Super yatch design study for Malaysian sea (Langkawi Island)

Malaysia as a country surrounded by water has a huge economic and geographical potential in the development of super yacht industry in South East Asia. There is lack of super yacht design study specifying to Malaysian marinas and seas. Most of the super yacht operates in Malaysia were built and bought directly from oversea, and chartered by foreign companies. It is hence the purpose of this study to survey on Malaysian sea water, particularly Langkawi Island, to introduce a design methodology in producing a preliminary design of super yacht that suits Langkawi Island, and serves as a guideline for future super yacht design for Malaysian sea in different marinas. Suitable dimensions of super yacht were derived by using dimensional relationship via statistical method. Two types of hull form designs (round bilge and V-bottom hull) were designed using Maxsurf Pro software. Resistance analysis on the two hull forms were carried out using Savitsky Pre-Planing and Compton methods via MaxsurfHullspeed software, and stability performance of the two hull forms was analyze using Hydromax software. VBottom hull form is found to have better resistance performance as compared to round bilge hull form, and both hull forms are found to be in stable conditions and comply with IMO requirements

Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions

BackgroundTargeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing.ResultsAll panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden.ConclusionThis comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.Peer reviewe

Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo

Age diversity and learning outcomes in organizational training groups: the role of knowledge sharing and psychological safety

This study advances theorizing on human resource development by conceptualizing a training group’s age diversity composition as an antecedent of participants’ learning outcomes in organizational training courses. Drawing from social identity theory, we propose that a high age diversity of the training group can inhibit participants’ learning outcomes because individuals are less likely to share knowledge than in age-homogenous groups. Furthermore, we expect that psychological safety serves as a buffer, such that participants who perceive a high level of psychological safety in a training group will engage in knowledge sharing and consequently report high learning outcomes, regardless of the training group’s age diversity composition. We tested the proposed moderated mediation model in a sample of 211 employees participating in an interactive oneday training at an automobile manufacturer. We found that perceived age diversity, but not objective age diversity, was negatively linked to participants’ learning outcomes and that this relationship was mediated by knowledge sharing. Participants’ perceptions of psychological safety served as a buffer against the negative effect of perceived age diversity. We discuss implications for the conceptual understanding of learning as an active process shaped by the training group and encourage scholars to broaden their understanding of training design elements

The role of religious leaders in promoting acceptance of vaccination within a minority group: a qualitative study

Contains fulltext : 117840.pdf (publisher's version ) (Open Access)BACKGROUND: Although childhood vaccination programs have been very successful, vaccination coverage in minority groups may be considerably lower than in the general population. In order to increase vaccination coverage in such minority groups involvement of faith-based organizations and religious leaders has been advocated. We assessed the role of religious leaders in promoting acceptance or refusal of vaccination within an orthodox Protestant minority group with low vaccination coverage in The Netherlands. METHODS: Semi-structured interviews were conducted with orthodox Protestant religious leaders from various denominations, who were selected via purposeful sampling. Transcripts of the interviews were thematically analyzed, and emerging concepts were assessed for consistency using the constant comparative method from grounded theory. RESULTS: Data saturation was reached after 12 interviews. Three subgroups of religious leaders stood out: those who fully accepted vaccination and did not address the subject, those who had religious objections to vaccination but focused on a deliberate choice, and those who had religious objections to vaccination and preached against vaccination. The various approaches of the religious leaders seemed to be determined by the acceptance of vaccination in their congregation as well as by their personal point of view. All religious leaders emphasized the importance of voluntary vaccination programs and religious exemptions from vaccination requirements. In case of an epidemic of a vaccine preventable disease, they would appreciate a dialogue with the authorities. However, they were not willing to promote vaccination on behalf of authorities. CONCLUSION: Religious leaders' attitudes towards vaccination vary from full acceptance to clear refusal. According to orthodox Protestant church order, local congregation members appoint their religious leaders themselves. Obviously they choose leaders whose views are compatible with the views of the congregation members. Moreover, the positions of orthodox Protestant religious leaders on vaccination will not change easily, as their objections to vaccination are rooted in religious doctrine and they owe their authority to their interpretation and application of this doctrine. Although the dialogue with religious leaders that is pursued by the Dutch government may be helpful in controlling epidemics by other means than vaccination, it is unlikely to increase vaccination coverage

Smoke extracts and nicotine, but not tobacco extracts, potentiate firing and burst activity of Ventral Tegmental Area dopaminergic neurons in mice

International audienceNicotine prominently mediates the behavioral effects of tobacco consumption, either through smoking or when taking tobacco by snuff or chew. However, many studies question the exclusive role of nicotine in these effects. The use of preparations containing all the components of tobacco, such as tobacco and smoke extracts, may be more suitable than nicotine alone to investigate the behavioral effects of smoking and tobacco intake. In the present study, the electrophysiological effects of tobacco and smoke on ventral tegmental area dopamine (DA) neurons were examined in vivo in anesthetized wild-type (WT), ß2 nicotinic acetylcholine receptor (nAChR) knock-out (ß2-/-), α4-/- and α6-/- mice and compared to those of nicotine alone. In WT mice, smoke and nicotine had similar potentiating effects on DA cells activity, but the action of tobacco on neuronal firing was weak, and often inhibitory. In particular, nicotine triggered strong bursting activity, while no bursting activity was observed after tobacco extract administration. In ß2-/- mice, nicotine or extract elicited no modification of the firing patterns of DA cells, indicating that extract act predominantly through nAChRs. The differences between DA cells activation profiles induced by tobacco and nicotine alone observed in WT persited in α6-/- mice but not in α4-/- mice. These results would suggest that tobacco has lower addiction generating properties compared to either nicotine alone or smoke. The weak activation and prominent inhibition obtained with tobacco extracts suggest that tobacco contains compounds which counteract some of the activating effects of nicotine and promote inhibition on DA cell acting through α4ß2*-nAChRs. The nature of these compounds remains to be elucidated. It nevertheless confirms that nicotine is the main substance involved in the tobacco addiction-related activation of mesolimbic DA neurons