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Within-population genetic structure differs between two sympatric sister-species of ectomycorrhizal fungi, Rhizopogon vinicolor and R. vesiculosus
Using spatial autocorrelation analysis, we
examined the within-population genetic structure of
Rhizopogon vinicolor and R. vesiculosus, two hypogeous
ectomycorrhizal (EM) species that are sympatric
sister taxa known to differ in their clonal structure.
We collected 121 sporocarps and 482 tuberculate EM
of both species from a 20 ha forest stand dominated
by Douglas-fir (Pseudotsuga menziesii). Field collections
were identified to species with restriction
fragment length polymorphism analysis of the nuclear
ribosomal internal transcribed spacer. Five and six
microsatellite markers were used to characterize the
genetic diversity of EM and sporocarp samples from
R. vesiculosus and R. vinicolor respectively. After
correcting for genet structure, spatial autocorrelation
analyses of the EM samples were used to test the null
hypothesis that multilocus genotypes characterized
from each species were randomly distributed within
the study area. We detected positive and statistically
significant fine-scale genetic structure up to 120 m
within the R. vesiculosus sample. In contrast, no
spatial genetic structure was evident for R. vinicolor,
indicating that the genotypes characterized for this
species were randomly distributed throughout the
study area. Differences in statistical power or the
nuclear count of basidiospores are unlikely agents of
the genetic patterns observed. Our results suggest
that differences in reproductive output or competitive
ability may act individually or in combination to
create clusters of similar genotypes for R. vesiculosus
throughout the study area.This is the publisher’s final pdf. The published article is copyrighted by the Mycological Society of America and can be found at: http://www.msafungi.org/Keywords: genet, Rhizopogon, ectomycorrhizal, genetic structure, spatial autocorrelation, fung