492 research outputs found

    This doorknob is on the ceiling

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    This Doorknob is on the Ceiling is a thesis exhibition of sculptural works that explore the fusion of various domestic, industrial and commonplace objects. Some are new, but most of them are used and outdated. I explore and take advantage of the baggage that comes along with them, their history, their functions and what they suggest. My intention with these items is to both exploit and subvert the contextual boundaries which define them. I manipulate these objects in opposition to the way they are normally presented, encountered and understood, while leaving them recognizable. I am interested in obstructing function and creating unexpected associations between ordinary things. These works investigate dynamic spatial arrangements and codependent evocative relationships between objects that typically need human interaction to become activated or purposeful. I force them to depend on one another for physical support by intersecting and embedding them, creating animated arrangements motivated by indiscernible forces and circumstances

    Characterization of alloimmune responses and the generation of transplantation tolerance in thymectomized, thymus-implanted xenopus

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    This Thesis investigates the role of the Xenopus thymus in educating T cells to destroy minor histocompatibility (H) antigen-disparate skin grafts and probes the extent to which allotolerance is established to major histocompatibility complex (MHC) antigens first encountered at metamorphosis. The work began with intrastrain skin grafting and mixed leucocyte culture (MLC) (Chapter 2) which confirmed that only minor H antigen differences exist between J strain Xenopus laevis individuals, thus making them suitable as an additional animal model to the X.laevis/X.gilli (LG) isogeneic hybrids available. Chapter 3 revealed that rejection of minor H disparate grafts was completely thymus-dependent, while 7-day thymectomy (Tx) severely impaired rejection of MHC disparate grafts. Implantation (at 4-6 weeks of age) of larval or adult thymus from MHC-compatible or MHC-disparate donors restored the ability of Tx Xenopus to reject 3rd-party MHC antigen-disparate grafts, while tolerance to skin of the thymus donor type always ensued. Restoration of minor H graft rejection was impaired when thymus donor and TX host were MHC- or minor H antigen-mismatched. The use of LG hybrids revealed that minor H graft rejection was restored in Tx hosts only by implantation of a fully identical thymus. In Chapter 4, skin graft tolerance to donor MHC antigens was demonstrated following perimetamorphic allografting of skin to control J and LG recipients; the skin graft tolerance induced in certain donor/host combinations was shown to be not entirely specific for minor H antigens. In Chapter 5, in vitro, 1-way MLC reactivity of splenocytes from thymus-implanted Tx animals to thymus donor-type cells was shown to be variable, but occasionally positive; control Xenopus, made allotolerant of skin grafts by prior skin implantation retained a splenic MLC towards skin donor strain splenocytes. In vivo MLR assays in Chapter 6 also detected proliferation towards alloantigens of the skin or thymus donor, but the nature of these alloreactive (T-dependent) cells remains uncertain. Preliminary graft-versus-host experiments indicated that the tolerance induced by allothymus restoration of Tx hosts was more complete than following skin alloimplantation to metamorphosing controls; these studies also suggested that it is the cytotoxic effector component of tolerant animals that is defective

    Land uses that require a central business district location

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    M.S.Malcolm G. Little, Jr

    Hyperfine structure in the rotational spectrum of asymmetric-top molecules containing two identical quadrupolar nuclei

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    Ph.D.J. Q. Williams and T. L. Weatherl
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