Nandrolone Administration Does Not Promote Hypertrophy Of Soleus Muscle In Rats [a Administração De Nandrolona Não Promove Hipertrofia Do Músculo Sóleo Em Ratos]

Anabolic androgenic steroids (AAS) are compounds formed from testosterone or one of its derivatives, which are largely used by amateur e professional athletes to improve the athletic performance. However, the scientific information about the relation between the use of AAS and muscle hypertrophy is controversial. The aim of this study was to evaluate the effects of testosterone and physical training on muscle hypertrophy. Male Wistar rats received i.m. injections of Deca-Durabolin® or vehicle during 6 weeks. Trained rats were submitted to a resistance physical training, by jumping up and down in water carrying an overload. Sedentary and trained animals were anesthetized and sacrificed. Soleus muscle was removed for the quantification of total protein and DNA concentration. In the end of the treatment, body weight of trained animals treated with vehicle or AAS was lower than the body weight of respective sedentary. Total protein concentration and the ratio muscle weight/body weight of all experimental groups were not altered. Trained group treated with AAS presented lower DNA concentration than trained group treated with vehicle. The administration of nandrolone decanoate did not promote hypertrophy on soleus muscle, not even when the use of AAS was associated to resistance physical training.503532540Kutscher, E.C., Lund, B.C., Perry, P.J., Anabolic steroids: A review for the clinician (2002) Sports Med, 32 (5), pp. 285-296Wilson, J.D., Androgen abuse by athletes (1988) Endocr Rev, 9 (2), pp. 181-199Creutzberg, E.C., Schols, A.M.W.J., Anabolic steroids (1999) Curr Opin Clin Nutr Metab Care, 2 (3), pp. 243-253Cunha, T.S., Cunha, N.S., Moura, M.J.C.S., Marcondes, F.K., Esteróides anabólicos androgênicos e sua relação com a prática desportiva (2004) Rev Bras Ciênc Farmac, 40 (2), pp. 165-179Johnson, W.O., Steroids: A problem of huge dimensions (1985) Sports Illustrated, 5 (13), pp. 38-54Yesalis, C.E., Courson, S.P., Wright, J.E., History of anabolic steroid in sport and exercise (1993) Anabolic Steroids in Sport and Exercise, pp. 51-71. , Yesalis CE, ed. Champaign:Human KineticsKuipers, H., Wijnen, J.A., Hartgens, F., Willems, S.M., Influence of anabolic steroids on body composition, blood pressure, lipid profile and liver functions in body builders (1991) Int J Sports Med, 12, pp. 413-418Gruber, A.J., Pope Jr., H.G., Psychiatric and medical effects of anabolic-androgenic steroid use in women (2000) Psychother Psychosom, 69 (1), pp. 19-26Bahrke, M.S., Yesalis, C.E., Kopstein, A.N., Stephens, J.A., Risk factors associated with anabolic-androgenic steroid use among adolescents (2000) Sports Med, 29 (6), pp. 397-405Van Breda, E., Keizer, H.A., Geurten, P., Van Kranenburg, G., Menheere, P.P., Kuipers, H., Modulation of glycogen metabolism of rat skeletal muscles by endurance training and testosterone treatment (1993) Pflugers Arch, 424 (3-4), pp. 294-300Cunha, T.S., Tanno, A.P., Moura, M.J.C.S., Marcondes, F.K., Effect of high intensity exercise and anabolic-androgenic steroid administration on liver and muscle glycogen supercompensation in rats (2003) XXI Congresso Latino Americano de Ciências Fisiológicas, p. 210. , Ribeirão PretoCunha, T.S., (2004) Efeito Do Esteróide Anabólico Androgênico Nandrolona Sobre O Metabolismo Do Glicogênio Em Ratos Sedentários e Treinados, , Piracicaba, Dissertação de Mestrado - Faculdade de Odontologia da Universidade Estadual de CampinasSilva, P.R.P., Danielski, R., Czepielewski, M.A., Esteróides anabolizantes