservoir. Unlike vortex solitons and dissipative
vortex solitons, the existence of these vortex beams does not critically depend
on the precise form of the dispersive nonlinearities, as Kerr self-focusing or
self-defocusing, and do not require a balancing gain. They have been shown to
play a prominent role in "tubular" filamentation experiments with powerful,
vortex-carrying Bessel beams, where they act as attractors in the beam
propagation dynamics. Nonlinear Bessel vortex beams provide indeed a new
solution to the problem of the stable propagation of ring-shaped vortex light
beams in homogeneous self-focusing Kerr media. A stability analysis
demonstrates that there exist nonlinear Bessel vortex beams with single or
multiple vorticity that are stable against azimuthal breakup and collapse, and
that the mechanism that renders these vortexes stable is dissipation. The
stability properties of nonlinear Bessel vortex beams explain the experimental
observations in the tubular filamentation experiments.Comment: Chapter of boo
Somatic mutations in exocrine pancreatic tumors: association with patient survival.
KRAS mutations are major factors involved in initiation and maintenance of pancreatic tumors. The impact of different mutations on patient survival has not been clearly defined. We screened tumors from 171 pancreatic cancer patients for mutations in KRAS and CDKN2A genes. Mutations in KRAS were detected in 134 tumors, with 131 in codon 12 and only 3 in codon 61. The GGT>GAT (G12D) was the most frequent mutation and was present in 60% (80/134). Deletions and mutations in CDKN2A were detected in 43 tumors. Analysis showed that KRAS mutations were associated with reduced patient survival in both malignant exocrine and ductal adenocarcinomas (PDAC). Patients with PDACs that had KRAS mutations showed a median survival of 17 months compared to 30 months for those without mutations (log-rank P = 0.07) with a multivariate hazard ratio (HR) of 2.19 (95%CI 1.09-4.42). The patients with G12D mutation showed a median survival of 16 months (log-rank-test P = 0.03) and an associated multivariate HR 2.42 (95%CI 1.14-2.67). Although, the association of survival in PDAC patients with CDKN2A aberrations in tumors was not statistically significant, the sub-group of patients with concomitant KRAS mutations and CDKN2A alterations in tumors were associated with a median survival of 13.5 months compared to 22 months without mutation (log-rank-test P = 0.02) and a corresponding HR of 3.07 (95%CI 1.33-7.10). Our results are indicative of an association between mutational status and survival in PDAC patients, which if confirmed in subsequent studies can have potential clinical application
DMD: A Large-Scale Multi-Modal Driver Monitoring Dataset for Attention and Alertness Analysis
Vision is the richest and most cost-effective technology for Driver
Monitoring Systems (DMS), especially after the recent success of Deep Learning
(DL) methods. The lack of sufficiently large and comprehensive datasets is
currently a bottleneck for the progress of DMS development, crucial for the
transition of automated driving from SAE Level-2 to SAE Level-3. In this paper,
we introduce the Driver Monitoring Dataset (DMD), an extensive dataset which
includes real and simulated driving scenarios: distraction, gaze allocation,
drowsiness, hands-wheel interaction and context data, in 41 hours of RGB, depth
and IR videos from 3 cameras capturing face, body and hands of 37 drivers. A
comparison with existing similar datasets is included, which shows the DMD is
more extensive, diverse, and multi-purpose. The usage of the DMD is illustrated
by extracting a subset of it, the dBehaviourMD dataset, containing 13
distraction activities, prepared to be used in DL training processes.
Furthermore, we propose a robust and real-time driver behaviour recognition
system targeting a real-world application that can run on cost-efficient
CPU-only platforms, based on the dBehaviourMD. Its performance is evaluated
with different types of fusion strategies, which all reach enhanced accuracy
still providing real-time response.Comment: Accepted to ECCV 2020 workshop - Assistive Computer Vision and
Robotic
Analysis of neural crest-derived clones reveals novel aspects of facial development
Cranial neural crest cells populate the future facial region and produce ectomesenchyme-derived tissues, such as cartilage, bone, dermis, smooth muscle, adipocytes, and many others. However, the contribution of individual neural crest cells to certain facial locations and the general spatial clonal organization of the ectomesenchyme have not been determined. We investigated how neural crest cells give rise to clonally organized ectomesenchyme and how this early ectomesenchyme behaves during the developmental processes that shape the face. Using a combination of mouse and zebrafish models, we analyzed individual migration, cell crowd movement, oriented cell division, clonal spatial overlapping, and multilineage differentiation. The early face appears to be built from multiple spatially defined overlapping ectomesenchymal clones. During early face development, these clones remain oligopotent and generate various tissues in a given location. By combining clonal analysis, computer simulations, mouse mutants, and live imaging, we show that facial shaping results from an array of local cellular activities in the ectomesenchyme. These activities mostly involve oriented divisions and crowd movements of cells during morphogenetic events. Cellular behavior that can be recognized as individual cell migration is very limited and short-ranged and likely results from cellular mixing due to the proliferation activity of the tissue. These cellular mechanisms resemble the strategy behind limb bud morphogenesis, suggesting the possibility of common principles and deep homology between facial and limb outgrowth
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