Lipophilic and hydrophilic quantitative analysis of antioxidant activity in tomatoes and tomato products

The amount of antioxidants present in food varies depending on environmental conditions in which produce was grown and how products were processed prior to consumption. It would, therefore, be useful to quantify antioxidant activities in these foods. This study focused on quantitative analysis of antioxidant activities in commercially produced whole tomatoes and processed tomato products. For commercially processed tomatoes, diced tomatoes had total antioxidant activities (TAA) ranging from 1.243 to 2.243 μmol TE/g fresh weight (fw), juice 1.573 to 6.86 μmol TE/g fw, paste 6.3 to 13.248 μmol TE/g fw, sauce 1.62 to 3.168 μmol TE/g fw, and soup 1.073 to 3.773 μmol TE/g fw. In commercial whole tomatoes, cherry tomatoes had TAA ranging from 2.303 to 3.66 μmol TE/g fw, grape tomatoes 2.443 to 2.825 μmol TE/g fw, roma tomatoes 0.535 to 3.033 μmol TE/g fw, and slicer tomatoes 1.448 to 2.788 μmol TE/g fw. Variations and significant differences were observed in different samples of the same type of tomatoes, between different types of tomatoes, in different batches of the same brand and kind of processed tomatoes, and between different types of processed tomatoes. These variations could be attributed to the different locales tomatoes were grown, type of tomatoes used for processed tomato products, or additives such as herbs and spices used for flavoring. Additionally, the effect of light intensity on antioxidant accumulation in tomatoes was investigated by experimentally growing plants in different light intensities. Data indicated statistical differences between tomatoes grown under the same as well as different light intensities, on a fresh weight basis. Tomatoes grown in 100% light had TAA ranging from 1.898 to 3.565 μmol TE/g fw (7.73 to 13.405 μmol TE/g dw), 2.375 to 2.523 μmol TE/g fw (8.558 to 13.223 μmol TE/g dw) in 50% light, and 1.623 to 1.958 μmol TE/g fw (8.068 to 13.073 μmol TE/g dw) in 25% light. While small differences in data proved to be statistically significant, some of these differences may be too small to be of biological consequence. Data from this study, along with currently available data on antioxidants in foods, can provide useful information to consumers interested in purchasing products that are most beneficial to their health and to dietitians when making dietary recommendations to patient

Adipocytes, aldosterone and obesity-related hypertension

Understanding the mechanisms linking obesity with hypertension is important in the current obesity epidemic as it may improve therapeutic interventions. Plasma aldosterone levels are positively correlated with body mass index and weight loss in obese patients is reported to be accompanied by decreased aldosterone levels. This suggests a relationship between adipose tissue and the production/secretion of aldosterone. Aldosterone is synthesized principally by the adrenal glands, but its production may be regulated by many factors, including factors secreted by adipocytes. In addition, studies have reported local synthesis of aldosterone in extra-adrenal tissues, including adipose tissue. Experimental studies have highlighted a role for adipocyte-secreted aldosterone in the pathogenesis of obesity-related cardiovascular complications via the mineralocorticoid receptor. This review focuses on how aldosterone secretion may be influenced by adipose tissue and the importance of these mechanisms in the context of obesity-related hypertension

COVID-19 - Revealing Unaddressed Systemic Barriers in the 45th Anniversary of the Southeast Asian American Experience

2020 marks the 45th year anniversary of the Southeast Asian American (SEAA) experience, starting with the first wave of refugees who fled Cambodia, Laos, and Viet Nam as a result of American occupation and wars throughout the region. Collectively, this community is the largest community of refugees ever to be resettled in America. Yet despite four decades in this country, Southeast Asian Americans continue to face disparate challenges like other low-income, immigrant, refugee, communities of color — ranging from poverty, to educational inequity, health disparities, and harsh immigration policies. COVID-19 pandemic has also revealed and exacerbated systemic barriers that have always existed for Southeast Asian American communities. This paper explores those impacts in economic, education, health, and immigration enforcement and concludes with thoughts on the way forward

High-Resolution, Mid-Infrared Color Temperature Mapping of the Central 10 Arcseconds of the Galaxy

