The contribution of OCTN1/2 variants within the IBD5 locus to disease susceptibility and severity in Crohn's disease

Background and Aims: Recent data suggest that polymorphisms in the organic cation transporter (OCTN) genes OCTN1 (SLC22A4) and OCTN2 (SLC22A5) represent disease-causing mutations within the IBD5 locus (chromosome 5q31). We investigated associations with disease susceptibility, phenotype, and evidence for epistasis with CARD15 in 679 patients with Crohn’s disease (CD) or ulcerative colitis (UC). Methods: A total of 374 patients with CD, 305 patients with UC, and 294 healthy controls (HCs) were studied. Genotyping for single nucleotide polymorphisms IGR2096, IGR2198, and IGR2230, OCTN1 variant (SLC22A4 1672C→T), and OCTN2 variant (SLC22A5 −207G→C) was performed using the TaqMan system. Results: The IBD5 OCTN1 and OCTN2 polymorphisms were in strong linkage disequilibrium (D′, >0.959). IGR2198 variant allele frequency (49.1% vs 40.8%; P = .0046) and homozygosity (21% vs 14.8%; P = .044) were associated with CD versus HCs. Variant allelic frequency of OCTN1 (53.6% vs 43%; P = .0008) and OCTN2 (56.1% vs 48.4%; P = .0092) polymorphisms and homozygosity for the OCTN1/2-TC haplotype (28.4% vs 16%; P = .0042) were associated with CD versus HCs. IGR2198 homozygosity and TC homozygosity were associated with stricturing/penetrating disease at follow-up (P = .011 and P = .011, respectively) and disease progression (P = .038 and P = .049, respectively) on univariate analysis and with need for surgery on multivariate analysis (P = .016 and P = .004, respectively). In the absence of the IBD5 risk haplotype, no association of OCTN1/2 variants with CD was detected. No associations were seen with UC. Conclusions: The IBD5 locus influences susceptibility, progression, and need for surgery in CD. However, the contribution of OCTN1/2 variants is not independent of the IBD5 haplotype; a causative role for these genes remains plausible but is not yet proven. Further genetic, functional, and expression data are now required. </p

Estimating the Economic Value of Australian Stock Herding Dogs

This study aimed to estimate the value of the typical Australian herding dog in terms of predicted return on investment. This required an assessment of the costs associated with owning herding dogs and estimation of the work they typically perform. Data on a total of 4,027 dogs were acquired through The Farm Dog Survey which gathered information from 812 herding dog owners around Australia. The median cost involved in owning a herding dog was estimated to be a total of AU$7,763 over the period of its working life. The work performed by the dog throughout this time was estimated to have a median value of AU$40,000. So, herding dogs typically provided their owners with a 5.2-fold return on investment. When respondents were asked to nominate the maximal, one-off, veterinary expenditure they would consider to remedy an illness or injury for an especially valued dog, the median response was AU$1,001–2,000 which is not concordant with the dogs’ calculated median lifetime value. The current findings equip working dog owners with useful information to make financially appropriate expenditure decisions related to their working dogs. This is expected to increase farm profitability and improve welfare for farm dogs

Valuable Behavioural Phenotypes in Australian Farm Dogs

This report presents findings regarding the ways to optimise the performance of livestock working dogs in Australia. The methods available to optimise dog performance include both environmental and genetic factors. The objectives of this study were to identify objective measures of desirable behavioural phenotypes in Australian livestock working dogs and explore genetic parameters of these \u27quality of working life\u27 traits within the Kelpie breed. The document reports on genotyping that reveals gene-behaviour associations and describes heritability estimates that underpin a database for potential estimated breeding value (EBV) calculations

Valuable Behavioural Phenotypes in Australian Farm Dogs

This report presents findings regarding the ways to optimise the performance of livestock working dogs in Australia. The methods available to optimise dog performance include both environmental and genetic factors. The objectives of this study were to identify objective measures of desirable behavioural phenotypes in Australian livestock working dogs and explore genetic parameters of these \u27quality of working life\u27 traits within the Kelpie breed. The document reports on genotyping that reveals gene-behaviour associations and describes heritability estimates that underpin a database for potential estimated breeding value (EBV) calculations

Estimating the Economic Value of Australian Stock Herding Dogs

This study aimed to estimate the value of the typical Australian herding dog in terms of predicted return on investment. This required an assessment of the costs associated with owning herding dogs and estimation of the work they typically perform. Data on a total of 4,027 dogs were acquired through The Farm Dog Survey which gathered information from 812 herding dog owners around Australia. The median cost involved in owning a herding dog was estimated to be a total of AU$7,763 over the period of its working life. The work performed by the dog throughout this time was estimated to have a median value of AU$40,000. So, herding dogs typically provided their owners with a 5.2-fold return on investment. When respondents were asked to nominate the maximal, one-off, veterinary expenditure they would consider to remedy an illness or injury for an especially valued dog, the median response was AU$1,001–2,000 which is not concordant with the dogs’ calculated median lifetime value. The current findings equip working dog owners with useful information to make financially appropriate expenditure decisions related to their working dogs. This is expected to increase farm profitability and improve welfare for farm dogs

Integrating personality research and animal contest theory: aggressiveness in the green swordtail <i>Xiphophorus helleri</i>

&lt;p&gt;Aggression occurs when individuals compete over limiting resources. While theoretical studies have long placed a strong emphasis on context-specificity of aggression, there is increasing recognition that consistent behavioural differences exist among individuals, and that aggressiveness may be an important component of individual personality. Though empirical studies tend to focus on one aspect or the other, we suggest there is merit in modelling both within-and among-individual variation in agonistic behaviour simultaneously. Here, we demonstrate how this can be achieved using multivariate linear mixed effect models. Using data from repeated mirror trials and dyadic interactions of male green swordtails, &lt;i&gt;Xiphophorus helleri&lt;/i&gt;, we show repeatable components of (co)variation in a suite of agonistic behaviour that is broadly consistent with a major axis of variation in aggressiveness. We also show that observed focal behaviour is dependent on opponent effects, which can themselves be repeatable but were more generally found to be context specific. In particular, our models show that within-individual variation in agonistic behaviour is explained, at least in part, by the relative size of a live opponent as predicted by contest theory. Finally, we suggest several additional applications of the multivariate models demonstrated here. These include testing the recently queried functional equivalence of alternative experimental approaches, (e. g., mirror trials, dyadic interaction tests) for assaying individual aggressiveness.&lt;/p&gt

A “Learning Revolution”? Investigating Pedagogic Practices around Interactive Whiteboards in British Primary Classrooms

Interactive whiteboards have been rapidly introduced into all primary schools under UK Government initiatives. These large, touch-sensitive screens, which control a computer connected to a digital projector, seem to be the first type of educational technology particularly suited for whole-class teaching and learning. Strong claims are made for their value by manufacturers and policy makers, but there has been little research on how, if at all, they influence established pedagogic practices, communicative processes and educational goals. This study has been designed to examine this issue, using observations in primary (elementary) school classrooms. It is funded by the UK Economic and Social Research Council and builds on the authors’ previous research on ICT in educational dialogues and collaborative activities

Electronic cigarettes: what we know so far

This paper summarises evidence relating to key issues surrounding e-cigarettes. It will be updated as new information emerges. Updated versions are made available on www.smokinginengland.info