Inside the secret garden of political parties. Transformations and reactions to primary elections in Italy and France

Primary elections are becoming quite spread within no-US political context. Many political parties in different countries choose to adopt such inclusive methods to select their candidates for general elections. Thus, primary elections have been re-adapted to different political systems, in order to fit with different party’s specific features. This implied a re-definition of the very concept of primary both in terms of procedures and in terms of political meaning. This paper aims to understand the promotion of party primaries in two EU countries: Italy and France. The two countries have implemented open primaries adopting the maximum level of inclusiveness. This paper will focus on the public debate about these two case studies in order to clarify which were the meaning and the political value of the primary contests in 2011 (France) and 2012 (Italy)

European versus Domestic Politics: Media Negativity during the 2019 European Elections Campaign in Italy

This article aims at understanding to what extent European issues and actors are covered through a negative tone within the news coverage of the 2019 EP election campaign in Italy. We rely on an original dataset based on content analysis of the 50 days prior to the 2019 EP elections in four mainstream newspapers (n=3,943) and four mainstream TV newscasts (n=1,873). Our findings show that the media negativity is primarily associated with domestic politics, while all in all European politics is covered using a less negative tone. This general picture is the results of two differentiated patterns: newspapers are less negative towards both EU politics and Eu and foreign political actors, while TV news are more negative towards Eu and foreign political actors

Alzheimer's Proteins, Oxidative Stress, and Mitochondrial Dysfunction Interplay in a Neuronal Model of Alzheimer's Disease

In this paper, we discuss the interplay between beta-amyloid (Aβ) peptide, Tau fragments, oxidative stress, and mitochondria in the neuronal model of cerebellar granule neurons (CGNs) in which the molecular events reminiscent of AD are activated. The identification of the death route and the cause/effect relationships between the events leading to death could be helpful to manage the progression of apoptosis in neurodegeneration and to define antiapoptotic treatments acting on precocious steps of the death process. Mitochondrial dysfunction is among the earliest events linked to AD and might play a causative role in disease onset and progression. Recent studies on CGNs have shown that adenine nucleotide translocator (ANT) impairment, due to interaction with toxic N-ter Tau fragment, contributes in a significant manner to bioenergetic failure and mitochondrial dysfunction. These findings open a window for new therapeutic strategies aimed at preserving and/or improving mitochondrial function

Transport and metabolism of l-lactate occur in mitochondria from cerebellar granule cells and are modified in cells undergoing low potassium dependent apoptosis

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Cytochrome c is released from mitochondria in a reactive oxygen species (ROS)-dependent fashion and can operate as a ROS scavenger and as a respiratory substrate in cerebellar neurons undergoing excitotoxic death

In rat cerebellar granule cells both reactive oxygen species production and release of cytochrome c take place during glutamate toxicity. This investigation was aimed (i) to ascertain whether and how these two processes are related and (ii) to gain insight into the role played by the released cytochrome c in the onset of neurotoxicity. Cytochrome c release takes place owing to the generation of reactive oxygen species both in glutamate-treated cerebellar granule cells and in sister control cultures incubated in the presence of the reactive oxygen species-generating system consisting of xanthine plus xanthine oxidase. In the early phase of neurotoxicity (30-min glutamate exposure) about 40% of the maximum (as measured at 3 h of glutamate exposure) cytochrome c release was found to occur in cerebellar granule cells from mitochondria that were essentially coupled and intact and that had a negligible production of oxygen free radicals. Contrarily, mitochondria from cells treated with glutamate for 3 h were mostly uncoupled and produced reactive oxygen species at a high rate. The cytosolic fraction containing the released cytochrome c was able to transfer electrons from superoxide anion to molecular oxygen via the respiratory chain and was found to partially prevent glutamate toxicity when added externally to cerebellar neurons undergoing necrosis. In the light of these findings, we propose that in the early phase of neurotoxicity, cytochrome c release can be part of a cellular and mitochondrial defense mechanism against oxidative stress

Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright-Yellow 2 cells

AbstractTo find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS-PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase-3-like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS-PCD upstream of the caspase-like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase-3-like protease activation