Galaxy evolution in nearby galaxy groups. III. A GALEX view of NGC 5846, the largest group in the local universe

We explore the co-evolution of galaxies in nearby groups (V < 3000 km/s) with a multi-wavelength approach. We analyze GALEX far-UV (FUV) and near-UV (NUV) imaging and SDSS u,g,r,i,z data of groups spanning a large range of dynamical phases. We characterize the photometric properties of spectroscopically-confirmed galaxy members and investigate the global properties of the groups through a dynamical analysis. Here we focus on NGC 5846, the third most massive association of Early-Type Galaxies (ETG) after the Virgo and Fornax clusters. The group, composed of 90 members, is dominated by ETGs (about 80 per cent), and among ETGs about 40\% are dwarfs. Results are compared with those obtained for three groups in the LeoII cloud, which are radically different both in member-galaxy population and dynamical properties. The FUV-NUV cumulative colour distribution and the normalized UV luminosity function (LF) significantly differ due to the different fraction of late-type galaxy population. The UV LF of NGC 5846 resembles that of the Virgo cluster, however our analysis suggests that star-formation episodes are still occurring in most of the group galaxies, including ETGs. The NUV-i colour distribution, the optical-UV colour-colour diagram, and NUV-r vs. Mr colour-magnitude relation suggest that the gas contribution cannot be neglected in the evolution of ETG-type group members. Our analysis highlights that NGC~5846 is still in an active phase of its evolution, notwithstanding the dominance of dwarf and bright ETGs and its virialized configuration.Comment: 26 pages, 13 figures, accepted for publication in MNRA

Galaxy evolution in groups. NGC 3447/NGC 3447A: the odd couple in LGG 225

Local Group Analogs (LGA) are galaxy associations dominated by few bright Spirals, reminiscent of the LG. The NGC3447/NGC3447A system, member of the LGG 225 group, a nearby LGA, is considered a physical pair: an intermediate luminosity late type spiral, NGC3447, and an irregular companion, NGC3447A, linked by a faint filament of matter. A ring-like structure in the NGC3447 outskirts is emphasised by UV observations. This work aims to contribute to the understanding of galaxy evolution in low density environments, favourable habitat to highly effective encounters. We performed a multi-wavelength analysis of the surface photometry of this system to derive spectral energy distribution and structural properties using UV and optical images. We also characterised the velocity field of the pair using new kinematic observations. All these data are used to constrain smooth particle hydrodynamic simulations with chemo-photometric implementation to shed light on the evolution of this system. Luminosity profiles are all consistent with the presence of a disc extending and including NGC3447A. The overall velocity field does not emphasise any significant rotation pattern, rather a small velocity gradient between NGC3447 and NGC3447A. Our simulation, detached from a large grid explored to best-fit the global properties of the system, suggests that this arises from an encounter between two halos of equal mass. NGC3447 and NGC3447A belong to the same halo, NGC3447A being a substructure of the same disk as NGC3447. The halo gravitational instability, enhanced by the encounter, fuels a long lived instability in this dark matter dominated disk, driving its morphology. This system may warn about a new class of "false pairs" and the potential danger of a misunderstanding of such objects in pair surveys that could produce a severe underestimate of the total mass of the system. (abridged)Comment: 14 pages, 10 figures, A&A accepte

Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis

The hypothalamus–pituitary–testis axis controls the production of spermatozoa, and the kisspeptin system, comprising Kiss1 and Kiss1 receptor (Kiss1R), is the main central gatekeeper. The activity of the kisspeptin system also occurs in testis and spermatozoa, but currently the need of peripheral kisspeptin to produce gametes is not fully understood. Hence, we characterized kisspeptin system in rat spermatozoa and epididymis caput and cauda and analyzed the possible presence of Kiss1 in the epididymal fluid. The presence of Kiss1 and Kiss1R in spermatozoa collected from epididymis caput and cauda was evaluated by Western blot; significant high Kiss1 levels in the caput (p < 0.001 vs. cauda) and constant levels of Kiss1R proteins were observed. Immunofluorescence analysis revealed that the localization of Kiss1R in sperm head shifts from the posterior region in the epididymis caput to perforatorium in the epididymis cauda. In spermatozoa-free epididymis, Western blot revealed higher expression of Kiss1 and Kiss1R in caput (p < 0.05 vs. cauda). Moreover, immunohistochemistry revealed that Kiss1 and Kiss1R proteins were mainly localized in the secretory epithelial cell types and in contractile myoid cells, respectively. Finally, both dot blot and Elisa revealed the presence of Kiss1 in the epididymal fluid collected from epididymis cauda and caput, indicating that rat epididymis and spermatozoa possess a complete kisspeptin system. In conclusion, we reported for the first time in rodents Kiss1R trafficking in spermatozoa during the epididymis transit and Kiss1 measure in the epididymal fluid, thus suggesting a possible role for the system in spermatozoa maturation and storage within the epididymis

