Phase Behavior of Aqueous Na-K-Mg-Ca-CI-NO3 Mixtures: Isopiestic Measurements and Thermodynamic Modeling

A comprehensive model has been established for calculating thermodynamic properties of multicomponent aqueous systems containing the Na{sup +}, K{sup +}, Mg{sup 2+}, Ca{sup 2+}, Cl{sup -}, and NO{sub 3}{sup -} ions. The thermodynamic framework is based on a previously developed model for mixed-solvent electrolyte solutions. The framework has been designed to reproduce the properties of salt solutions at temperatures ranging from the freezing point to 300 C and concentrations ranging from infinite dilution to the fused salt limit. The model has been parameterized using a combination of an extensive literature database and new isopiestic measurements for thirteen salt mixtures at 140 C. The measurements have been performed using Oak Ridge National Laboratory's (ORNL) previously designed gravimetric isopiestic apparatus, which makes it possible to detect solid phase precipitation. Water activities are reported for mixtures with a fixed ratio of salts as a function of the total apparent salt mole fraction. The isopiestic measurements reported here simultaneously reflect two fundamental properties of the system, i.e., the activity of water as a function of solution concentration and the occurrence of solid-liquid transitions. The thermodynamic model accurately reproduces the new isopiestic data as well as literature data for binary, ternary and higher-order subsystems. Because of its high accuracy in calculating vapor-liquid and solid-liquid equilibria, the model is suitable for studying deliquescence behavior of multicomponent salt systems

Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol

The stilbene resveratrol is a stress metabolite produced by Vitis vinifera grapevines during fungal infection, wounding or UV radiation. Resveratrol is synthesised particularly in the skins of grape berries and only trace amounts are present in the fruit flesh. Red wine contains a much higher resveratrol concentration than white wine, due to skin contact during fermentation. Apart from its antifungal characteristics, resveratrol has also been shown to have cancer chemopreventive activity and to reduce the risk of coronary heart disease. It acts as an antioxidant and anti-mutagen and has the ability to induce specific enzymes that metabolise carcinogenic substances. The objective of this pilot study was to investigate the feasibility of developing wine yeasts with the ability to produce resveratrol during fermentation in both red and white wines, thereby increasing the wholesomeness of the product. To achieve this goal, the phenylpropanoid pathway in Saccharomyces cerevisiae would have to be introduced to produce p-coumaroyl-CoA, one of the substrates required for resveratrol synthesis. The other substrate for resveratrol synthase, malonyl-CoA, is already found in yeast and is involved in de novo fatty-acid biosynthesis. We hypothesised that production of p-coumaroyl-CoA and resveratrol can be achieved by co-expressing the coenzyme-A ligase-encoding gene (4CL216) from a hybrid poplar and the grapevine resveratrol synthase gene (vst1) in laboratory strains of S. cerevisiae. This yeast has the ability to metabolise p-coumaric acid, a substance already present in grape must. This compound was therefore added to the synthetic media used for the growth of laboratory cultures. Transformants expressing both the 4CL216 and vst1 genes were obtained and tested for production of resveratrol. Following β-glucosidase treatment of organic extracts for removal of glucose moieties that are typically bound to resveratrol, the results showed that the yeast transformants had produced the resveratrol β-glucoside, piceid. This is the first report of the reconstruction of a biochemical pathway in a heterologous host to produce resveratrol. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.Articl

Effects of food and fire on the demography of a nectar-feeding marsupial: A field experiment

Animals that specialize on nectar can be expected to face shortages of energy at times or in places where floral resources fade. Using a nectar-feeding marsupial, the eastern pygmy-possum Cercartetus nanus as a model, we predicted that animals would respond rapidly to an artificial energy supplement by showing improved body condition and increased local abundance. We also tested the hypothesis that responses would be more pronounced in burnt than in unburnt habitats due to the expectation that food would be limiting after fire. Energy was added in the form of sugar solution to two burnt and two unburnt woodland sites on the New South Wales coast, but not to two sets of equivalent control sites. The responses of pygmy-possums were compared between sites a month before and a month after supplementation during early autumn when flowers were diminishing. The energy supplement increased on-site immigration and a tail-fat index of body condition, but had no overall effect on the abundance or mean body mass of pygmy-possums. There was no effect of habitat; floral abundance was equal across sites. The results show that C. nanus responded rapidly to the energy supplement, and indicate that animals monitor shifts in the resource base continuously. Similar resource tracking abilities have been demonstrated previously in more mobile nectar-feeders, such as honeyeaters and bats. We suggest that such abilities should be generally advantageous in any species that depend on ephemeral resources

