An assessment of genetic fidelity of in vitro grown plantlets of rose (Rosa hybrida) through molecular markers

A simple and routine method for the analysis of somaclonal variation among tissue culture derived rose plants is a prerequisite for precise monitoring of quality control during rapid mass micropropagation. Random  amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) molecular marker techniques were employed to validate the genetic fidelity in three varieties of Rosa hybrida [Culture varieties (cvs) First  Red, Cri Cri and Pusa Gaurav] multiplied in vitro by axillary multiplication for up to 26 subcultures. Twelve  RAPD and seventeen ISSR primers generated a total of 119, 104 and 114 amplicons in cvs First Red, Cri Cri  and Pusa Gaurav, respectively. A homogeneous amplification profile was observed between the explant source and all the micropropagated plantlets. The result indicated the clonal fidelity of the tissue culture raised R.  hybrida plantlets and corroborated the assumption that axillary multiplication is the safest mode for  multiplication of true to type plants without any somaclonal variation.Key words: Rosa hybrida, in vitro, genetic fidelity, molecular markers

Study of Dissipative Collisions of 20^{20}Ne (∼\sim7-11 MeV/nucleon) + 27^{27}Al

The inclusive energy distributions of complex fragments (3 $\leq$Z $\leq$ 9) emitted in the reactions $^{20}$Ne (145, 158, 200, 218 MeV) + $^{27}$Al have been measured in the angular range 10$^{o}$ - 50$^{o}$. The fusion-fission and the deep-inelastic components of the fragment yield have been extracted using multiple Gaussian functions from the experimental fragment energy spectra. The elemental yields of the fusion-fission component have been found to be fairly well exlained in the framework of standard statistical model. It is found that there is strong competition between the fusion-fission and the deep-inelastic processes at these energies. The time scale of the deep-inelastic process was estimated to be typically in the range of $\sim$ 10$^{-21}$ - 10$^{-22}$ sec., and it was found to decrease with increasing fragment mass. The angular momentum dissipations in fully energy damped deep-inelastic process have been estimated from the average energies of the deep-inelastic components of the fragment energy spectra. It has been found that, the estimated angular momentum dissipations, for lighter fragments in particular, are more than those predicted by the empirical sticking limit.Comment: 16 pages, 9 figure

Evidence of large nuclear deformation of 32^{32}S∗^{*} formed in 20^{20}Ne + 12^{12}C reaction

Deformations of hot composite $^{32}$S$^{*}$ formed in the reaction $^{20}$Ne ($\sim$ 7 -- 10 MeV/nucleon) + $^{12}$C have been estimated from the respective inclusive $\alpha$-particle evaporation spectra. The estimated deformations for $^{32}$S$^{*}$ have been found to be much larger than the `normal' deformations of hot, rotating composites at similar excitations. This further confirms the formation of highly deformed long-lived configuration of $^{20}$Ne + $^{12}$C at high excitations ($\sim$ 70 -- 100 MeV) -- which was recently indicated from the analysis of the complex fragment emission data for the same system. Exclusive $\alpha$-particle evaporation spectra from the decay of hot composite $^{32}$S$^{*}$ also show similar behaviour.Comment: 9 pages, 6 figure

Prevalence of computer vision syndrome and associated ocular morbidity in a tertiary care hospital

Background: This study was done to determine the prevalence of computer vision syndrome and associated ocular morbidity in a tertiary care hospital. Methods: A cross sectional observational study was conducted on  673 patients based on the inclusion criteria. The patients were asked to fulfil the computer vision syndrome survey form 3 and OSDI questionnaire. Spontaneous eye blink rate, Schirmer’s test 1, tear break up time, refraction were performed for these patients. Data was collected and analysed by SPSS software. Results: The prevalence of computer vision syndrome was 73% with 294 males and 206 females. The mean age of the patients was 30.29 years. Most of the participants were between 21-30 years. The screen time for most of the study participants (59.80%) came out to be >6 hours/day on average. The chief complaint of majority patients was blurred vision and asthenopia whereas the secondary complaint was watering and headache. Maximum patients (38.20%) from our study were students and competitive exam aspirants followed by teachers and software engineers. The screen modality used by 34.20 % patients was mobile phones > laptops. The mean SEBR was 13.50 minutes. The mean OSDI was 17.44. The screen time was significantly correlated to SEBR (p<0.0001). Also the screen time and OSDI correlation came out to be significant (p<0.0001). Conclusions: The present study revealed a significantly high prevalence of CVS among our patients. The ocular symptoms are predominantly affecting the convergence power of eyes and tear film instability causing blurred vision and dryness

Characterization of fragment emission in ^{20}Ne (7 - 10 MeV/nucleon) + ^{12}C reactions

The inclusive energy distributions of the complex fragments (3 $\leq$ Z $\leq$ 7) emitted from the bombardment of ^{12}C by ^{20}Ne beams with incident energies between 145 and 200 MeV have been measured in the angular range 10$^{o} \leq \theta_{lab} \leq$ 50^{o}. Damped fragment yields in all the cases have been found to be the characteristic of emission from fully energy equilibrated composites. The binary fragment yields are compared with the standard statistical model predictions. Enhanced yields of entrance channel fragments (5 $\leq$ Z $\leq$ 7) indicate the survival of orbiting-like process in ^{20}Ne + ^{12}C system at these energies.Comment: 18 pages, 13 figure

How aware are we regarding vector borne diseases? A community based study in a slum of Kolkata, India