The neighborhood of the Galactic black hole boasts a plethora of extended interstellar gas and dust features as well as populations of compact (unresolved, or marginally resolved) features such as the G objects. Most are well manifested in the infrared. To disentangle and characterize the infrared structure of extended features and identify compact sources, we used 3.8~$\mu$m (L' filter) data from the NIRC2 imager at the Keck Observatory and 8.6~$\mu$m (PAH1 filter) data from the VISIR imager at the Very Large Telescope (VLT) to produce the highest-resolution mid-IR color-temperature map of the inner half-parsec of the Galactic Center to date. From this map, we compile a catalog of features that stand out from their background. In particular, we identify 33 compact sources that stand out against the local background temperature, 11 of which are newly identified and are candidates for being members of the G objects population. Additionally, we resolve and newly characterize the morphology of several known extended features. These results prepare the way for ongoing and future JWST studies that have access to a greater range of mid-infrared wavelengths, and thus will allow for refined estimation of the trends of dust temperatures.Comment: 22 pages, 14 figure

Yes, In My Backyard: Building ADUs to Address Homelessness

Several cities around the country are experimenting with accessory dwellings – small units in residential backyards – to address housing shortages and homelessness crises. This guide analyzes innovative case studies in Colorado, Washington, California, and Oregon to provide lessons on structural design, project funding, screening and matching residents and hosts, potential legal liabilities, zoning regulations, and public relations considerations

Trend of Outcome Metrics in Recent Out-of-Hospital-Cardiac-Arrest Research: A Narrative Review of Clinical Trials

Cardiopulmonary resuscitation (CPR) research traditionally focuses on survival. In 2018, the International Liaison Committee on Resuscitation (ILCOR) proposed more patient-centered outcomes. Our narrative review assessed clinical trials after 2018 to identify the trends of outcome metrics in the field OHCA research. We performed a search of the PubMed database from 1 January 2019 to 22 September 2023. Prospective clinical trials involving adult humans were eligible. Studies that did not report any patient-related outcomes or were not available in full-text or English language were excluded. The articles were assessed for demographic information and primary and secondary outcomes. We included 89 studies for analysis. For the primary outcome, 31 (35%) studies assessed neurocognitive functions, and 27 (30%) used survival. For secondary outcomes, neurocognitive function was present in 20 (22%) studies, and survival was present in 10 (11%) studies. Twenty-six (29%) studies used both survival and neurocognitive function. Since the publication of the COSCA guidelines in 2018, there has been an increased focus on neurologic outcomes. Although survival outcomes are used frequently, we observed a trend toward fewer studies with ROSC as a primary outcome. There were no quality-of-life assessments, suggesting a need for more studies with patient-centered outcomes that can inform the guidelines for cardiac-arrest management

High-throughput biodiversity analysis: Rapid assessment of species richness and ecological interactions of Chrysomelidae (Coleoptera) in the tropics

Biodiversity assessment has been the focus of intense debate and conceptual and methodological advances in recent years. The cultural, academic and aesthetic impulses to recognise and catalogue the diversity in our surroundings, in this case of living objects, is furthermore propelled by the urgency of understanding that we may be responsible for a dramatic reduction of biodiversity, comparable in magnitude to geological mass extinctions. One of the most important advances in this attempt to characterise biodiversity has been incorporating DNA-based characters and molecular taxonomy tools to achieve faster and more efficient species delimitation and identification, even in hyperdiverse tropical biomes. In this assay we advocate for a broad understanding of Biodiversity as the inventory of species in a given environment, but also the diversity of their interactions, with both aspects being attainable using molecular markers and phylogenetic approaches. We exemplify the suitability and utility of this framework for large-scale biodiversity assessment with the results of our ongoing projects trying to characterise the communities of leaf beetles and their host plants in several tropical setups. Moreover, we propose that approaches similar to ours, establishing the inventories of two ecologically inter-related and species-rich groups of organisms, such as insect herbivores and their angiosperm host-plants, can serve as the foundational stone to anchor a comprehensive assessment of diversity, also in tropical environments, by subsequent addition of trophic levels.The ‘Fundación BBVA’ (Spain) has funded the bulk of this work thanks to their support for our large-scale biodiversity assessment initiative in Nicaraguan tropical dry forests (project BIOCON08-045, IP: JGZ). Our work in Nicaragua has benefited from a postdoctoral ‘Juan de la Cierva’ contract (Spanish Ministry of Science and Innovation, MICINN) to AP, and an AECID predoctoral studentship (Spanish Ministry of Foreign Affairs and Cooperation) and a SENESCYT scholarship (Secretariat of High Education, Science, Technology and Innovation, Ecuador) to GDC. The National Geographic Society supported most of our research in New Caledonia (project 8380-07, IP: JGZ) with help from a travel grant awarded by the Percy Sladen Memorial Fund of the Linnean Society of London to JGZ. The Spanish High Research Council (CSIC), in the framework of a cooperation agreement with the Vietnamese Academy of Sciences, supports our work in dry tropical forests of southern Vietnam (IP: JGZ) as well as a predoctoral studentship to DTN. Several EU Synthesys research stays (GB-TAF-1840, SE-TAF-1893, DE-TAF-4348) and a Mayr Travel Grant (Harvard University) as well as project CGL2008-00007/BOS (MICINN, IP: JGZ) have contributed to the discovery of a new tropical species of Calligrapha, and the latter also framed the predoctoral studentship to TM.Peer Reviewe

