Anderbrant, Olle

Arvidsson, A. K.

Munyanez, Joseph E

Nissinen, A.

Rämert, B.

Sengoda, V. G.

Stegmark, R.

Yuvaraj, Jothi Kumar

English

Lund University Publications

LUND UNIVERSITYPO Box 117221 00 Lund+46 46-222 00 00First report of "Candidatus Liberibacter solanacearum" associated with psyllid-affected carrots in SwedenMunyanez, Joseph E; Sengoda, V. G.; Stegmark, R.; Arvidsson, A. K.; Anderbrant, Olle;Yuvaraj, Jothi Kumar; Rämert, B.; Nissinen, A.Published in:Plant DiseaseDOI:10.1094/PDIS-10-11-08712012Link to publicationCitation for published version (APA):Munyanez, J. E., Sengoda, V. G., Stegmark, R., Arvidsson, A. K., Anderbrant, O., Yuvaraj, J. K., Rämert, B., &Nissinen, A. (2012). First report of "Candidatus Liberibacter solanacearum" associated with psyllid-affectedcarrots in Sweden. Plant Disease, 96(3), 453-453. https://doi.org/10.1094/PDIS-10-11-0871Total number of authors:8General rightsUnless other specific re-use rights are stated the following general rights apply:Copyright and moral rights for the publications made accessible in the public portal are retained by the authorsand/or other copyright owners and it is a condition of accessing publications that users recognise and abide by thelegal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private studyor research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portalRead more about Creative commons licenses: https://creativecommons.org/licenses/Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will removeaccess to the work immediately and investigate your claim.Welcome Sign in | Register Journals Home APSnet IS-MPMInet My Profile Help Subscribe Search   Advanced SearchISSN: 0191-2917SEARCH PhytopathologyPlant DiseaseMPMI  Advanced SearchEnter KeywordsInside the JournalBACK ISSUES(Issues before 1997) First LookView Most Downloaded ArticlesAbout Plant DiseaseEditorial BoardSubmit a ManuscriptAuthor InstructionsPolicies/ProceduresOnline e-Xtras = "Open" Access Editor-in-Chief: R. Michael DavisPublished by The American Phytopathological SocietyPrevious Article  |  Next Article March 2012, Volume 96, Number 3Page 453http://dx.doi.org/10.1094/PDIS-10-11-0871 Disease Notes First Report of “Candidatus Liberibacter solanacearum” Associated with Psyllid-Affected Carrots in SwedenJ. E. Munyaneza and V. G. Sengoda, USDA-ARS, Yakima Agricultural Research Laboratory, Wapato, WA 98951; R. Stegmark and A. K. Arvidsson, Findus Sverige AB, SE-267 81 Bjuv, Sweden; O. Anderbrantand J. K. Yuvaraj, Lund University, Department of Biology, SE-223 62 Lund, Sweden; B. Rämert, Swedish University of Agricultural Sciences, Plant Protection Biology, SE-230 53 Alnarp, Sweden; and A. Nissinen, MTT Agrifood Research Finland, Plant Production Research, FI-31600 Jokioinen, FinlandCarrot (Daucus carota) plants with symptoms resembling those associated with the carrot psyllid Trioza apicalis and the bacterium “CandidatusLiberibacter solanacearum” (1–4) were observed in 70% of commercial fields in southern Sweden in August 2011, with approximately 1 to 45% symptomatic plants per field. T. apicalis, a pest of carrot in northern and central Europe, including Sweden, can cause as much as 100% crop loss and is associated with “Ca. L. solanacearum” (1–4). Symptoms on affected plants include leaf curling, yellow and purple discoloration of leaves, stunted growth of shoots and roots, and proliferation of secondary roots (3). Carrot plant and psyllid samples were collected from fields in the province of Halland. Total DNA was extracted from petiole and root tissues of 33 symptomatic and 16 asymptomatic plants (cvs. Nevis and Florida), with the cetyltrimethylammonium bromide (CTAB) buffer extraction method (2,3). DNA was also extracted from 155 psyllids (3). DNA samples were tested by PCR using primer pairs OA2/OI2c (5′-GCGCTTATTTTTAATAGGAGCGGCA-3′/5′-GCCTCGCGACTTCGCAACCCAT-3′) and CL514F/R (5′-CTCTAAGATTTCGGTTGGTT-3′/5′-TATATCTATCGTTGCACCAG-3′), to amplify a portion of 16S rDNA and rplJ/rplL ribosomal protein genes, respectively, of “Ca. L. solanacearum” (2,3). A 1,168-bp 16S rDNA fragment was detected in the DNA from all 33 symptomatic and two asymptomatic plants, and a 668-bp rplJ/rplL fragment was amplified from the DNA of all 33 symptomatic and four asymptomatic plants, indicating the presence of liberibacter. DNA from 23 and 49 psyllid samples yielded similar amplicons with OA2/OI2c and CL514F/R primer pairs, respectively. Amplicons from the DNA of four carrot roots and three T. apicalis with each primer pair were cloned (pCR2.1-TOPO; Invitrogen, Carlsbad, CA) and three clones of each of the 14 amplicons were sequenced (MCLAB, San Francisco, CA). BLAST analysis of the 16S rDNA consensus sequences from carrot (GenBank Accession No. JN863095) and T. apicalis (GenBank Accession No. NJ863096) showed 100% identity to those Home > Plant Disease  > Table of Contents  > Abstract Quick LinksAdd to favoritesE-mail to a colleague Alert me when new articles cite this articleDownload to citation managerRelated articles found in APS JournalsPage 1 of 2Plant Disease - 96(3):453 - Abstract2/9/2012http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-10-11-0871Journals Home APSnet IS-MPMInet Contact Us Privacy Copyright The American Phytopathological Society of “Ca. L. solanacearum” previously amplified from carrot (GU373048 and GU373049) and T. apicalis (GU477254 and GU477255) from Finland (2,3). The rplJ/rplL consensus sequences from carrot (GenBank Accession No. JN863093) and T. apicalis (GenBank Accession No. JN863094) were 99% identical to the sequences of rplJ/rplL “Ca. L. solanacearum” ribosomal protein gene from carrots in Finland (GU373050 and GU373051). To our knowledge, this is the first report of “Ca. L. solanacearum” associated with carrot and T. apicalis in Sweden. The disease associated with this bacterium caused millions of dollars in losses to potato and several other solanaceous crops in North and Central America and New Zealand (1). This plant pathogen is also associated with significant economic damage to carrot crops observed in Finland (2,3).References: (1) J. E. Munyaneza. Southwest. Entomol. 35:471, 2010. (2) J. E. Munyaneza et al. Plant Dis. 94:639, 2010. (3) J. E. Munyaneza et al. J. Econ. Entomol. 103:1060, 2010. (4) A. Nissinen et al. Entomol. Exp. Appl. 125:277, 2007.Page 2 of 2Plant Disease - 96(3):453 - Abstract2/9/2012http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-10-11-0871Journals Home APSnet IS-MPMInet Contact Us Privacy Copyright The American Phytopathological Society Welcome Sign in | Register Journals Home APSnet IS-MPMInet My Profile Help Subscribe Search   Advanced SearchISSN: 0191-2917SEARCH PhytopathologyPlant DiseaseMPMI  Advanced SearchEnter KeywordsInside the JournalBACK ISSUES(Issues before 1997) First LookView Most Downloaded ArticlesAbout Plant DiseaseEditorial BoardSubmit a ManuscriptAuthor InstructionsPolicies/ProceduresOnline e-Xtras = "Open" Access Editor-in-Chief: R. Michael DavisPublished by The American Phytopathological SocietyPrevious Article  |  Next Article March 2012, Volume 96, Number 3Page 453http://dx.doi.org/10.1094/PDIS-10-11-0871 Supplemental MaterialCarrots exhibiting symptoms associated with the psyllid Trioza apicalis and the bacterium “Candidatus Liberibacter solanacearum”: leaf curling and discoloration (left), leaf curling only (center), and healthy carrots (right).Home > Plant Disease  > Table of Contents  > Supplemental Material Quick LinksAdd to favoritesE-mail to a colleague Alert me when new articles cite this articleDownload to citation managerRelated articles found in APS JournalsPage 1 of 1Plant Disease - 96(3):453 - Supplemental Material2/9/2012http://apsjournals.apsnet.org/doi/suppl/10.1094/PDIS-10-11-0871

First report of "Candidatus Liberibacter solanacearum" associated with psyllid-affected carrots in Sweden

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First report of "Candidatus Liberibacter solanacearum" associated with psyllid-affected carrots in Sweden

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