Utilization of crab shell-derived chitosan in nanoparticle synthesis for curcumin delivery

1183-1188Chitosan derived from crustaceans is biodegradable as well as biocompatible and can be made into nanoparticles when chelated with chelators, such as sodium tripolyphosphate and barium chloride. In this study, crab shells-derived chitosan was chelated using sodium trimetaphosphate to form nanoparticles. Curcumin was encapsulated into nanoparticles and characterized using Fourier transform infra-red spectroscopy, scanning electron microscopy, atomic force microscopy, and X-ray diffraction analysis. The particles were found to be 18 nm in size, while the curcumin-loaded particles were 25 nm in size. The particles were observed to encapsulate 90% of the drug used. The nanoparticles produced were analyzed for in vitro controlled drug release against Pseudomonas aeruginosa, Bacillus subtilis, and Candida albicans.</em

Chelators influenced synthesis of chitosan–carboxymethyl cellulose microparticles for controlled drug delivery

Abstract In this study, polyphenolic curcumin is entrapped within microcomposites made of biopolymers chitosan (CS) and carboxymethyl cellulose (CMC) formulated by ionic gelation method. Here, different concentrations of two chelating agents, barium chloride and sodium tripolyphosphate, are used to make microcomposites. Thus, the synthesized microparticles were characterized by FTIR, and their surface morphology was studied by SEM. Drug encapsulation efficiency and the drug release kinetics of CS–CMC composites are also studied. The produced microcomposites were used to study antibacterial activity in vitro

Purification, characterization and utilization of polysaccharide of Araucaria heterophylla gum for the synthesis of curcumin loaded nanocarrier

In this study, gum of Araucaria heterophylla was collected. The collected gum was subjected for extraction of polysaccharide using solvent extraction system. Thus, extracted polysaccharide was further purified using solvent method and was characterized using UV-Vis spectroscopy, Phenol sulfuric acid assay, FTIR, TGA, TLC and GC-MS. The gum derived polysaccharide was found to have the following sugars Rhamnose, Allose, Glucosinolate, Threose, Idosan, Galactose and Arabinose. The extracted polysaccharide was tested for various in-vitro bioactive studies such as antibacterial activity, antioxidant activity and anticancer activity. The polysaccharide was found to have antioxidant and anticancer activity. Further, the polysaccharide was subjected for carboxymethylation to favor the nanocarrier synthesis, where it was chelated using Sodium Tri Meta Phosphate (STMP) to form nanocarriers. The nanocarriers so formed were loaded with curcumin and were characterized using FTIR, SEM, EDX and AFM. Both the loaded and unloaded nanocarriers were studied for its in-vitro cytotoxic effect against the MCF7 human breast cancer cell lines. The nanocarriers were found to deliver the drug efficiently against the cancer cell line used in this study

Hypoxia in bone and oxygen releasing biomaterials in fracture treatments using mesenchymal stem cell therapy: a review

Bone fractures have a high degree of severity. This is usually a result of the physical trauma of diseases that affect bone tissues, such as osteoporosis. Due to its highly vascular nature, the bone is in a constant state of remodeling. Although those of younger ages possess bones with high regenerative potential, the impact of a disrupted vasculature can severely affect the recovery process and cause osteonecrosis. This is commonly seen in the neck of femur, scaphoid, and talus bone. In recent years, mesenchymal stem cell (MSC) therapy has been used to aid in the regeneration of afflicted bone. However, the cut-off in blood supply due to bone fractures can lead to hypoxia-induced changes in engrafted MSCs. Researchers have designed several oxygen-generating biomaterials and yielded varying degrees of success in enhancing tissue salvage and preserving cellular metabolism under ischemia. These can be utilized to further improve stem cell therapy for bone repair. In this review, we touch on the pathophysiology of these bone fractures and review the application of oxygen-generating biomaterials to further enhance MSC-mediated repair of fractures in the three aforementioned parts of the bone

Production, characterization and application of nanocarriers made of polysaccharides, proteins, bio-polyesters and other biopolymers: a review