Investigating early-type galaxy evolution with a multi-wavelength approach. III. Insights from SPH simulations with chemo-photometric implementation

We are exploring galaxy evolution in low density environments exploiting smooth particle hydrodynamic simulations including chemo-photometric implementation. From a large grid of simulations of galaxy encounters and mergers starting from triaxial halos of gas e dark matter, we single out the simulations matching the global properties of our targets. These simulations are used to give insights into their evolution. We focus on 11 early-type galaxies selected because of their nearly passive stage of evolution in the nuclear region. However, a variety of UV features are detected in more than half of these galaxies. We find no significant differences in the formation mechanisms between galaxies with or without UV features. Major and minor mergers are able to reproduce their peculiar UV morphologies, galaxy encounters are more suitable for 'normal' early-type galaxies. Their star formation rate self-quenches several Gyr later the merger/encounter occurred, via gas exhaustion and stellar feedback, moving the galaxy from blue to red colors, driving the galaxy transformation. The length of the quenching is mass dependent and lasts from 1 to 5 Gyr or more in the less massive systems. All our targets are gas rich at redshift 1. Three of them assembled at most 40% of their current stellar mass at z>1, and seven assembled more than 50% between redshift 0.5 and 1. Their stellar mass grows with 4% by crossing the Green Valley before reaching their current position on the NUV-r vs. Mr diagram.Comment: 23 pages, 8 figures, accepted in Ap

Case Report: First Report of Fatal Legionella pneumophila and Klebsiella pneumoniae Coinfection in a Kidney Transplant Recipient

A very rare case of pulmonary Klebsiella pneumoniae-Legionella pneumophila coinfection in a double kidney transplanted man affected by the chronic renal disease is described. Cases of Legionnaires’ disease with an incubation period of 14 days have rarely been documented. Despite the long period of hospitalization, typing of clinical and environmental L. pneumophila strains demonstrated that the patient’s home water distribution system was the source of infection, highlighting that Legionella house contamination can be a hidden risk, especially for immune-compromised people

Anti-obesity drug therapy in clinical practice: Evidence of a poor prescriptive attitude

Obesity is a worldwide growing problem for the health care systems and its treatment is strongly recommended. Orlistat, naltrexone/bupropion, and liraglutide are approved for weight loss in Italy in patients with a Body Mass Index (BMI) ≥ 30 kg/m2 or ≥ 27 kg/m2 with concomitant diseases. However, the prescription of these drugs is significantly low worldwide. General practitioners (GPs) play a key role in the early diagnosis and appropriate management of obesity. The aim of the study was to investigate the management of obesity and the prescriptive attitude of anti-obesity drugs in a general practice setting.All patients registered in lists of 8 GPs with a recorded diagnosis of obesity or BMI values ≥ 30 kg/m2 in the period 2017–2018, were recruited. A descriptive analysis of demographic and clinical characteristic was carried out. The Spearman's correlation rank test was applied to identify correlations between BMI and all the variables of interest.Among 1301 obese patients, only 66.1 % had been diagnosed and 29.4 % had no registered BMI value. Patients with recorded BMI, were overweight (7.8 %) or in the obesity class I (38.8 %), class II (14.1 %), and class III (7.1 %), respectively.The obese patients (class 1–3) were older [66 (55–76) vs 49 (32–59); p < 0.01], and had more concurrent diseases [5 (3−8) vs 4 (2–6); p < 0.01] than patients who reached a BMI < 30 Kg/m2. Moreover, most of obese were high cardiovascular risk (HCVr) patients (67.0 % vs 31.9 %; p < 0.01). The BMI was directly related to age (rs 0.14; p < 0.01), diabetes (rs 0.19; p < 0.01), hypertension (rs 0.14; p < 0.01), heart failure (rs 0.09; p < 0.01), HCVr (rs 0. 12; p < 0.01) and number of comorbidities (rs 0.08; p = 0.01). No prescriptions of orlistat or naltrexone/bupropion were found. Liraglutide was prescribed only in 7 patients because of the concomitant presence of diabetes.Our results suggest a low adherence to guide line recommendations for obesity management and confirm an under-prescription of anti-obesity drugs in Italy