Background: Vector borne diseases (VBDs) form a major part of the communicable diseases in India. Ignorance and impoverished conditions of people contribute in creating source and spread of vector borne diseases and hinder disease control strategy. Slums are more vulnerable to vector borne diseases because of poor environmental condition, standard of living, poverty and ignorance of the people. This study is a small endeavour to highlight the awareness of residents of slum area of Chetla, Kolkata, West Bengal, India regarding vector borne diseases. Objectives were to assess the awareness of the study population regarding different vector borne diseases and to find out the association of awareness with relevant demographic variables.Methods: A community based observational, cross-sectional study was conducted among adult population in a slum area of Chetla, Kolkata, West Bengal, India. Multivariate logistic analysis was done to find out association of awareness with relevant variables.Results: Awareness regarding malaria was good and that of dengue was satisfactory while awareness regarding other vector borne diseases was poor. Age, sex, caste, education and social class were found significantly associated with satisfactory awareness. Younger population i.e. age ≤35years, males, general caste people, literacy status above primary school and social class III and above had better awareness regarding vector borne diseases.Conclusions: This study uncovered the lacunae regarding awareness of the study population regarding vector borne diseases. It can be concluded that intensified efforts towards creating public awareness and mobilizing the community regarding the identified issues should be addressed

The effects of changes in glutathione levels through exogenous agents on intracellular cysteine content and protein adduct formation in chronic alcohol-treated VL17A cells

Alcohol-mediated liver injury is associated with changes in the level of the major cellular antioxidant glutathione (GSH). It is interesting to investigate if the changes in intracellular GSH level through exogenous agents affect the intracellular cysteine content and the protein adduct formation indicative of oxidative insult in chronic alcohol treated liver cells. In VL-17A cells treated with 2mM N-acetyl cysteine (NAC) or 0.1mM ursodeoxycholic acid (UDCA) plus 100mM ethanol, an increase in cysteine concentration which was accompanied by decreases in hydroxynonenal (HNE) and glutathionylated protein adducts were observed. Pretreatment of 100mM ethanol treated VL-17A cells with 0.4mM buthionine sulfoximine (BSO) or 1mM diethyl maleate (DEM) had opposite effects. Thus, altered GSH level through exogenous agents may either potentiate or ameliorate chronic alcohol-mediated protein adduct formation and change the cysteine level in chronic alcohol treated VL-17A cells. The gene expression of non-treated and ethanol-treated hepatocytes in 2 microarray datasets was also compared to locate differentially expressed genes involved in cysteine metabolism. The study demonstrates that increased protein adducts formation and changes in cysteine concentration occur under chronic alcohol condition in liver cells which may increase alcohol-mediated oxidative injury

Scaling up, sustaining, and enhancing school-based sexuality education programs in resource-constrained and conservative contexts: Replicable lessons from positive-deviant countries

Despite considerable efforts, progress in the implementationof sexuality education (SE) has been uneven. This study identi-fied six “positive-deviant” low- and middle-income countries,i.e., countries that had scaled up, sustained and enhancedtheir SE programs when many others—in similar social, cul-tural and economic circumstances—were not able to do so. Inother words, they were significantly and consistently moresuccessful than the norm. Countries were shortlisted using avalidated framework and were analyzed using three other vali-dated frameworks on political priority setting, scaling up, andstakeholder engagement. The study found that India, Pakistan,Nigeria, Senegal, Mexico, and Uruguay had scaled up (eithernationwide or in some states/provinces), sustained andenhanced their SE programs in very different contexts. In allsix, SE was a political priority, the national or state/provincelevel SE scale up effort had been carefully planned and man-aged, and a mix of methods were used to build support and/or to overcome resistance. The study points to what needs tobe done better/more energetically/differently in research, pro-gram support-tool development, and policy and program sup-port to change the status quo

A Case Control Study on Risk Factors and Drug Prescription Patterns in Glaucoma at a tertiary eye care center in a city of Western India

Introduction: Glaucoma is an idiopathic, progressive optic disc neuropathy complicating into irreversible blindness if untreated. Early diagnosis by screening cases from high-risk populations has a pivotal role in managing this major public health problem with high treatment expenditures. Objectives: To identify the various ocular and non ocular risk factors of glaucoma and to identify the drug prescription pattern among glaucoma patients.Method: This was an observational, case-control study including 165 adult Glaucoma patients on treatment as cases and 165 age and sex-matched healthy individuals as controls, all of which were randomly selected from the patients visiting a tertiary eye care center. Various risk factors, drug prescription pattern and symptoms of the patients were recorded and analyzed.Results: A total of 165 adult Glaucoma patients and age and gender matched 165 controls were enrolled. Majority of the patients (41.21%) complained of blurring of vision at the time of study. The Odds ratios for Family history, Hypertension, Diabetes Mellitus, Migraine, Sleep apnea and Smoking showed strong association as risk factors for Glaucoma and the differences between the two groups were statistically significant (p value < 0.05). The mean number of drugs per prescription ± SD was 1.88 ± 0.79. Fixed drug formulations were prescribed in 42.4% patients. All the drugs were prescribed by their brand names and majority of them were in the form of eye drops.Conclusion: Primary Open Angle Glaucoma (POAG) was the most common subtype in the study. Age, Family history, Myopia, Hypertension, Diabetes Mellitus, Sleep Apnea, Migraine, Corticosteroid usage and Smoking emerged as putative risk factors. In consistence with present guidelines, Prostaglandin analogs were the most prescribed antiglaucoma drugs. The considerable proportion of asymptomatic cases (23%) suggests the need for periodic eye examinations to detect glaucomatous changes at an early stag