Fluorescence in situ hybridisation study of micronuclei in C3A cells following exposure to ELF-magnetic fields

Human C3A cells were exposed to extremely low frequency (50 Hz) magnetic fields (ELF-MF's) up to 500 mu T. They were subjected to the micronucleus assay using a Fluorescence In Situ Hybridization (FISH) technique with an in-house pan-centromere probe. We found no increased frequency in micronucleated cells and no change in the proportion of centromere positive over centromere negative micronuclei compared to the unexposed control cells. These results are in accordance with some, but in contradiction with other previously published investigations underlining that effects of environmental ELF-EMF's on cellular DNA may be very subtle and that small changes or environmental influences may determine the outcome of a (geno)toxicity study. Interestingly, a low-level (5 mu T) exposure resulted in less than the background micronucleus frequency

Mycobacterieel conjunctivaal granuloom bij een Chinese zaagbekeend (Mergus squamatus)

A five-year-old, female scaly-sided merganser was presented with a chronic recurrent conjunctivitis of the left eye. Physical examination showed a caseous nodular lesion at the palpebral conjunctiva associated with excessive tearing. Necropsy revealed caseous necrotic debris at the left conjunctiva and infra-orbital sinus, but also multiple granuloma in several internal organs. Histological examination showed granulomatous splenitis, hepatitis, arteritis, fibrinonecrotic enteritis and conjunctivitis, and also amyloidosis of liver and spleen. Bacterial and molecular tests of a conjunctival swab in the live animal showed the presence of a multiresistant Escherichia coli strain and Chlamydia psittaci, respectively. These bacteria had only a secondary pathogenic role. No premortem cytological examination or Ziehl-Neelsen (ZN) staining of the histological section of the conjunctiva biopt or additional tests, such as blood test and radiographs, were performed. Finally, a Mycobacterium avium infection of the conjunctiva and internal organs was confirmed following necropsy by ZN staining and PCR analysis. Local and systemic antimicrobial treatments with fluoroquinolones, tetracyclines and aminoglycosides were unsuccessful

The impact of deoxynivalenol on pigeon health : occurrence in feed, toxicokinetics and interaction with salmonellosis

Seed-based pigeon diets could be expected to result in exposure of pigeons to mycotoxins such as deoxynivalenol (DON). Ingestion of low to moderate contamination levels of DON may impair intestinal health, immune function and/or pathogen fitness, resulting in altered host-pathogen interactions and thus different outcome of infections. Here we demonstrate that DON was one of the most frequently detected mycotoxins in seed-based racing pigeons feed, contaminating 5 out of 10 samples (range 177-1,466 mu g/kg). Subsequently, a toxicokinetic analysis revealed a low absolute oral bioavailability (F) of DON in pigeons (30.4%), which is comparable to other avian species. Furthermore, semi-quantitative analysis using high-resolution mass spectrometry revealed that DON-3 alpha-sulphate is the major metabolite of DON in pigeons after intravenous as well as oral administration. Following ingestion of DON contaminated feed, the intestinal epithelial cells are exposed to significant DON concentrations which eventually may affect intestinal translocation and colonization of bacteria. Feeding pigeons a DON contaminated diet resulted in an increased percentage of pigeons shedding Salmonella compared to birds fed control diet, 87 +/- 17% versus 74 +/- 13%, respectively. However, no impact of DON was observed on the Salmonella induced disease signs, organ lesions, faecal and organ Salmonella counts. The presented risk assessment indicates that pigeons are frequently exposed to mycotoxins such as DON, which can affect the outcome of a Salmonella infection. The increasing number of pigeons shedding Salmonella suggests that DON can promote the spread of the bacterium within pigeon populations

Development of an in vivo lipopolysaccharide inflammation model to study the pharmacodynamics of COX-2 inhibitors celecoxib, mavacoxib, and meloxicam in cockatiels (Nymphicus hollandicus)

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used frequently in avian medicine for their antipyretic, analgesic, and anti-inflammatory properties during surgery and for diseases that cause tissue damage and inflammation. NSAIDs inhibit cyclooxygenase (COX) enzymes, which are responsible for the induction of pyresis, pain, and inflammation. In our study, a lipopolysaccharide-induced (LPS) pyresis model was optimized using cockatiels (Nymphicus hollandicus) as subject birds (four males/three females) and validated in two females and one male, characterized by an intravenous bolus injection of LPS (7.5 mg/kg) administered at T-0 and T-24 (24 hours following the first LPS injection). To demonstrate the feasibility of the model to assess pharmacodynamic (PD) parameters of different NSAIDs, mavacoxib 4 mg/kg (four males/four females), celecoxib 10 mg/kg (four males/four females) and meloxicam 1 mg/kg (four males/four females) were evaluated in the model at dosages used frequently in practice. The PD parameters (body temperature, mentation, posture, preference of location in the cage, and prostaglandin E-2 [PGE(2)] plasma concentrations) were determined for 10 hours following the second LPS injection. At the doses evaluated, mavacoxib and celecoxib significantly reduced LPS-induced hypothermia, but had no clear effects on other clinical signs of illness. In contrast, no effect on hypothermia or clinical appearance was observed in the LPS-challenged cockatiels treated with meloxicam. All three NSAIDs were able to inhibit the increase in LPS-induced PGE2 plasma concentrations, yet the effect was most pronounced in the birds treated with meloxicam. Consequently, the presented model opens perspectives for future dose-effect PD studies to optimize analgesic protocols in cockatiels