56 research outputs found
Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic
therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods.
A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid
chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities
in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of
the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures
grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the
one with low cercosporin productioninfo:eu-repo/semantics/publishedVersio
Ankle brachial index (ABI) in a cohort of older women in the Philippines: Prevalence of peripheral artery disease and predictors of ABI
Objective: Cardiovascular disease (CVD) is rising in low and middle-income countries, but studies of CVD epidemiology in such settings often focus on risk factors rather than measures of disease progression. Here we use the ankle brachial index (ABI) to assess the prevalence of peripheral artery disease (PAD) among older women living in Metropolitan Cebu, Philippines, and relationships between ABI and CVD risk factors and body composition. Methods: ABI was measured using the Doppler technique in 538 female participants in the 2015 Cebu Longitudinal Health and Nutrition Survey (mean age 58 years, range 47-78 years). ABI was related to a panel of CVD risk factors measured in 2005 and 2012, and to 2012 body composition measures. Results: The prevalence of PAD (1.8%) was among the lowest reported in any comparably-aged sample, and only 9.9% of participants had an ABI indicating borderline PAD risk. Smoking (P < 0.011) and use of CVD medications (P < 0.0001) predicted lower ABI (indicating higher PAD risk), which was also lower in relation to 2012 systolic blood pressure (P < 0.054). ABI was unrelated to other CVD risk factors. An apparent protective relationship between body mass index (BMI) and ABI, noted in previous studies, was found to be confounded by protective relationships between ABI and fat free mass, height, and grip strength (all P < 0.05). Conclusions: The prevalence of PAD is low in Cebu Longitudinal Health and Nutrition Survey participants, and ABI was related to few CVD risk factors. Past reports of lower PAD risk in relation to BMI may reflect confounding by lean mass, which has protective relationships with ABI
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folĂculos prĂ©-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle nĂŁo-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial MĂnimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentraçÔes de 5, 10 ou 15M, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folĂculos prĂ©-antrais normais no controle nĂŁo-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle nĂŁo-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A anĂĄlise ultra-estrutural nĂŁo mostrou folĂculos prĂ©-antrais Ăntegros apĂłs cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α -tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentraçÔes testadas reduziu a viabilidade folicular apĂłs o cultivo in vitro. ______________________________________________________________________________________________________________ ABSTRACTThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15M, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture
A apropriação social da Internet pelo bibliotecårio catarinense: o retrato de uma década
Energetic status of crossbreed dairy cows during transition period in two different seasons
Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil
Home-range and space use by Didelphis albiventris (Lund 1840) (Marsupialia, Didelphidae) in Mutum Island, ParanĂĄ river, Brazil
Acidentes de trabalho afetando a coluna vertebral: um estudo realizado com trabalhadores de enfermagem de um hospital universitĂĄrio
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