Comparison of methods for determining the fatty acid composition of photosynthetic tissues

The fatty acid (FA) composition of photosynthetic tissue differs from that in other plant or animal tissues. In leaves, the lipid fraction constitutes less than 10% of the dry weight and is mostly located in the chloroplasts. An extraction solvent should dissolve polar lipids readily, but should also overcome interactions between the lipids and the tissue matrix. A mixture of chloroform/methanol (C/M) is commonly used. However, less toxic alternative methods such as hexane/isopropanol (H/I) and ethanol (E) have been suggested. In this preliminary study we compared the effectiveness of these three methods which are used as standard extraction protocols for FA analysis of plant material at three different European Universities. C/M extraction gave the highest total FA content and H/I the lowest, suggesting that C/M is indeed the best general-purpose lipid extraction solvent. Significant differences were also observed for FA composition including the ratio of saturated to unsaturated FA indicating selectivity of the various solvents in extracting different individual FA. Further and more detailed investigations are required to confirm this hypothesi

The effect of N-fertilisation rate or inclusion of red clover to timothy leys on fatty acid composition in milk of dairy cows fed a commercial silage:concentrate ratio

The aim of this experiment was to, under typical Swedish production conditions, evaluate the effects of grass silages subjected to different N-fertilisation regimes fed to dairy cows on the fatty acid (FA) composition of their milk, and to compare the grass silages in this respect to red clover-dominated silage. Grass silages made from first year Phleum pratense L. leys subjected to three N-fertilisation regimes (30, 90 and 120 kg N/ha, designated G-30, G-90 and G-120, respectively) and a mixed red clover grass silage (Trifolium pratense L. and P. pratense L; 60/40 on dry matter (DM) basis, designated RC G) were produced. The experiment was conducted as a change-over design, including 24 primiparous and multiparous dairy cows of the Swedish Red breed, each of which was allocated to three of the four diets. The cows were offered 11 kg DM of silage and 7 kg concentrates. The silages had similar DM and energy concentrations. The CP concentration increased with increase in N-fertilisation level. There was a linear increase in DM intake of the different silages with increased N fertilisation. There were also differences in concentrations of both individual and total FAs amongst silages. The daily milk production (kg/day) did not significantly differ between treatments, but G-30 silage resulted in higher concentrations of 18:2n-6 in the milk compared with the other two grass silages. The highest concentrations of 18:3n-3 and cis-9, trans-11 18:2 were found in milk from cows offered the RC G silage. The G-30 diet resulted in higher concentration of 18:2n-6 and the same concentration of 18:3n-3 in the milk as the other grass silages, despite lower intake levels of these FAs. The apparent recoveries of 18:3n-3 from feed to milk were 5.74%, 4.27%, 4.10% and 5.31% for G-30, G-90, G-120 and RC G, respectively. A higher recovery when red clover is included in the diet confirms previous reports. The higher apparent recovery of 18:3n-3 on the G-30 treatment may be related to the lower silage DM intake, which led to a higher relative proportion of ingested FAs originating from concentrates compared with the G-90 and G-120 diets. With the rates and types of concentrates used in this study, the achieved differences in FA composition among the silages were not enough to influence the concentrations of unsaturated FAs in milk

On instantons as Kaluza-Klein modes of M5-branes

Instantons and W-bosons in 5d maximally supersymmetric Yang-Mills theory arise from a circle compactification of the 6d (2,0) theory as Kaluza-Klein modes and winding self-dual strings, respectively. We study an index which counts BPS instantons with electric charges in Coulomb and symmetric phases. We first prove the existence of unique threshold bound state of (noncommutative) U(1) instantons for any instanton number, and also show that charged instantons in the Coulomb phase correctly give the degeneracy of SU(2) self-dual strings. By studying SU(N) self-dual strings in the Coulomb phase, we find novel momentum-carrying degrees on the worldsheet. The total number of these degrees equals the anomaly coefficient of SU(N) (2,0) theory. We finally show that our index can be used to study the symmetric phase of this theory, and provide an interpretation as the superconformal index of the sigma model on instanton moduli space.Comment: 54 pages, 2 figures. v2: references added, figure improved, added comments on self-dual string anomaly, added new materials on the symmetric phase index, other minor correction

Sequential and Spontaneous Star Formation Around the Mid-Infrared Halo HII Region KR 140

We use 2MASS and MSX infrared observations, along with new molecular line (CO) observations, to examine the distribution of young stellar objects (YSOs) in the molecular cloud surrounding the halo HII region KR 140 in order to determine if the ongoing star-formation activity in this region is dominated by sequential star formation within the photodissociation region (PDR) surrounding the HII region. We find that KR 140 has an extensive population of YSOs that have spontaneously formed due to processes not related to the expansion of the HII region. Much of the YSO population in the molecular cloud is concentrated along a dense filamentary molecular structure, traced by C18O, that has not been erased by the formation of the exciting O star. Some of the previously observed submillimetre clumps surrounding the HII region are shown to be sites of recent intermediate and low-mass star formation while other massive starless clumps clearly associated with the PDR may be the next sites of sequential star formation.Comment: Accepted for publication in MNRAS, 8 pages, 10 figure

