Masculinidades y feminidades en preadolescentes residentes en contextos rurales de Castilla-La Mancha

Este artículo presenta los principales resultados obtenidos del análisis cuantitativo exploratorio perteneciente a la investigación Las fronteras de la identidad de género: masculinidades y feminidades en contextos de interacción escolar. Se analizan 236 cuestionarios aplicados a preadolescentes de entornos educativos, de entre 11-16 años, residentes en poblaciones rurales de la provincia de Albacete. El cuestionario indaga en algunos de los diferentes aspectos que contribuyen al proceso de adquisición y expresión de la identidad de género de estos jóvenes, constituyendo agentes socializadores de primer orden. Los resultados del estudio indican que, a pesar de prevalecer discursos igualitarios en la sociedad, sigue predominando en las personas adolescentes de estos entornos una visión y una expresión de género estereotipada de lo que supone ser hombre o mujer. Por otro lado, determinados contenidos audiovisuales que gran parte de los y las adolescentes consumen pueden influir tanto en las relaciones entre distintos géneros como en la carencia de un espíritu crítico, ya que dichos contenidos no son apropiados para estas edades y muestran unos modelos de hombres y mujeres estereotipados

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field