The effect of aerobic exercise after exposure to black carbon particulate matter 2.5 on expression of NF-κB and TNF-α genes in the lung tissue of male rats

Background: The aim of the present study was to investigate the effect of aerobic exercise after exposure to black carbon particulate matter 2.5 (PM 2.5) on expression of NF-κB, and TNF-α genes in the lung tissue of male rats. Materials and Methods: Twenty-four male Wistar rats (mean weight, 279.29±26.97 g) were randomly divided into four equal groups: 1) control (without any intervention), 2) without aerobic exercise and with exposure to PM2.5 black carbon, 3) aerobic exercise without exposure to black carbon, and 4) aerobic exercise after exposure to PM2.5 black carbon. The aerobic exercise protocol was performed at 50 the maximal speed of each group for 4 weeks 15 minutes after exposure to black carbon particles in the chamber. Animals were sacrificed 24 hours after the last training session and exposure to black carbon particles. The expression of NF-κB and TNF-α genes were analyzed in the lung tissue of rats by the real-time PCR. Results: A significant difference was found in the NF-κB expression between the group 1 and other groups and also there was a significant difference in the TNF-α expression between the group 2 and group 3 after exposure to black carbon in the lung tissue of male rats. Conclusion: The findings show that aerobic exercise after exposure to the black carbon particles (PM2.5) is associated with adjustment in lung inflammatory factors. It seems that these changes in part are associated with a lower risk of inflammatory pulmonary disease

Potential Improvement in Rehabilitation Quality of 2019 Novel Coronavirus by Isometric Training System; Is There “Muscle-Lung Cross-Talk”?

The novel Coronavirus Disease 2019 (COVID-19) crisis is now present in more than 200 countries. It started in December 2019 and has, so far, led to more than 149, 470,968 cases, 3,152,121 deaths, and 127,133,013 survivors recovered by 28 April 2021. COVID-19 has a high morbidity, and mortality of 2%, on average, whereas most people are treated after a period of time. Some people who recover from COVID-19 are left with 20 to 30% decreased lung function. In this context, exercise focused on skeletal muscle with minimal lung involvement could potentially play an important role. Regular exercise protects against diseases associated with chronic low-grade systemic inflammation. This long-term effect of exercise may be ascribed to the anti-inflammatory response elicited by an acute bout of exercise, which is partly mediated by muscle-derived myokines. The isometric training system seems to have this feature, because this system is involved with the skeletal muscle as the target tissue. However, no studies have examined the effect of exercise on the treatment and recovery of COVID-19, and, more importantly, "muscle-lung cross-talk" as a mechanism for COVID-19 treatment. It is suggested that this theoretical construct be examined by researchers

Prevalence of blaCTX-M Gene among Extended-Spectrum β-Lactamases Producing Klebsiellapneumoniae Clinical Isolates in Iran: A Meta-Analysis

Background: CTX-M-type extended-spectrum β-lactamases (ESBLs) are the most prevalent ESBLs in bacterial members of Enterobacteriaceaefamily including Klebsiella pneumoniae. The global spread of CTX-M-producing K. pneumoniae is a major concern in most countries including Iran. The aim of this meta-analysis was to determine the relative frequency (RF) of blaCTX-M gene among ESBLs-producing K. pneumoniae clinical isolates in Iran and to report an overall prevalence. Methods: A comprehensive literature search of studies published up to July 2016 was carried out. The keywords “Enterobacteriaceae”, “Klebsiella pneumoniae”, “ESBLs”, “CTX-M” and “Iran” were searched in PubMed, Scopus, EBSCO, Google Scholar, Scirus, SID and IranMedex in both English and Persian. Selected articles were published between July 2010 and July 2016 and all of them were in English. STATA SE version 11.0 was used for statistical analysis. Results: Twenty-four articles/abstracts were included in this analysis. Selected studies were performed in Ahvaz, Arak, Ilam, Kashan, Kerman, Mashhad, Shiraz, Tabriz, Tehran, Zabol, and Zahedan. Our pooled evidence showed that the RF of blaCTX-M gene among ESBLs-producing K. pneumoniae clinical isolates varied from 7.7% in Tabriz to 100% in Mashhad, Tehran, and Zahedan, with an overall RF of 56.7%. Conclusion: Our meta-analysis revealed that the RF of CTX-M-type ESBLs-producing K. pneumoniae is diverse in different regions of Iran, and the central and eastern regions had higher prevalence rates compared to western regions