Cost-effectiveness of alternative methods of surgical repair of inguinal hernia

Objectives: To assess the relative cost-effectiveness of laparoscopic methods of inguinal hernia repair compared with open flat mesh and open non-mesh repair. Methods: Data on the effectiveness of these alternatives came from three systematic reviews comparing: (i) laparoscopic methods with open flat mesh or non-mesh methods; (ii) open flat mesh with open non-mesh repair; and (iii) methods that used synthetic mesh to repair the hernia defect with those that did not. Data on costs were obtained from the authors of economic evaluations previously conducted alongside trials included in the reviews. A Markov model was used to model cost-effectiveness for a five-year period after the initial operation. The outcomes of the model were presented using a balance sheet approach and as cost per hernia recurrence avoided and cost per extra day at usual activities. Results: Open flat mesh was the most cost-effective method of preventing recurrences. Laparoscopic repair provided a shorter period of convalescence and less long-term pain compared with open flat mesh but was more costly. The mean incremental cost per additional day back at usual activities compared with open flat mesh was €38 and €80 for totally extraperitoneal and transabdominal preperitoneal repair, respectively. Conclusions: Laparoscopic repair is not cost-effective compared with open flat mesh repair in terms of cost per recurrence avoided. Decisions about the use of laparoscopic repair depend on whether the benefits (reduced pain and earlier return to usual activities) outweigh the extra costs and intraoperative risks. On the evidence presented here, these extra costs are unlikely to be offset by the short-term benefits of laparoscopic repair.Luke Vale, Adrian Grant, Kirsty McCormack, Neil W. Scott and the EU Hernia Trialists Collaboratio

Effect of sampling rate on acceleration and counts of hip- and wrist-worn ActiGraph accelerometers in children

Sampling rate (Hz) of ActiGraph accelerometers may affect processing of acceleration to activity counts when using a hip-worn monitor, but research is needed to quantify if sampling rate affects actual acceleration (mg's), when using wrist-worn accelerometers and during non-locomotive activities. Objective: To assess the effect of ActiGraph sampling rate on total counts/15-sec and mean acceleration and to compare differences due to sampling rate between accelerometer wear locations and across different types of activities. Approach: Children (n=29) wore a hip- and wrist-worn accelerometer (sampled at 100 Hz, downsampled in MATLAB to 30 Hz) during rest/transition periods, active video games, and a treadmill test to volitional exhaustion. Mean acceleration and counts/15-sec were computed for each axis and as vector magnitude. Main Results: There were mostly no significant differences in mean acceleration. However, 100 Hz data resulted in significantly more total counts/15-sec (mean bias 4-43 counts/15-sec across axes) for both the hip- and wrist-worn monitor when compared to 30 Hz data. Absolute differences increased with activity intensity (hip: r=0.46-0.63; wrist: r=0.26-0.55) and were greater for hip- versus wrist-worn monitors. Percent agreement between 100 and 30 Hz data was high (97.4-99.7%) when cut-points or machine learning algorithms were used to classify activity intensity. Significance: Our findings support that sampling rate affects the generation of counts but adds that differences increase with intensity and when using hip-worn monitors. We recommend researchers be consistent and vigilantly report the sampling rate used, but note that classifying data into activity intensities resulted in agreement despite differences in sampling rate

A Sample of Intermediate-Mass Star-Forming Regions: Making Stars at Mass Column Densities <1 g/cm^2

In an effort to understand the factors that govern the transition from low- to high-mass star formation, we identify for the first time a sample of intermediate-mass star-forming regions (IM SFRs) where stars up to - but not exceeding - 8 solar masses are being produced. We use IRAS colors and Spitzer Space Telescope mid-IR images, in conjunction with millimeter continuum and CO maps, to compile a sample of 50 IM SFRs in the inner Galaxy. These are likely to be precursors to Herbig AeBe stars and their associated clusters of low-mass stars. IM SFRs constitute embedded clusters at an early evolutionary stage akin to compact HII regions, but they lack the massive ionizing central star(s). The photodissociation regions that demarcate IM SFRs have typical diameters of ~1 pc and luminosities of ~10^4 solar luminosities, making them an order of magnitude less luminous than (ultra)compact HII regions. IM SFRs coincide with molecular clumps of mass ~10^3 solar masses which, in turn, lie within larger molecular clouds spanning the lower end of the giant molecular cloud mass range, 10^4-10^5 solar masses. The IR luminosity and associated molecular mass of IM SFRs are correlated, consistent with the known luminosity-mass relationship of compact HII regions. Peak mass column densities within IM SFRs are ~0.1-0.5 g/cm^2, a factor of several lower than ultra-compact HII regions, supporting the proposition that there is a threshold for massive star formation at ~1 g/cm^2.Comment: 61 pages, 6 tables, 20 figures. Accepted for publication in the Astronomical Journa