44 research outputs found
A genome-wide identification and comparative analysis of the lentil MLO genes
Revista electrónica on linePowdery mildew is a widespread fungal plant disease that can cause significant losses in
many crops. Some MLO genes (Mildew resistance locus O) have proved to confer a durable
resistance to powdery mildew in several species. Resistance granted by the MLO gene family
members has prompted an increasing interest in characterizing these genes and implementing
their use in plant breeding. Lentil (Lens culinaris Medik.) is a widely grown food
legume almost exclusively consumed as dry seed with an average world production of 4.5
million tons. Powdery mildew causes severe losses on certain lentil cultivars under particular
environmental conditions. Data mining of the lentil CDC Redberry draft genome allowed to
identify up to 15 gene sequences with homology to known MLO genes, designated as
LcMLOs. Further characterization of these gene sequences and their deduced protein
sequences demonstrated conformity with key MLO protein characteristics such as the presence
of transmembrane and calmodulin binding domains, as well as that of other conserved
motifs. Phylogenetic and other comparative analyses revealed that LcMLO1 and LcMLO3
are the most likely gene orthologs related to powdery mildew response in other species,
sharing a high similarity with other known resistance genes of dicot species, such as pea
PsMLO1 and Medicago truncatula MtMLO1 and MtMLO3. Sets of primers were designed
as tools to PCR amplify the genomic sequences of LcMLO1 and LcMLO3, also to screen
lentil germplasm in search of resistance mutants. Primers were used to obtain the complete
sequences of these two genes in all of the six wild lentil relatives. Respective to each gene,
all Lens sequences shared a high similarity. Likewise, we used these primers to screen a
working collection of 58 cultivated and 23 wild lentil accessions in search of length polymorphisms
present in these two genes. All these data widen the insights on this gene family and
can be useful for breeding programs in lentil and close related species.S
Genome-wide Identification, Classification and Expression Analysis of the Mildew Resistance Locus O ( MLO ) Gene Family in Sweet Orange ( Citrus sinensis )
Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp.
IL PROBLEMA STORIOGRAFICO DELLA NOZIONE DI "FILOSOFIA SCOLASTICA" E LA GENESI DELLA NOZIONE DI "SECONDA SCOLASTICA"
Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling
Laser surface remelting and alloying of aluminium alloys
Aluminium alloys offer notable advantages in the manufacture of structural components, but generally they have poor tribological behaviour characteristics.Such disadvantages can be lessened by adequate surface treatments, which do not compromise the over-all properties of the material used Laser surface remelting and alloying treatments enable the obtainment of surface layers of an adequate thickness, characterized by distinct metallurgical properties.By laser surface alloying it is possible to alter the metallurgical and mechanical characteristics of the sublayer submitted to radiation by addition on its surface of a predetermined quantity of chemical elements melted together with the base material.By suitable techniques, it is possible to achieve sound alloyed layers of no less than one millimetre thick, with hardness values of over 200 HV, obtained with laser powers that are industrially acceptable in decidedly short treatment times.By simply remelting it is possible to drastically reduce or even eliminate pre-existing porosity in the material submitted to laser radiation by exploiting the rapid solidification of thin surface layers, and consequently remarkable benefits in terms of tensile strength and ductility are achieved and hence, also thermical fatigue resistance