Royal Jelly: An ancient remedy with remarkable antibacterial properties

Royal Jelly (RJ), a honeybee hypopharyngeal gland secretion of young nurse and an exclusive nourishment for bee queen, has been used since ancient times for care and human health and it is still very important in traditional and folkloristic medicine, especially in Asia within the apitherapy. Recently, RJ and its protein and lipid components have been subjected to several investigations on their antimicrobial activity due to extensive traditional uses and for a future application in medicine. Antimicrobial activities of crude Royal Jelly, Royalisin, 10-hydroxy-2-decenoic acid, Jelleines, Major Royal Jelly Proteins against different bacteria have been reported. All these beehive products showed antimicrobial activities that lead their potential employment in several fields as natural additives. RJ and its derived compounds show a highest activity especially against Gram positive bacteria. The purpose of this Review is to summarize the results of antimicrobial studies of Royal Jelly following the timescale of the researches. From the first scientific applications to the isolation of the single components in order to better understand its application in the past years and propose an employment in future studies as a natural antimicrobial agent

Beeswax: A minireview of its antimicrobial activity and its application in medicine

Abstract Beeswax is the substance that forms the structure of a honeycomb; the bees secrete wax to build the honeycombs where to store honey. Thanks to its rich hydrophobic protective properties, the beeswax is in fact present within cosmetics and body products. Also, beeswax is used in the food industry: as a film to wrap cheese for maturing or as a food additive (E901) to give shine to the products. Exactly as the honey which it contains, beeswax is also characterized by several therapeutic properties of great interest to us; it is thought to be particularly effective in healing bruises, inflammation and burns. Recently, the interest of researchers has moved even on antimicrobial properties of beeswax although there are still few studies in the literature focused only on the action of beeswax. The few studies showed an antimicrobic effectiveness of beeswax against overall Staphylococcus aureus , Salmonella enterica , Candida albicans and Aspergillus niger ; these inhibitory effects are enhanced synergistically with other natural products such as honey or olive oil. This minireview aims to be a collection of major scientific works that have considered the antimicrobial activity of beeswax alone or in combination with other natural products in recent years

Scientific note: varroa mite eradication, the strange case of Gorgona Island

Varroa mites are vectors for several bee viruses contributing also to their diffusion worldwide (Martin 2001; Di Prisco et al. 2011; Cersini et al. 2013). The tripartite relationship among bees, mites, and viruses is thought to be responsible for the loss of a large amount of colonies (Highfield et al. 2009; Berthoud et al. 2010; Francis et al. 2013). Before the arrival of Varroa destructor , virus prevalence was lower (Martin et al. 2012). The mites have contributed to spread the viruses which could be correlated to the high number of mite-infested honey bee colony losses (Berthoud et al. 2010; Francis et al. 2013). Since the varroa mite arrival, control of varroosis has become necessary (Moritz et al. 2010). Such control is focused on the coexistence between honeybees and mites. A trial to eradicate the mite from isolated areas has not been successful. Experiments carried out to eradicate varroa mite by use of several coordinated treatments in Jersey, an island in the English Channel, did not succeed, showing how difficult it is to eradicate this ecto-parasite from an infested area (Sampson and Martin 1999). The aim of this study is to report the disappearance of varroa mite from the island of Gorgona (43° 26′ N; 9° 54′ E) following several treatments in a single apiary kept in complete isolation. Furthermore, after the varroa disappearance, the honeybee viral load has been analyzed. Gorgona Island has been chosen because it provides suitable conditions to guarantee continuous monitoring and isolation to avoid new reintroduction of bee pathogens and pests

Phenoloxidase activity and haemolymph cytology in honeybees challenged with a virus suspension (deformed wings virus DWV) or phosphate buffered suspension (PBS)

The innate immune system of honeybees mainly consists in antimicrobial peptides, cellular immunity and melanisation. In order to investigate the immune response of honeybees to immune stressors, three stress degrees were tested. Newly emerged bees naturally DWV-infected were collected from a Varroa mite free apiary and divided into three experimental groups: naturally DWV infected bees. PBS injected bees. and artificially DWV super infected bees. Phenoloxidase activity and haemolymph cellular subtype count were investigated. Phenoloxidase activity was highest (P<0.05) in DWV-superinfected bees. and the haemocyte population differed within the three observed groups. Although. immune responses following DWV infection have still not been completely clarified. this investigation sheds light on the relation between cell immunity and the phenoloxidase activity of DWV naturally infected honeybees exposed to additional stress such as injury and viral superinfection

Next generation sequencing study on RNA viruses of Vespa velutina and Apis mellifera sharing the same foraging area

The predator Asian hornet (Vespa velutina) represents one of the major threats to honeybee survival. Viral spillover from bee to wasp has been supposed in several studies, and this work aims to identify and study the virome of both insect species living simultaneously in the same foraging area. Transcriptomic analysis was performed on V. velutina and Apis mellifera samples, and replicative form of detected viruses was carried out by strand‐specific RT‐PCR. Overall, 6 and 9 different viral types were reported in V. velutina and A. mellifera, respectively, and five of these viruses were recorded in both hosts. Varroa destructor virus‐1 and Cripavirus NB‐1/2011/HUN (now classified as Triato‐like virus) were the most represented viruses detected in both hosts, also in replicative form. In this investigation, Triato‐like virus, as well as Aphis gossypii virus and Nora virus, was detected for the first time in honeybees. Concerning V. velutina, we report for the first time the recently detected honeybee La Jolla virus. A general high homology rate between genomes of shared viruses between V. velutina and A. mellifera suggests the efficient transmission of the virus from bee to wasp. In conclusion, our findings highlight the presence of several known and newly reported RNA viruses infecting A. mellifera and V. velutina. This confirms the environment role as an important source of infection and indicates the possibility of spillover from prey to predator