no esporte (2002) Rev Bras Med Esporte, 8 (6), pp. 235-243Voltarelli, F.A., Gobatto, C.A., Mello, M.A.R., Determination of anaerobic threshold in rats using the lactate minimum test (2002) Braz J Med Biol Res, 35, pp. 1389-1394Tamaki, T., Uchiyama, S., Uchiyama, Y., Akatsuka, A., Yoshimura, S., Roy, R.R., Limited myogenic response to a single bout of weight-lifting exercise in old rats (2000) Am J Physiol Cell Physiol, 278 (6), pp. C1143-52Elashoff, J.D., Jacknow, A.D., Shain, S.G., Braunstein, G.D., Effects of anabolic-androgenic steroids on muscular strength (1991) Ann Intern Med, 115 (5), pp. 387-393Kuhn, C.M., Anabolic steroids (2002) Recent Prog Horm Res, 57, pp. 411-434Norton, G.R., Trifunovic, B., Woodiwiss, A.J., Attenuated beta-adrenoceptor-mediated cardiac contractile responses following androgenic steroid administration to sedentary rats (2000) Eur J Appl Physiol, 81 (4), pp. 310-316Pope Jr., H.G., Katz, D.L., Affective and psychotic symptoms associated with anabolic steroid use (1988) Am J Psychiatry, 145 (4), pp. 487-490Rogatto, G.P., (2001) Efeitos Do Treinamento Físico de Alta Intensidade Sobre Aspectos Endócrino-metabólicos de Ratos Wistar, , Rio Claro, Dissertação de Mestrado - Instituto de Biociências da Universidade Estadual PaulistaNakatani, A., Han, D., Hansen, P.A., Nolte, L.A., Host, H.H., Hickner, R.C., Effect of endurance exercise training on muscle glycogen supercompensation in rats (1997) J Appl Physiol, 82 (2), pp. 711-715Greiwe, J.S., Hickner, R.C., Hansen, P.A., Racette, S.B., Chen, M.M., Holloszy, J.O., Effects of endurance exercise training on muscle glycogen accumulation in humans (1999) J Appl Physiol, 87 (1), pp. 222-226Tanno, A.P., Bianchi, F.J., Moura, M.J.C.S., Marcondes, F.K., Atrial supersensitivity to noradrenaline in stressed female rats (2002) Life Sci, 71 (25), pp. 2973-2981Bradford, M., A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254Chomczynski, P., Mackey, K., Drews, R., Wilfinger, W., DNAzol: A reagent for the rapid isolation of genomic DNA (1997) BioTechniques, 22, pp. 550-553Ästrand, P.O., Why exercise? (1991) Med Sci Sports Exerc, 24, pp. 153-162Radak, Z., Kaneko, T., Tahara, S., Nakamoto, H., Ohno, H., Sasvari, M., The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: Evidence for beneficial outcomes (1999) Free Radic Biol Med, 27 (1-2), pp. 69-74Rikli, R.E., Reliability, validity, and methodological issues in assessing physical activity in older adults (2000) Res Q Exerc Sport, 71 (2), pp. 89-96Yoshioka, M., Doucet, E., St-Pierre, S., Almeras, N., Richard, D., Labrie, A., Impact of high-intensity exercise on energy expenditure, lipid oxidation and body fatness (2001) Int J Obes Relat Metab Disord, 25 (3), pp. 332-339Hunter, G.R., Weinsier, R.L., Bamman, M.M., Larson, D.E., A role for high intensity exercise on energy balance and weight control (1998) Int J Obes Relat Metab Disord, 22 (6), pp. 489-493Bauman, D.H., Richerson, J.T., Britt, A.L., A comparison of body and organ weights, physiologic parameters, and pathologic changes in target organs of rats given combinations of exercise, anabolic hormone, and protein supplementation (1988) Am J Sports Med, 16 (4), pp. 397-402Carson, J.A., Lee, W.J., Mcclung, J., Hand, G.A., Steroid receptor concentration in aged rat hind limb muscle: Effect of anabolic steroid administration (2002) J Appl Physiol, 93, pp. 242-250Hickson, R.C., Kurowski, T.G., Anabolic steroids and training (1986) Clin Sports Med, 5 (3), pp. 461-469Ryan, A.J., Anabolic steroids are fool's gold (1981) Fed Proc, 40 (12), pp. 2682-2688Guzman, M., Saborido, A., Castro, J., Molano, F., Megias, A., Treatment with