Isolation and characterization of metal resistant-tolerant rhizosphere bacteria from the serpentine soils in Turkey

Despite the number of studies describing metal hyper-accumulating plants and their associated bacteria in various regions and countries, there is no information on rhizosphere microbial potential of the Turkish serpentine soils. This study aimed to explore the rhizosphere microbial diversity of Ni-resistant, hyper-accumulating plants grown on Ni-rich soils and their metal tolerance-resistance characteristics. One hundred ninety-one locations were visited to collect soil and plant samples from different serpentine regions of Western Turkey. Following bioavailable and total Ni analysis of collected samples, the seeds of the selected plants with higher Ni content were taken to the growth/germination test in a range of serpentine soils in a growth chamber condition. In order to investigate the rhizosphere microbial diversity, Isatis pinnatiloba and Alyssum dasycarpum which were able to germinate and grow well in the preliminary tests, were introduced to 6-month greenhouse experiment in the range of three serpentine soils with higher bioavailable Ni content. I. pinnatiloba had a better stimulatory effect on the rhizosphere microbial diversity. A total of 22 bacterial isolates were identified from different soil conditions in the end of experiment. Following microbial identification and confirmation tests, 11 isolates were found to be resistant and tolerant to the increasing concentrations of Ni, Pb, Cd and Zn in the range of 50-2,000 mg L (-aEuro parts per thousand 1), which was considerably higher than those indicated by earlier studies. The strains isolated and identified from the Turkish serpentine soils were the members of genera Arthrobacter, Bacillus, Microbacterium and Staphylococcus

Total phenolics, mineral elements, antioxidant and antibacterial activities of some edible wild plants in Turkey

Total phenolic content, macro and micro elements (N, P, K, Ca, Mg, Na, Fe, Mn, Zn and Cu), antioxidant and antibacterial activities of leaves of some edible plants (Sinapis arvensis L., Polygonum aviculare L. and Tragopogon aureus Boiss.) grown wild in Eastern Anatolia region of Turkey were studied. The total phenolic content was found between 12.56 mu gGAE/mg (T. aureus) and 22.25 mu gGAE/mg (S. arvensis). All three species showed high antioxidant activity and among them T aureus possessed the highest one (88.64 %). There was a low correlation (R = 0.447) between total phenolic content and antioxidant activity in the plant samples. N, P and K values of plants were between 1.98 % (T aureus) and 3.44 % (S. arvensis); 164 mg/100 g (T aureus) to 255 mg/100 g (S. arvensis) and 2005 mg/100 g (P aviculare) to 2710 mg/100 g (T aureus), respectively. The methanol extracts of the leaves of species showed antibacterial activities against a number of microorganisms searched. However, none of the water extracts of plants showed antibacterial activity on the microorganisms studied

Levels of obestatin in euthyroid patients receiving levothyroxine replacement therapy

To investigate the relationship between obestatin and TSH levels in patients who received thyroid hormone replacement and were in the euthyroid state. Included 30 patients who were followed up in the endocrinology outpatient clinic in 2020, who received L-thyroxine replacement, and 30 healthy individuals without any chronic disease who did not receive thyroid hormone replacement. Blood samples were taken from both groups for obestatin, thyroid-stimulating hormone (TSH) and free T4 hormone (fT4) levels. There is significant difference in TSH levels (p = 0.007) was found between the two groups. The obestatin levels of the L-thyroxine replacement group were lower than those of the control group (p = 0.001). No correlation was observed between the TSH and obestatin levels in the control and L-thyroxine replacement groups. In this study, obestatin levels were significantly lower in patients who received L-thyroxine replacement therapy and were in euthyroid status than those in the control group. No correlation was found between obestatin and TSH levels in the control and patient groups. As a result of this study, the thyroid gland may play a more important role in the synthesis or regulation of obestatin synthesis beyond our current knowledge. [Med-Science 2022; 11(1.000): 131-4

Total phenolics, mineral contents, antioxidant and antibacterial activities of Glycyrrhiza glabra L. roots grown wild in Turkey