Vertical Second Harmonic Generation in Asymmetric Dielectric Nanoantennas

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Intensive training programme for ultrasound-guided minimally invasive synovial tissue biopsy on knees and wrists in different phases of inflammation

Objectives: To develop an intensive training programme for ultrasound (US)-guided synovial tissue (ST) biopsy on knees and wrists in inflammatory arthritis and to assess the learning curve, patient tolerability, sample quality and trainees’ expectations. Methods: Active or remission rheumatoid arthritis patients were enrolled. Nine trainees joined the 4-month programme in a centre experienced in performing US-guided ST biopsies consisting of four sequential phases: (1) observation, (2) performance of guided step-by-step phases, (3) execution of the whole procedure on paired joints (knees or wrists) of the same patient in parallel with the trainer and (4) performance of the procedure autonomously. Sample representativity was assessed by histology, and procedure-related adverse events were recorded. Before and after the programme, trainees’ expectations and perceptions were collected. Results: 328 ST biopsy procedures were included. The rate of trainees’ informative samples was: (1) comparable to the trainers in active and remission knees, but lower in active wrists (70% for trainees vs 100% for trainers, p=0.06) in phase 3; (2) excellent on active knees and wrists (91.9% and 90.9% respectively) but lower (77.6%, p=0.0089) on remission knees in phase 4. Procedures performed by trainees did not affect patient tolerability. Trainees’ expectations about procedure-related invasiveness and pain infliction decreased while the difficulty of procedure execution on active wrists and remission knees remained perceived as moderately difficult. Conclusions: This intensive training programme develops advanced skills in the performance of US-guided ST biopsy on knees and wrists, yielding high-quality specimens available for basic and translational studies on inflammatory joint diseases

Beta-amyloid-acetylcholine structural interaction: evidence for neuroprotective effects of acetylcholine in neural cells

Alzheimer’s disease (AD) is regarded as a multifactorial disease characterized by a complex pathogenesis including a cholinergic deficit - due to degeneration of cholinergic projections from the basal forebrain - and the extracellular accumulation of amyloid beta (Aβ) peptide. Aβ containing 39 to 42 amino acids is the predominant component of the senile plaques that, together with neurofibrillary tangles, are regarded as the neuropathological hallmarks of AD (Sorrentino et al. 2014). Aβ may assume different conformations changing from random coil or α-helical monomers to β-sheet structures forming toxic oligomers and/or β-sheet mature fibrils. In this framework, we studied the effect of acetylcholine (ACh) on the conformation of Aβ by circular dichroism analysis. Moreover we investigated the ability of ACh to protect neuronal cells from the toxic action of amyloid peptide and to modulate the neuroinflammatory response occurring via the phospholipase A2 (PLA2). Results show that the amount of Aβ(25-35) β-strand raised linearly in absence of ACh, whereas it remained almost constant in presence of ACh. In addition, in a micelle solution mimicking the membrane environment ACh was found effective in increasing and stabilizing the soluble and not toxic helical content of Aβ(25-35) suggesting that ACh is capable to preserve the soluble form of Aβ(25-35), reducing the incipit of Aβ aggregation. In order to assess the neuro-protective ability of ACh against toxic Aβ(25-35) accumulation, we used neural cell (NCC) cultures containing both astrocytes and glial cells prepared from brains embryos from timed pregnant Wistar rats and infused ACh for 48h. By immunostaining, we observed that ACh reduced Aβ(25-35)-induced cell death. Then, we tested the protective effect of ACh on inflammation induced by Aβ administration. NCC were challenged with Aβ(25-35) in the presence and absence of ACh and immunostained for astroglial and neuronal markers: results showed a reduction of the morphological features of astrogliosys in ACh treated cells. PLA2 expression analysis corroborated these data also underlying that ACh can negatively regulate inflammation pathways in glial cells