WP-108: Enriched personalized multi-screen content for social connected TV

<p>WP-108: Enriched personalized multi-screen content for social connected TV</p

GW4064 treatment inhibited transdifferentiation of HSCs.

<p>Freshly isolated rat HSCs were cultured for 7 days in 10% FCS alone (control) or 10% FCS medium containing 1 µmol/L GW4064. The expression of α-SMA mRNA (A) and protein (B) was examined by real-time RT-PCR and immunofluorescence staining, respectively. Data are mean ±SE of 6 experiments. *<i>P</i><.001 vs. control cells.</p

GW4064 treatment of HSCs led to reduced contractile response to ET-1.

<p>Freshly isolated rat HSCs were cultured for 7 days in 10% FCS medium alone (control) or 10% FCS medium containing 1 µmol/L GW4064. Collagen gel lattices that contained the control or GW4064-treated HSCs were prepared as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013955#s4" target="_blank"><i>Materials & Methods</i></a>. Hydrated collagen lattices were photographed 6 hours after addition of ET-1 (10 nM). Data are mean ±SE of 6 experiments. *<i>P</i><.001 vs. control cells.</p

GW4064 treatment inhibited ET-1-induced Rho-kinase activity in activated HSCs.

<p>Activated HSCs were treated with DMSO or 1 µmol/L GW4064 for 18 hours. ET-1 (10 nM) was then added to the medium, and the cells were harvested 10 min later. Phosphorylation of meosin was detected by Western using appropriate phospho-specific antibody. An immunoblot of total meosin present in the cell extracts is shown as a loading control (lower panel). A representative immunoblot from three independent experiments is presented.</p

GW4064 treatment inhibited ET-1-induced phosphorylation of MLC in activated HSCs.

<p>Activated HSCs were treated with DMSO or 1 µmol/L GW4064 for 18 hours. ET-1 (10 nM) was then added to the medium, and the cells were harvested 10 min later. Phosphorylation of MLC was detected by Western using appropriate phospho-specific antibody. An immunoblot of total MLC present in the cell extracts is shown as a loading control (lower panel). A representative immunoblot from three independent experiments is presented.</p

GW4064 treatment inhibited the upregulation of ET-1 expression during HSC activation.

<p>Freshly isolated rat HSCs were cultured for 7 days in 10% FCS alone (control) or 10% FCS medium containing 1 µmol/L GW4064. The mRNA expression levels of ET-1 (A), ETB (B) and ETA (C) were examined by real-time RT-PCR, respectively. Data are mean ±SE of 6 experiments. *<i>P</i><.001 vs. control cells.</p

Images from different depths on the wall showing qualitative differences with depth, including more sponge cover at the shallowest depth, increased <i>A. excavata</i> and <i>N. zibrowii</i> with depth until the deepest, low biomass, section is reached.

<p>Images from different depths on the wall showing qualitative differences with depth, including more sponge cover at the shallowest depth, increased <i>A. excavata</i> and <i>N. zibrowii</i> with depth until the deepest, low biomass, section is reached.</p

Light attenuation profiles (m⁻¹) in the eastern branch of the Whittard canyon at 375 m (green), 750 m (red), 1000 m (blue) and 1320 m (black) water depth.

<p>Dashed lines (620–740 m) indicate depth of wall where high densities of suspension feeders were found.</p

Location of the <i>A. excavata</i> -<i>N. zibrowii</i> biotope on the southern side of the surveyed canyon (green circle on inlay marked as ‘ROV’).

<p>Deeper waters have cooler colours.</p

Variations in mean <i>A. excavata</i> shell height, <i>A. excavata</i> density and <i>N. zibrowii</i> density as a function of depth.

<p>The range of depths where <i>A. excavata</i> shells were observed on the wall was from 633 to 762 m (open square symbols). Lines are fitted linear regressions except for the panel displaying <i>A. excavata</i> densities where the line is a quantile regression estimating the position of the third quartile (0.75). Quantile regression coefficients were significantly different from zero when tested using bootstrap estimates of SE.</p