Chitosan, collagen, gelatin, polylactic acid and polyhydroxyalkanoates are notable examples of biopolymers, which are essentially bio-derived polymers produced by living cells. With the right techniques, these biological macromolecules can be exploited for nanotechnological advents, including for the fabrication of nanocarriers. In the world of nanotechnology, it is highly essential (and optimal) for nanocarriers to be biocompatible, biodegradable and non-toxic for safe in vivo applications, including for drug delivery, cancer immunotherapy, tissue engineering, gene delivery, photodynamic therapy and many more. The recent advancements in understanding nanotechnology and the physicochemical properties of biopolymers allows us to modify biological macromolecules and use them in a multitude of fields, most notably for clinical and therapeutic applications. By utilizing chitosan, collagen, gelatin, polylactic acid, polyhydroxyalkanoates and various other biopolymers as synthesis ingredients, the ‘optimal’ properties of a nanocarrier can easily be attained. With emphasis on the aforementioned biological macromolecules, this review presents the various biopolymers utilized for nanocarrier synthesis along with their specific synthetization methods. We further discussed on the characterization techniques and related applications for the synthesized nanocarriers

Lignocellulose Derived Nanocellulose, Its Properties, and Applications - A Review

Natural fibers are abundant in natural resources. Natural fibers have been used in various applications. Natural fibers have been proved to be extremely useful in multiple fields in the world.&nbsp; Natural fibers possess great mechanical and optical properties. Nanocellulose fibers are obtained from plants and it has many applications. It can be used as a nanocomposite. They can be extracted by performing various techniques. It is used as a barrier due to its crystalline structure, which makes it difficult for molecules to flow through. Nanocellulose fibers are biodegradable, strong, lightweight, low density, and renewable since they have been produced from natural resources. They have immense applications in electricals, nanotechnology, medicine, drug delivery, aerospace, adsorbents, papermaking, and dental.&nbsp; The following review will focus on the properties of lignocellulose-derived nanocellulose, cellulose nanocrystals (CNCs), and cellulose nanofibrils (CNFs) and their applications in nanotechnology

Study of Various Properties of Chemically Treated Lignocellulosic Cissus quadrangularis Stem Fiber for Composite Reinforcement

The increasing demand for natural fiber-reinforced composites has opened up the market for inexpensive, lightweight, bio-renewable, and environment-friendly plant fibers. The chemical treatments on fiber lead to the reduction of lignin and hemicellulose contents which helps in better adhesion with the matrix. The objective of this work is to do various chemical treatments on Cissus quadrangularis Stem Fiber (CQSF) and perform its characterization. The natural fibers are first extracted from the Cissus quadrangularis stem using the retting process. The fibers are then chemically treated with magnesium carbonate (MgCO3), sodium hydroxide (NaOH), sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3), and calcium hydroxide (Ca(OH)2). The characterization of single fibers is investigated by single-fiber tensile test, chemical composition, thermogravimetric analysis, and field emission scanning electron microscope. Characterization results show that the MgCO3-treated CQSF has improved mechanical and thermal properties. Thus, MgCO3-treated CQSF is suggested for biocomposite preparation due to its promising mechanical properties and thermal properties

Utilization of chitosan-coated superparamagnetic iron oxide nanoparticles for chromium removal

Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used for their versatility, while it is coated with a biopolymer like chitosan that adds attraction and also increases its applications. In this study, SPION was synthesized by chemical co-precipitation method, characterized using various analytical techniques like UV–Vis, FTIR, SEM, EDX, TEM, AFM, XRD, zeta potential and Raman spectroscopy analysis. Chitosan was coated onto the SPIONs and used for water treatment to remove chromium (450 ppm concentration). Chitosan-coated SPIONs were found to remove about 80% of chromium. Freundlich model was found to be fitting better for the current study

CHARACTERIZATION OF POLYHYDROXYBUTYRATE SYNTHESIZED BY BACILLUS CEREUS

Objectives: To characterize the Polyhydroxybutyrate (PHB) produced from Bacillus cereus using various instrumental methods and reduce them to the nanoscale which can be used as drug carrierMethods: The isolated bacterium was identified by 16S rDNA analysis. PHB produced by the bacterium was extracted by a process using boiling chloroform followed by methanol which selectively precipitates PHA. The polymer produced was analyzed using various instrumental techniques like FTIR, GC-MS, 1Hand 13C NMR, XRD and FESEM. This biogenic PHB has been reduced to nanospheres which were analyzed by FESEM.Results: A bacterium with the ability to produced PHB was isolated from the cloth used to smear oil on pan cake pan which was identified as Bacillus cereus by 16S rDNA analysis. The organism was capable of accumulating 1.19 g L-1 of PHA corresponding to 49.7% of its dry weight after 48 h of incubation. The polymer produced was analyzed using various instrumental techniques which identified the polymer as PHB.Conclusion: The organism seems to be a potential candidate for the biogenic synthesis of PHB which can find application as drug carrier.Â