93 research outputs found
Tecidos vegetais.
Amostragem; Procedimento para coleta de amostras de folhas no campo; Coleta da amostra; Identificacao da amostra; Moagem; Armazenagem; Integridade da amostra; Problemas de contaminacao; Arquivo de amostra
Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation
The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts
Common Promoter Elements in Odorant and Vomeronasal Receptor Genes
In mammals, odorants and pheromones are detected by hundreds of odorant receptors (ORs) and vomeronasal receptors (V1Rs and V2Rs) expressed by sensory neurons that are respectively located in the main olfactory epithelium and in the vomeronasal organ. Even though these two olfactory systems are functionally and anatomically separate, their sensory neurons show a common mechanism of receptor gene regulation: each neuron expresses a single receptor gene from a single allele. The mechanisms underlying OR and VR gene expression remain unclear. Here we investigated if OR and V1R genes share common sequences in their promoter regions
Avaliação do concreto reforçado com baixos teores de fibras para fins estruturais segundo a norma EN 14651-2007
Targeting cancer cell death with a bcl-x S adenovirus
Transformation is a complex cellular process that requires several genetic abnormalities. In many cases, one of these abnormalities is an inhibition of PCD, which provides a selective advantage for tumor cells. This has been recently shown in an in vivo model, where overexpression of Bcl-x L is a crucial step in the progression from hyperplasia to neoplasia and is accompanied by a significant decrease in tumor apoptosis [56].Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46936/1/281_2004_Article_BF00787225.pd
TURNOVER, CHANGE of COMPOSITION WITH RATE of CELL-GROWTH and EFFECT of PHENYLXYLOSIDE ON SYNTHESIS and STRUCTURE of CELL-SURFACE SULFATED GLYCOSAMINOGLYCANS of NORMAL and TRANSFORMED-CELLS
ESCOLA PAULISTA MED,DEPT BIOQUIM,CX POSTAL 20372,São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT BIOQUIM,CX POSTAL 20372,São Paulo,SP,BRAZILWeb of Scienc
Transcription and processing of ribonucleic acid in Rhynchosciara salivary glands. I. Rapidly labeled ribonucleic acid
Citius, altius, fortius/faster, higher, tougher: pushing ahead the boundaries of structural concrete through fiber-reinforced cementitious composites with adapted rheology
Detection and Measurement of Experimentally Produced Aortic Regurgitation by Means of Indicator-Dilution Curves Recorded from the Left Ventricle
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