Departamento de Mejora Genética Vegetal Grupo de Investigación en Biotecnología Vegetal

Comprender el desarrollo de un sistema de Androesterilidad en Trigo para el Mejoramiento genético de la especieCONACYT - Consejo Nacional de Ciencia y TecnologíaPROCIENCI

Modulating climacteric intensity in melon through QTL stacking

Fruit ripening is one of the main processes affecting fruit quality and shelf life. In melon there are both climacteric and non-climacteric genotypes, making it a suitable species to study fruit ripening. In the current study, in order to fine tune ripening, we have pyramided three climacteric QTLs in the non-climacteric genotype “Piel de Sapo”: ETHQB3.5, ETHQV6.3 and ETHQV8.1. The results showed that the three QTLs interact epistatically, affecting ethylene production and ripening-related traits such as aroma profile. Each individual QTL has a specific role in the ethylene production profile. ETHQB3.5 accelerates the ethylene peak, ETHQV6.3 advances the ethylene production and ETHQV8.1 enhances the effect of the other two QTLs. Regarding aroma, the three QTLs independently activated the production of esters changing the aroma profile of the fruits, with no significant effects in fruit firmness, soluble solid content and fruit size. Understanding the interaction and the effect of different ripening QTLs offers a powerful knowledge for candidate gene identification as well as for melon breeding programs, where fruit ripening is one of the main objectives.info:eu-repo/semantics/publishedVersio

Evaluación in vitro de 4 aislados de Trichoderma sp. para el control de Macrophomina phaseolina (Tassi) Goid

El trabajo tiene los siguientes objetivos: - Evaluar 3 aislados nativos y 1 comercial de Trichoderma sp. para el control in vitro de M. phaseolina. - Determinar el crecimiento lineal, la velocidad media de crecimiento y el grado de sobreposición micelial.CONACYT – Consejo Nacional de Ciencia y TecnologíaPROCIENCI

Map-based cloning of two Quantitative Trait Loci for resistance to Cucumber mosaic virus in melon

Trabajo presentado en el Stress Seminar del Centre de Recerca Agrigenómica (CRAG), celebrado el 9 de marzo de 2021.Peer reviewe

Resistance to Cucumber mosaic virus (CMV) in Near Introgression Lines (NIL) containing two and three Quantitative Trait Loci (QTL) in melon

Resumen del póster presentado al XIIth Eucarpia Meeting on Cucurbit Genetics and Breeding, celebrado on-line del 24 al 28 de mayo de 2021.Cucumber mosaic virus (CMV) can infect more than 1.200 different plant species belonging to Solanaceae and Cucurbitaceae families, causing severe damage in leaves and strong fruit lesions. Resistance to CMV has been found in several exotic melon accessions, being one of them the Korean accession “Songwhan Charmi” (SC). A doubled haploid line (DHL) collection was previously developed from a cross between SC as resistant parental and the cultivar Piel de Sapo (PS) as a susceptible parental, in order to study the resistance trait. A Quantitative Trait Loci (QTL) analysis revealed a major QTL cmvqw12.1 located in the linkage group XII. This QTL confer total resistance to subgroup II strain LS. However, for the subgroup I strains M6 and FNY it is necessary but not sufficient. In addition, two more QTLs were described, cmvqw3.1 located in LGIII and cmvqw10.1 in LGX. DHLs containing the three QTLs were resistant to M6, whereas DHLs containing two QTLs, being one of them cmvqw12.1, were susceptible to M6 strain. In the present work we have developed Near Isogenic Lines (NILs) containing either two or three QTL. These are a NIL with cmvqw3.1 and cmvqw12.1, a NIL with cmvqw10.1 and cmvqw12.1 and a NIL with the three QTL. Our aim is to confirm in the NILs the resistance to CMV-M6 observed previously in the DHLs containing two and three QTLs, in order to know whether the results are consistent in these two different genetic backgrounds.Peer reviewe

Resistance to Cucumber mosaic virus (CMV) in melon Near Introgression Lines (NIL) carrying two or three Quantitative Trait Loci (QTL)

Trabajo presentado en la III Reunión de la Red Nacional de Virología de Plantas, RENAVIPLANT, celebrada en Murcia entre el 25 y el 27 de mayo de 2022.Cucumber mosaic virus (CMV) can infect more than 1.200 different plant species belonging to Solanaceae and Cucurbitaceae families. Resistance to CMV has been found in several exotic melon accessions, being one of them the Korean accession “Songwhan Charmi” (SC). A doubled haploid line (DHL) collection was previously developed from a cross between SC as resistant parental and the cultivar Piel de Sapo (PS) as a susceptible parental, in order to study the resistance trait. A Quantitative Trait Loci (QTL) analysis revealed a major QTL, cmvqw12.1, located in the linkage group XII. This QTL confers total resistance to subgroup II strain LS. However, for the subgroup I strains M6 and FNY it is necessary but not sufficient. In addition, two more QTLs were described, cmvqw3.1 located in LGIII and cmvqw10.1 in LGX. DHLs containing the three QTLs were resistant to M6, whereas DHLs containing two QTLs, being one of them cmvqw12.1, were susceptible to M6 strain. In the present work we have developed Near Isogenic Lines (NILs) containing either two or three QTL. These are a NIL with cmvqw3.1 and cmvqw12.1, a NIL with cmvqw10.1 and cmvqw12.1 and a NIL with the three QTL. Our aim is to confirm in the NILs the resistance to CMV-M6 observed previously in the DHLs containing two and three QTLs, in order to know whether the results are consistent in these two different genetic backgrounds. From these ILs we are generating mapping populations for both QTLs cmvqw3.1 and cmvqw10.1

Characterization of resistance to CMV Subgroup I strains in Introgression Lines (IL) containing two and three Quantitative Trait Loci (QTL) in melon

Póster presentado al XX Congreso Nacional de Fitopatología celebrado en Valencia entre el 24 y el 26 de octubre de 2022.Cucumber mosaic virus (CMV) can infect up to 1200 plant species including those with high economic importance such as Solanaceae and Cucurbitaceae families. The Korean melon accession “Songwhan Charmi” (SC)” is one of the CMV-resistant accessions identified to date, carrying an oligogenic and quantitative resistance. A Doubled Haploid Line (DHL) and a Introgression Lines (ILs) populations were developed from a cross between SC and Piel de Sapo (PS). A Quantitative Trait Loci (QTL) analysis in the DHL population revealed a major QTL, cmv1, located in the chromosome XII and conferring total resistance to the subgroup II strain LS but not to the subgroup I strains. cmv1 itself is able to restrict CMV-LS in the bundle sheath (BS) cells. Two additional QTLs, cmvqw3.1, mapping in the chromosome III, and cmvqw10.1, mapping in the chromosome X, are needed in coordination with cmv1 for resistance to SG I strains. In the present work we have developed ILs containing either two or three QTL. These are an IL with cmvqw3.1 and cmv1, an IL carrying cmvqw10.1 and cmv1 and an IL carrying the three QTL. These ILs do not stop CMV-FNY, but delay phloem entry in different extension. However, the ILs with two QTLs are susceptible to CMV-M6, whereas the IL carrying three QTL can stop CMV-M6 infection. Here we aim to characterize the resistance/susceptibility of these ILs to CMV-M6 by quantifying the virus present in the phloem of inoculated leaves