Production of fermentescible sugar from paper-pulp: looking for a dynamic and multiscale integrated models based on physical parameters

In order to achieve economic viability, the biorefining of lignocellulosic resources must be operated at very high feedstock dry matter content. The paper pulp product is rather appropriate for modern biorefining, because it displays low lignin content, is free of inhibitory compounds that can perturb fermentations and is devoid of microbial contaminants. Nevertheless the enzyme liquefaction and saccharification of paper-like pulps are subject to the same constraints as other pulps obtained via alternative methods such as steam explosion or dilute acid hydrolysis. Therefore, the better scientific understanding and ultimately the technical mastering of these critical biocatalytic reactions, which involve complex matrices at high solids content, is currently a major challenge that must be met in order to facilitate the intensification of biorefining operations. Our aim is to investigate dynamic of transfer phenomena and limitation of biocatalytic reactions with lignocelluloses resources under high concentration conditions. Our action focuses on the identification of rate limiting steps of the liquefactions mechanisms by physical and biochemical characterization of pre-treated lignocellulosic resources at a macroscopic scale (power consumption, rheology), microscopic scale (particle size, morphology) and molecular scale (chemical analysis). Firstly, based on real and model matrices and using Metzner & Reed concept, non-Newtonian rheological behaviour of fiber suspensions are described by a structured rheological model including parameters such as concentration, size and shape. Secondly, the complex relationships between fibre structure, degradation, chemical composition and rheological behaviour is investigated. To this end, physical and biochemical on line and off-line analyses will be conducted during bioreaction with a specific and fully instrumented bioprocess. Relation between apparent viscosity change and biocatalytic degradation of fiber may then be discussed

High-Throughput Functional System for Encapsulated Networks of Model Cell Membranes

Synthetic lipid bilayers provide models of cell membranes to study biomolecular interactions and signal transduction. The droplet interface bilayer (DIB) is a highly versatile technique for assembling planar lipid membranes between water droplets in oil. The DIB method thus provides a unique capability for developing digital, droplet-based membrane platforms for rapid membrane characterization, drug screening and ion channel recordings. In this work, a new microfluidic system is presented that automates droplet generation, sorting, and sequential trapping in designated locations to enable rapid assembly of arrays of DIBs along with in situ electrical measurements. This platform provides repeatable processes for forming long-lasting bilayer arrays for numerous membrane-based applications. Studies on asymmetric lipid membranes are performed to understanding the effects of peptides on the disruption of asymmetric lipid membranes and intramembrane potential. In addition, an automated microfluidic array is applied to isolate and transform single cells to improve the ability to study gene transformation on an individual cell basis, with greater spatial and temporal resolution of each cell’s response

Indiana Biobank (IB)

poster abstractThe Indiana Biobank (IB) was established in July 2010 as a conduit in the new era of personalized medicine to serve the needs of the Indiana research communities and to make an impact on Hoosier health. The overall objective of the Indiana Biobank is to create a collection of high quality biospecimens that are well annotated and linked to the electronic health record, genomic and proteomic data, to provide to the research community to carry out translational research. The ability to successfully do research and translate to the clinical setting is greatly facilitated by the availability of an extensive biorepository of biological samples, with accompanying clinical and genomic data, procured from patients at IU Health, Wishard and other clinical venues throughout Indiana

Mississippi Health and Hunger Atlas 2021

https://egrove.olemiss.edu/libarts_book/1244/thumbnail.jp