anabolic steroids increases the activity of the mitochondrial outer carnitin palmitoyltransferase in rat liver and fast-twitch muscle (1991) Biochem Pharm, 41, pp. 833-835Tamaki, T., Uchiyama, S., Uchiyama, Y., Akatsuka, A., Roy, R.R., Edgerton, V.R., Anabolic steroids increase exercise tolerance (2001) Am J Physiol Endocrinol Metab, 280 (6), pp. 973-981Schultz, E., Jaryszak, D.L., Gibson, M.C., Albright, D.J., Absence of exogenous satellite cell contribution to regeneration of frozen skeletal muscle (1986) J Muscle Res Cell Motil, 7, pp. 361-367Hawke, T.J., Garry, D.J., Myogenic satellite cells: Physiology to molecular biology (2001) J Appl Physiol, 91 (2), pp. 534-551Phillips, S.M., Tipton, K.D., Ferrando, A.A., Wolfe, R.R., Resistance training reduces the acute exercise-induced increase in muscle protein turnover (1999) Am J Physiol, 276, pp. 118-124Yarasheski, K.E., Campbell, J.A., Smith, K., Rennie, M.J., Holloszy, J.O., Bier, D.M., Effect of growth hormone and resistance exercise on muscle growth in young men (1992) Am J Physiol, 262, pp. 261-267Yarasheski, K.E., Zachwieja, J.J., Bier, D., Acute effects of resistance exercise on muscle protein synthesis rate in young and elderly men and women (1993) Am J Physiol, 265, pp. 210-214Joubert, Y., Tobin, C., Satellite cell proliferation and increase in the number of myonuclei induced by testosterone in the levator ani muscle of the adult female rat (1989) Dev Biol, 131 (2), pp. 550-557Tamaki, T., Uchiyama, S., Nakano, S., A weight-lifting exercise model for inducing hypertrophy in the hind limb muscles of rats (1992) Med Sci Sports Exerc, 24 (8), pp. 881-886Tamaki, T., Akatsuka, A., Tokunaga, M., Ishige, K., Uchiyama, S., Shiraishi, T., Morphological and biochemical evidence of muscle hyperplasia following weight-lifting exercise in rats (1997) Am J Physiol, 273 (1 PART 1), pp. C246-56Sandri, M., Carraro, U., Podhorska-Okolov, M., Rizzi, C., Arslan, P., Monti, D., Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise (1995) FEBS Lett, 373 (3), pp. 291-295Van Royen, M., Carbo, N., Busquets, S., Alvarez, B., Quinn, L.S., Lopez-Soriano, F.J., DNA fragmentation occurs in skeletal muscle during tumor growth: A link with cancer cachexia? (2000) Biochem Biophys Res Commun, 270 (2), pp. 533-537Rosante, M.C., Robert-Pires, C.M., Marquetti, R.C., Baldissera, V., Perez, S.E.A., Ninomyia, M., Esteróides Anabólicos Androgênicos não promovem hipertrofia adicional em ratos submetidos a treinamento de força (2003) XVIII Reunião Anual Da FeSBE, p. 7. , CaxambuRobert-Pires, C.M., (2000) Estudo Dos Efeitos Da Administração de Esteróides Androgênicos Sobre O Diâmetro Das Fibras Musculares Esqueléticas de Ratos Jovens Submetidos Ou Não Ao Exercício Físico, , São Carlos, Dissertação de Mestrado - Centro de Ciências Biológicas e da Saúde da Universidade Federal de São CarlosDaugaard, J.R., Nielsen, J.N., Kristiansen, S., Andersen, J.L., Hargreaves, M., Richter, E.A., Fiber type-specific expression of GLUT4 in human skeletal muscle: Influence of exercise training (2000) Diabetes, 49 (7), pp. 1092-1095Chi, M.M., Hintz, C.S., Henriksson, J., Salmons, S., Hellendahl, R.P., Park, J.L., Chronic stimulation of mammalian muscle: Enzyme changes in individual fibers (1986) Am J Physiol, 251 (4 PART 1), pp. C633-42Santarém, J.M., Treinamento de força e potência (1999) O Exercício - Preparação Fisiológica, Avaliação Médica, Aspectos Especiais e Preventivos, pp. 35-50. , Ghorayeb N, Barros Neto TL. Rio de Janeiro: AtheneuFaulkner, J.A., Brooks, S.V., Opiteck, J.A., Injury to skeletal muscle fibers during contractions: Conditions of occurrence and prevention (1993) Phys Ther, 