Comportamento ingestivo de novilhos de corte em pastagem de aveia preta e azevém com níveis distintos de folhas verdes Ingestive behaviour of beef steers grazing oat and Italian ryegrass pasture with different levels of green leaves

O objetivo deste trabalho foi avaliar como dois níveis distintos de biomassa de folhas verdes, representados por 350 e 600kg ha-1 de matéria seca de folhas verdes (MSFV) afetam o comportamento de novilhos de corte e sua taxa de bocados. Para as avaliações de comportamento ingestivo, foi utilizado o método direto de observação visual de animais focais em dois períodos de 24 horas, com início e término às 13:00 horas, durante o ciclo de duração da pastagem, nos dias 16-17/08 e 24-25/09/2002. Para cada turno de seis horas, foram utilizados três observadores treinados, um para cada dois potreiros contíguos (três animais focais por potreiro). A intervalos de 10 minutos eram registrados as atividades de pastejo, ruminação, ócio e realizadas medidas relativas à taxa de bocados, correspondente ao número de bocados de apreensão por minuto. A menor porcentagem de matéria seca existente na pastagem durante a primeira avaliação do comportamento ingestivo foi responsável pelo aumento no tempo de pastejo, na comparação entre períodos. A atividade complementar ao pastejo, na primeira avaliação, foi a ruminação, sem diferenças para ócio. As mais baixas biomassas induziram os animais a aumentar o número de bocados como forma de otimizar o consumo de forragem.<br>The objective of this trial was to evaluate how two levels of green leaves biomass, represented by 350 and 600kg ha-1 of green leaves dry matter (GLDM) affected beef steers behaviour and its bite rate. Evaluations of ingestive behaviour were performed with focal animals observed visually on two periods of 24 hours, with beginning and end at 01 pm, during pasture growth season, at 08/16-17 and 09/24-25/2002. Three trained observers were used for each turn of six hours, one for each two contiguous paddocks (where three focal animals grazed). At 10 minutes intervals, grazing time, rumination activities and rest were registered and estimated the bite rate, as the number of apprehension bites per minute. The lower dry matter percentage existing in the pasture during the first evaluation was responsible for the higher grazing time, when comparing the periods. In the first evaluation, the activity complementary to grazing was rumination and there was not differences for rest time. The lower biomass treatment induced the steers to take more bites as a way of optimizing forage consumption

Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol

The stilbene resveratrol is a stress metabolite produced by Vitis vinifera grapevines during fungal infection, wounding or UV radiation. Resveratrol is synthesised particularly in the skins of grape berries and only trace amounts are present in the fruit flesh. Red wine contains a much higher resveratrol concentration than white wine, due to skin contact during fermentation. Apart from its antifungal characteristics, resveratrol has also been shown to have cancer chemopreventive activity and to reduce the risk of coronary heart disease. It acts as an antioxidant and anti-mutagen and has the ability to induce specific enzymes that metabolise carcinogenic substances. The objective of this pilot study was to investigate the feasibility of developing wine yeasts with the ability to produce resveratrol during fermentation in both red and white wines, thereby increasing the wholesomeness of the product. To achieve this goal, the phenylpropanoid pathway in Saccharomyces cerevisiae would have to be introduced to produce p-coumaroyl-CoA, one of the substrates required for resveratrol synthesis. The other substrate for resveratrol synthase, malonyl-CoA, is already found in yeast and is involved in de novo fatty-acid biosynthesis. We hypothesised that production of p-coumaroyl-CoA and resveratrol can be achieved by co-expressing the coenzyme-A ligase-encoding gene (4CL216) from a hybrid poplar and the grapevine resveratrol synthase gene (vst1) in laboratory strains of S. cerevisiae. This yeast has the ability to metabolise p-coumaric acid, a substance already present in grape must. This compound was therefore added to the synthetic media used for the growth of laboratory cultures. Transformants expressing both the 4CL216 and vst1 genes were obtained and tested for production of resveratrol. Following ?-glucosidase treatment of organic extracts for removal of glucose moieties that are typically bound to resveratrol, the results showed that the yeast transformants had produced the resveratrol β-glucoside, piceid. This is the first report of the reconstruction of a biochemical pathway in a heterologous host to produce resveratrol.7 page(s