Gray Wolf (Canis lupus italicus) and Red Fox (Vulpes vulpes) Parasite Survey in Anthropized and Natural Areas of Central Italy

Simple Summary Studies on wild animal parasites are considered crucial for the adoption of effective strategies aimed at reducing the impact of these pathogens on evolving ecosystems. This study aimed to assess and compare gastrointestinal nematodes and protozoa and other parasites detectable with coprological analysis in free-ranging wolf and red fox populations living in natural and anthropized areas of Tuscany (Central Italy). This comparison allowed us to detect significant differences in the occurrence and frequency of some parasite taxa considering the same canid species in different environments (natural and anthropized) and the two canid species in the same environment. Data obtained in this study may indicate different parasite risks and different roles played by the wolf and the fox in the diffusion of specific parasite taxa in the environments considered herein. Gastrointestinal nematodes and protozoa and other parasite occurrences were evaluated in free-ranging wolf (Canis lupus italicus) and red fox (Vulpes vulpes) populations from natural and anthropized areas of Central Italy. Analyzed fecal samples were collected from 60 foxes and 40 wolves in the anthropized areas, and 41 foxes and 39 wolves in the natural areas. In foxes, hookworm infections (p < 0.0001) were more frequently recorded in the anthropized environment, while coccidia (p < 0.05) and Cryptosporidium spp. (p < 0.0001) were more frequent in the natural area. In wolves, a higher frequency of hookworms (p < 0.0001) was observed in natural areas, while coccidia were more common in the anthropized area (p < 0.05). Moreover, in the natural environment, trichuroid nematodes (p < 0.0001) were significantly more frequent in wolves than in foxes, while Cryptosporidium (p < 0.001) and Giardia duodenalis (p < 0.001) were more common in foxes. In the anthropic area, the occurrence of hookworms was found to be significantly higher in foxes (p < 0.0001), while trichuroid nematodes were more common in wolves (p < 0.0001). The obtained data are indicative of a different diffusion of specific parasite taxa in wolves and foxes living in the natural and/or anthropized environments examined herein

Dietary supplementation of quebracho and chestnut tannins mix in rabbit: effects on live performances, digestibility, carcase traits, antioxidant status, faecal microbial load and economic value

The effects of dietary supplementation of tannins mix (quebracho and chestnut) in rabbit diet were evaluated for productive performances, health parameters and digestibility in order to quantify their practical utilisation in the rearing system. One-hundred and twenty Martini group hybrid rabbits of 30 days old were fed four different diets for 60 days. The diets were formulated as: basal diet (negative control, C), basal diet supplemented by 0.3% of tannins mix (T0.3), basal diet supplemented by 0.6% of tannins mix (T0.6) and basal diet supplemented with coccidiostat (positive control, CC). Live performances did not show any significant differences, moreover, no significant differences were observed for all carcase traits except for gastrointestinal tract (p =.015, lowest values for T0.3 diet). Also, digestibility of the feed and faecal microbial load was not influenced by tannins addition. Slight differences between the diets were detected in catalase and glutathione peroxidase concentrations in plasma, as a common trend was revealed with higher values of C than the other diets. From an economical point of view, T0.3 diet showed to be more profitable than CC and T0.6. Tannins addition might be taken into account as potential feed additive in rabbit feeds, as it does not affect negatively the productive performances, digestibility and induce a slight increase of antioxidant status.Highlights On an economical point of view tannin diet showed to be more profitable than a diet added with coccidiostat. Addition of tannins in rabbits’ feed did not affect negatively productive performances and digestibility. Tannins represent a valuable feed additive in rabbit farming as a slightly increase of antioxidant status was induced

Effects of nonprotein amino acids on survival and locomotion of Osmia bicornis

To investigate the effects of two non-protein amino acids (NPAAs), β-alanine and γ-amino butyric acid (GABA), on Osmia bicornis survival and locomotion, two groups of ten caged bees have been fed with a sugar syrup enriched with β-alanine and GABA, respectively. The further control group was fed with the sugar syrup. Five behavioural categories have been chosen in accordance with the principle of parsimony and with their intrinsic unitary consistency from onset to the end. Two states (staying under the paper and staying in the test tubes) and three events (walking on the net, feeding on the flower and flying) have been recorded by scan sampling. Furthermore, we analysed the amino acids content of the haemolymph that was sampled from additional forty-five bees kept under the same diet condition (15 per diet type). Bees fed with ß-alanine had a significant shorter survival than those fed with control and GABA diets. The GABA diet induced a higher locomotion than the β-alanine. The former non-protein amino acid was detected in the haemolymph of only bees fed with GABA. Results suggest that insects consuming NPAAs-reach diets can absorb and transfer these substances into the haemolymph and those NPAAs affect both survival and locomotion. Ecological consequences are discussed in the frame of plant reproductive biology