14 research outputs found
Occurrence of pancreatic ductal cell dysplasia in rats fed with a high fat diet and ethanol
The effects of alcohol and diet on acute
pancreatitis were studied in 192 male Wistar rats. The
animals were fed with standard laboratory food up to three
months of age and, after that, were divided into four groups
of 48 animals,each group receiving a different diet:
standard, fat-rich, protein-rich or carbohydrate-rich. In
each diet group, 24 animals obtained 15% (v/v) ethanol
in their drinking solution while the other 24 rats had water
ad libitum. The diet period lasted for 12 weeks, after which
acute experimental pancreatitis was induced under diethyl
ether anesthesia by ductal injection of rat bile into the
pancreatic ducts. Moderate or severe ductal cell dysplasia
developed in three of the 15 survivors in the group fed with
a high-fat diet and 15% ethanol in their drinking solution.
Mild acute pancreatitis was histologically found in 13 rats
and moderate pancreatitis in one rat in this group. One rat
did not show any pancreatic parenchyma1 changes. Two
of the rats with ductal cell dysplasia had mild pancreatitis
and the pancreas of the third rat was normal in this respect.
Dysplastic changes were not found in any other
experimental group used in the study. The observation is
statistically significant at p < 0.025 level. The results indicate
that alcohol and a high fat diet together might have a
carcinogenic effect on pancreatic ductal epithelium in rats