Overview Of Data Over Digital Subscrieber Line In Czech Republic.

Import 22/07/2015Tato bakalářská práce je zaměřena na rozbor technologie ADSL a to, jak je využívána a nabízena vybranými poskytovateli v České republice k užívání veřejným sektorem. V teoretické části pojednává o technologii ADSL a jejích vývojových verzích. Dále se zabývá řešením přípojky a vlivů rušení na vedení těchto přípojek. Hlavní částí práce je část praktická, ta se zabývá průzkumem poskytovatelů v České republice. Konkrétně využitím digitálních účastnických smyček poskytovateli, jejich nabídkou ADSL služeb pevného připojení k internetu a také doplňkových služeb a poplatků s pevným připojením spojených. Poslední částí práce je analýza možností připojení těchto služeb na konkrétní přípojku. Obsah této práce má napomoci běžným uživatelům k pochopení dané problematiky spojené se zřízením a výběrem ADSL služeb. Prostředky k řešení praktické části práce jsou především komunikace s poskytovateli ADSL služeb a získávání informací.This bachelor thesis is focused on the analysis of the technology ADSL as it is used in the public sector and offered by chosen providers in Czech Republic. In the theoretical part, it refers to the technology ADSL and its developmental versions. Furthermore, it examines the connection solution and the influence of interruption on conducting these connections. The main part of the thesis is the practical part which undertakes the research of providers in Czech Republic. Namely, it explores use of digital subscriber loops by providers, theirs ADSL service offers of the stable internet connection and its complementary services and charges. The final part of the thesis is the connection analysis of these complementary services on the specific connection. The content of this thesis should provide help to common users to understand given issues related to the selection and setting up ADSL services. Tools used for the practical part are particularly communication with ADSL service providers and acquiring appropriate information.440 - Katedra telekomunikační technikyvýborn

Synthesis, characterization, and catalytic oxidation of styrene, cyclohexene, allylbenzene, and <i>cis</i>-cyclooctene by recyclable polymer-grafted Schiff base complexes of vanadium(IV)

<p>Schiff base-functionalized chloromethylated polystyrenes, PS-[Ae-Eol] (<b>I</b>), PS-[Hy-Eda] (<b>II</b>) and PS-[HyP-Eda] (<b>III</b>), were synthesized by reacting 2-(2-aminoethoxy)ethanol (Ae-Eol), N-(2-hydroxyethyl)ethylenediamine (Hy-Eda), and N-(2-hydroxpropyl)ethylenediamine (HyP-Eda) with oxidized chloromethylated polystyrene. Oxidized chloromethylated polystyrene (PS-CHO) was prepared by oxidation of chloromethylated polystyrene (PS) with sodium bicarbonate in DMSO. By reacting DMSO solution of [VO(acac)₂] with polymer-anchored Schiff base ligands <b>I</b>, <b>II</b>, and <b>III</b>, vanadium(IV) complexes PS-[V^IVO(Ae-Eol)] (<b>1</b>), PS-[V^IVO(Hy-Eda)] (<b>2</b>), and PS-[V^IVO(HyP-Eda)] (<b>3</b>) were prepared. Structure and bonding of <b>I</b>, <b>II</b>, and <b>III</b> as well as corresponding vanadium complexes <b>1</b>, <b>2</b>, and <b>3</b> were confirmed by FT-IR, UV–vis spectroscopy, SEM, EDX, AAS, TGA, EPR, <i>etc</i>. Polymer-anchored vanadium(IV) complexes <b>1</b>, <b>2</b>, and <b>3</b> show, efficient catalysis toward oxidation of styrene, cyclohexene, allylbenzene, and <i>cis</i>-cyclooctene in the presence of hydrogen peroxide. Optimized reaction conditions for the oxidation of these alkenes was achieved by changing various reaction parameters (like amount of catalyst, amount of oxidizing agent, volume of solvent, <i>etc.</i>). Polymer-grafted <b>1</b>, <b>2</b>, and <b>3</b> can be reused multiple times without depletion of their activity.</p

Biolayer inferometry (BLItz) of zebrafish proteins.

<p>Scatchard plot showing the binding of zRET^ecd and zGDNF to pre-incubated zGFRα1 using BLItz. The x-axis shows the concentration of zGDNF and zRET^ecd. The dashed line indicates the nonlinear curve fit plotted by GraphPad Prism 6 for single-site binding.</p

zGDNF activates hRET phosphorylation as shown by anti-phospho-RET western blotting.

<p>The anti-phosphotyrosine (pY) Western blotting reflects the level of phosphorylated tyrosine residues in RET (upper panels); anti-RET western blotting (lower panels) demonstrates equal loading in different samples. hGDNF^IC, hGDNF^BV and hGDNF^Ec produced hGDNF were used as controls at a range of concentrations stated in nM and media as a negative control.</p

Zebrafish GDNF alone and with zGFRα1 potently activated the human RET MAPK signaling.

<p>(A) luciferase activity readouts comparing different concentrations of zGDNF, using hGDNF^BV, hGDNF^Ec and hGDNF^IC as positive controls. Yellow bar: hGDNF^Ec, green bar: hGDNF^BV; red bar: hGDNF^IC; black bar: zGDNF. (B) luciferase activity readouts comparing zGDNF and zGFRα1 activation at different concentrations on the MG87 cell-line expressing RET only. Media, zGDNF and His-tagged zGFRα1 individually were used as negative controls. N = 3–4 repeats per experiment; statistically different from control <i>*p</i>< 0.01; ** <i>p</i><0.0001. Error bars indicate SEM.</p

zGDNF supports the survival of cultured mammalian dopaminergic neurons.

<p>(A) the counted number of embryonic dopaminergic neurons in the presence of zGDNF and hGDNF^IC versus negative control (media), (B) immunofluorescent staining of midbrain culture on 5DIV with a marker for DA neurons: TH (red) and nuclear dye DAPI (blue) comparing 0.27 nM of hGDNF^IC, zGDNF and no GFL (control). Black bars: zGDNF; red bars: hGDNF^IC; white bar: negative control. N = 4 indiviaul experiments per condition; statistically different from control ***p< 0.0007, **p< 0.003, *p<0.03.</p

Amino acid sequence alignment of zebrafish, human, rat, frog and fruit fly GFRα1.

<p>The red letters indicate sequence identity and blue letter sequence similarity. Dashed box indicates signal sequence. The dotted line indicates the start and end of domains 2 and 3. Solid box indicates the amino acid E323/D324 (rat), E326/E327 (zebrafish), D321/D322 (frog) predicted to be involved in hRET complex formation.</p

Sequence identity comparisons of the amino acid sequences of human, zebrafish, frog and fruit fly RET.

<p>Sequence identity comparisons of the amino acid sequences of human, zebrafish, frog and fruit fly RET.</p