The total phenolic content, macro and micro elements (N, P, K, Ca, Mg, Na, Fe, Mn, Zn and Cu), antioxidant and antibacterial activities of roots of wild grown Glycyrrhiza glabra are reported. The amount of total phenolics was 12.88 mu gGAF-/mg DW in Glycyrrhiza glabra roots. The average N, P, K, Ca, Mg, Fe, Mn, Zn, Na and Cu composition in licorice roots was 2.80%, 175 mg/100 g, 1400 mg/100 g, 147 mg/100 g, 120 mg/100 g, 20 mg/100 g, 6 mg/100 g, 4.4 mg/100 g, 2.1 mg/100 g and 0.1 mg/100 g, respectively. The highest antioxidant activity of root extract was 88.7% at a concentration of 800 mu g/mL. The methanol extracts of the roots showed antibacterial activities against Agrobacterium tumefaciens, Bacillus cereus, Bacillus subtilus and Pseudomonas syringae pv. tomato, but none of the water extracts of Glycyrrhiza glabra roots showed any antibacterial activity against the these microorganisms

The hepatoprotective potential of resveratrol in an experimental model of ruptured abdominal aortic aneurysm via oxidative stress and apoptosis

The mortality rate in ruptured abdominal aortic aneurysms can today be reduced through cardiovascular surgery. However, ischemia and reperfusion-induced tissue damage develop due to aortic cross-clamping applied during surgery. The present study aimed to reduce oxidative stress-induced hepatic damage resulting from ischemia and reperfusion due to aortic cross-clamping during surgery by means of resveratrol administration. Forty male Sprague-Dawley rats were randomly assigned into four groups: control (healthy), glycerol+ischemia/reperfusion (I/R) (sham), I/R, and I/R + Resveratrol. In all groups scheduled for I/R, 60 min of shock was followed by 60 min of ischemia. In the I/R + Resveratrol group, 10 mg/kg of resveratrol was administered 15 min before ischemia and immediately before reperfusion via the intraperitoneal route. In addition, 120 min of reperfusion was applied under anesthesia after ischemia in all groups. Intralobar and interlobar necrosis, vascular congestion, and edematous fields resulting from aortic occlusion were present. Liver tissue malondialdehyde (MDA) levels and cleaved caspase-3 positivity increased, while glutathione (GSH) levels decreased. However, resveratrol administration reduced intralobular and interlobar necrosis, vascular congestion and edematous fields, cleaved caspase-3 positivity, and MDA levels, and increased GSH levels. Our findings suggest that resveratrol is effective against aortic occlusion-induced liver injury by reducing oxidative stress and apoptosis

Early administration of milrinone ameliorates lung and kidney injury during sepsis in juvenile rats

Background A sepsis model was created, induced by cecal ligation and puncture (CLP), in juvenile rat groups. Milrinone (MIL), which is known to have a modulatory effect on pro-inflammatory cytokines, was administered to the designated rat groups in the early period before severe sepsis developed. The study was aimed at investigating the possible protective effects of milrinone on the lung and kidney tissues of rats in the late phase of sepsis. Methods The rat pups were divided into seven groups with six animals in each group: (1) healthy rats who received no drug; (2) CLP-S12 (sacrificed at hour 12); (3) CLP-S24 (sacrificed at hour 24); (4) CLP-MIL1-S12 (administered with 0.5 mg/kg milrinone at hour 1 and sacrificed at hour 12); (5) CLP-MIL1-S24 (administered with 0.5 mg/kg milrinone at hour 1 and sacrificed at hour 24): (6) CLP-MIL12-S24 (administered with 0.5 mg/kg milrinone at hour 12 and sacrificed at hour 24), (7) and CLP-MIL1,12-S24 (administered with 0.5 mg/kg milrinone at hours 1 and 12 and sacrificed at hour 24). Results Significant differences were found between the early and late administration of milrinone in terms of both molecular and histopathological results. The results showed that the tissues were significantly preserved in the groups in which milrinone had been started in the early period compared to the sepsis control groups and the groups in which milrinone had been started in the late period. Conclusions In addition to the positive inotropic effects of milrinone, its immunomodulatory properties that result in decreased cytokine storm can be beneficial during early period of sepsis