Resistance exercise in a hot environment alters serum markers in untrained males

Purpose:We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods:Ten untrained young males (26 +/- 3 years; 75.8 +/- 6 kg; 177.4 +/- 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30-60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (similar to 35 degrees C) and thermoneutral (22 degrees C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results:There were time-related changes in testosterone, HSP70, and IL-6 (P 0.05). Conclusion:RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory markers

Resistance Exercise in a Hot Environment Alters Serum Markers in Untrained Males

[EN] Purpose: We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods: Ten untrained young males (26 ± 3 years; 75.8 ± 6 kg; 177.4 ± 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30–60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (∼35◦C) and thermoneutral (22◦C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results: There were time-related changes in testosterone, HSP70, and IL-6 (P < 0.001), and cortisol and IL-15 (P < 0.05). Levels of cortisol, HSP70, and IL- 6 increased immediately for RE at 35◦C, and testosterone and IL-15 levels were decreased. Changes in serum testosterone, HSP70, cortisol, and IL-15 and IL-6 levels were reversed after 1 h. A significant time × condition interaction was observed for IL-15 and HSP70 (P 0.05). Conclusion: RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory marker

Design and psychometrics of risk assessment tool for makeshift hospitals: Focusing on pandemics

Pandemics caused a change in the health service delivery system. In disasters with massive injuries or epidemic, the capacity to admit the injured and infected patients to provide health services faces the challenges. Covid-19 virus is a respiratory disease that from its emergence until January 18, 2023, the Covid-19 virus has infected more than 672 million people worldwide. In most countries, makeshift hospital has been set up as intermediate medical centers to keep people who are likely to be carriers of the disease to control communicable diseases. Most makeshift hospitals already have alternative uses and, have changed into temporary or intermediate care centers. Based on the search of research team, any standard tools were found to assess the makeshift hospitals safety. All the safety assessment tools were related to the hospital, but since makeshift hospitals are mostly non-hospital structures, the research team has designed and validate a makeshift hospital safety assessment tool for the first time in this study.The present study is a mixed method that was conducted in 3 phases including; a document review, explaining the components affecting non-structural and functional safety of the makeshift hospital, designing makeshift hospital safety assessment tools and analyzing the results and validating it in 2022. Content validity and reliability were measured by CVR and CVI, ICC and Cronbach's alpha. In the fourth phase, with the participation of 15 specialists, managers, experts, qualitative and quantitative validity of content was done. Data were analyzed by SPSS version 21 software.The final tool contains 186 items and a 5-point Likert designed for very low safety (1), low safety (2), moderate safety (3), good safety (4), and very good safety (5). The scores of each makeshift hospital were calculated based on the items and the degree of safety. Cronbach's alpha coefficient for tool was 0.98. Retesting the questionnaire after two weeks confirmed the stability of tool (ICC = 0.98). The validity and reliability of this tool were confirmed with 186 items in 2 factors and 26 subcategories including risk of disaster, non-structural safety, safety of windows and shutters, hospital access, information and communication management, patient safety and hygiene and etc.All centers providing health services, whether temporarily or permanently, must have safety to continue their activities in disaster and maintain the safety and health of staff and inpatients. The makeshift hospital safety tool can be a suitable tool for assessing the risk and eliminating their vulnerabilities, and it can also provide important indicators for the design and set up of the makeshift hospital to policymakers and executives in the field of health

The effect of aerobic exercise after exposure to black carbon particulate matter 2.5 on expression of NF-κB and TNF-α genes in the lung tissue of male rats

Background: The aim of the present study was to investigate the effect of aerobic exercise after exposure to black carbon particulate matter 2.5 (PM 2.5) on expression of NF-κB, and TNF-α genes in the lung tissue of male rats. Materials and Methods: Twenty-four male Wistar rats (mean weight, 279.29±26.97 g) were randomly divided into four equal groups: 1) control (without any intervention), 2) without aerobic exercise and with exposure to PM2.5 black carbon, 3) aerobic exercise without exposure to black carbon, and 4) aerobic exercise after exposure to PM2.5 black carbon. The aerobic exercise protocol was performed at 50% the maximal speed of each group for 4 weeks 15 minutes after exposure to black carbon particles in the chamber. Animals were sacrificed 24 hours after the last training session and exposure to black carbon particles. The expression of NF-κB and TNF-α genes were analyzed in the lung tissue of rats by the real-time PCR. Results: A significant difference was found in the NF-κB expression between the group 1 and other groups and also there was a significant difference in the TNF-α expression between the group 2 and group 3 after exposure to black carbon in the lung tissue of male rats. Conclusion: The findings show that aerobic exercise after exposure to the black carbon particles (PM2.5) is associated with adjustment in lung inflammatory factors. It seems that these changes in part are associated with a lower risk of inflammatory pulmonary disease