73, pp. 911-921Newham, D.J., Jones, D.A., Clarkson, P.M., Repeated high-force eccentric exercise: Effects on muscle pain and damage (1987) J Appl Physiol, 63, pp. 1381-1386Antonio, J., Wilson, J.D., George, F.W., Effects of castration and androgen treatment on androgen-receptor levels in rat skeletal muscles (1999) J Appl Physiol, 87 (6), pp. 2016-2019Krieg, M., Bartsch, W., Herzer, S., Becker, H., Voigt, K.D., Quantification of androgen binding, androgen tissue levels, and sex hormone-binding globulin in prostate, muscle and plasma of patients with benign prostatic hypertrophy (1977) Acta Endocrinol (Copenh), 86 (1), pp. 200-215Rand, M.N., Breedlove, S.M., Androgen locally regulates rat bulbocavernosus and levator ani size (1992) J Neurobiol, 23 (1), pp. 17-30Leslie, M., Forger, N.G., Breedlove, S.M., Sexual dimorphism and androgen effects on spinal motoneurons innervating the rat flexor digitorum brevis (1991) Brain Res, 561 (2), pp. 269-273Tingus, S.J., Carlsen, R.C., Effect of continuous infusion of an anabolic steroid on murine skeletal muscle (1993) Med Sci Sports Exerc, 25 (4), pp. 485-494Gibson, M.C., Schultz, E., Age-related differences in absolute numbers of skeletal muscle satellite cells (1983) Muscle Nerve, 6 (8), pp. 574-580Fidzianska, A., Kaminska, A., Apoptosis: A basic pathological reaction of injured neonatal muscle (1991) Pediatr Pathol, 11 (3), pp. 421-429Stangel, M., Zettl, U.K., Mix, E., Zielasek, J., Toyka, K.V., Hartung, H.P., H 2O 2 and nitric oxide-mediated oxidative stress induce apoptosis in rat skeletal muscle myoblasts (1996) J Neuropathol Exp Neurol, 55 (1), pp. 36-43Abu-Shakra, S.R., Alhalabi, M.S., Nachtman, F.C., Schemidt, R.A., Brusilow, W.S., Anabolic steroids induce injury and apoptosis of differentiated skeletal muscle (1997) J Neurosci Res, 47 (2), pp. 186-197Zaugg, M., Jamali, N.Z., Lucchinetti, E., Xu, W., Alam, M., Shafiq, A.S., Anabolic-androgenic steroids induce apoptotic cell death in adult rat ventricular myocytes (2001) J Cell Physiol, 187 (1), pp. 90-95Haupt, H.A., Rovere, G.D., Anabolic steroids: A review of the literature (1984) Am J Sports Med, 12 (6), pp. 469-48

The REAL (REctal Anastomotic Leak) score for prediction of anastomotic leak after rectal cancer surgery

Background Anastomotic leak after rectal cancer surgery is a severe complication associated with poorer oncologic outcome and quality of life. Preoperative assessment of the risk for anastomotic leak is a key component of surgical planning, including the opportunity to create a defunctioning stoma. Objective The purpose of this study was to identify and quantify the risk factors for anastomotic leak to minimize risk by either not restoring bowel continuity or protecting the anastomosis with a temporary diverting stoma. Methods Potentially relevant studies were identified from the following databases: PubMed, Embase and Cochrane Library. This meta-analysis included studies on transabdominal resection for rectal cancer that reported data about anastomotic leak. The risk for anastomotic leak after rectal cancer surgery was investigated. Preoperative, intraoperative, and postoperative factors were extracted and used to compare anastomotic leak rates. All variables demonstrating a p value 0.0791 to identify anastomotic leak were 79.1% and 32.9%, respectively. Accuracy of the threshold value was confirmed in the validation set with 77.8% sensitivity and 35.2% specificity. Conclusions We trust that, with further refinement using prospective data, this nomogram based on preoperative risk factors may assist surgeons in decision making. The score is now available online ().Surgical oncolog