A mock circulation loop to test extracorporeal CO2 elimination setups

Background: Extracorporeal carbon dioxide removal (ECCO2R) is a promising yet limited researched therapy for hypercapnic respiratory failure in acute respiratory distress syndrome and exacerbated chronic obstructive pulmonary disease. Herein, we describe a new mock circuit that enables experimental ECCO2R research without animal models. In a second step, we use this model to investigate three experimental scenarios of ECCO2R: (I) the influence of hemoglobin concentration on CO2 removal. (II) a potentially portable ECCO2R that uses air instead of oxygen, (III) a low-flow ECCO2R that achieves effective CO2 clearance by recirculation and acidification of the limited blood volume of a small dual lumen cannula (such as a dialysis catheter). Results: With the presented ECCO2R mock, CO2 removal rates comparable to previous studies were obtained. The mock works with either fresh porcine blood or diluted expired human packed red blood cells. However, fresh porcine blood was preferred because of better handling and availability. In the second step of this work, hemoglobin concentration was identified as an important factor for CO2 removal. In the second scenario, an air-driven ECCO2R setup showed only a slightly lower CO2 wash-out than the same setup with pure oxygen as sweep gas. In the last scenario, the low-flow ECCO2R, the blood flow at the test membrane lung was successfully raised with a recirculation channel without the need to increase cannula flow. Low recirculation ratios resulted in increased efficiency, while high recirculation ratios caused slightly reduced CO2 removal rates. Acidification of the CO2 depleted blood in the recirculation channel caused an increase in CO2 removal rate. Conclusions: We demonstrate a simple and cost effective, yet powerful, “in-vitro” ECCO2R model that can be used as an alternative to animal experiments for many research scenarios. Moreover, in our approach parameters such as hemoglobin level can be modified more easily than in animal models

A Novel Mock Circuit to Test Full-Flow Extracorporeal Membrane Oxygenation

Extracorporeal membrane oxygenation (ECMO) has become an important therapeutic approach in the COVID-19 pandemic. The development and research in this field strongly relies on animal models; however, efforts are being made to find alternatives. In this work, we present a new mock circuit for ECMO that allows measurements of the oxygen transfer rate of a membrane lung at full ECMO blood flow. The mock utilizes a large reservoir of heparinized porcine blood to measure the oxygen transfer rate of the membrane lung in a single passage. The oxygen transfer rate is calculated from blood flow, hemoglobin value, venous saturation, and post-membrane arterial oxygen pressure. Before the next measuring sequence, the blood is regenerated to a venous condition with a sweep gas of nitrogen and carbon dioxide. The presented mock was applied to investigate the effect of a recirculation loop on the oxygen transfer rate of an ECMO setup. The recirculation loop caused a significant increase in post-membrane arterial oxygen pressure (paO2 ). The effect was strongest for the highest recirculation flow. This was attributed to a smaller boundary layer on gas fibers due to the increased blood velocity. However, the increase in paO2 did not translate to significant increases in the oxygen transfer rate because of the minor significance of physically dissolved oxygen for gas transfer. In conclusion, our results regarding a new ECMO mock setup demonstrate that recirculation loops can improve ECMO performance, but not enough to be clinically relevant

Comparison of Circular and Parallel-Plated Membrane Lungs for Extracorporeal Carbon Dioxide Elimination

Extracorporeal carbon dioxide removal (ECCO2R) is an important technique to treat critical lung diseases such as exacerbated chronic obstructive pulmonary disease (COPD) and mild or moderate acute respiratory distress syndrome (ARDS). This study applies our previously presented ECCO2R mock circuit to compare the CO2 removal capacity of circular versus parallel-plated membrane lungs at different sweep gas flow rates (0.5, 2, 4, 6 L/min) and blood flow rates (0.3 L/min, 0.9 L/min). For both designs, two low-flow polypropylene membrane lungs (Medos Hilte 1000, Quadrox-i Neonatal) and two mid-flow polymethylpentene membrane lungs (Novalung Minilung, Quadrox-iD Pediatric) were compared. While the parallel-plated Quadrox-iD Pediatric achieved the overall highest CO2 removal rates under medium and high sweep gas flow rates, the two circular membrane lungs performed relatively better at the lowest gas flow rate of 0.5 L/min. The low-flow Hilite 1000, although overall better than the Quadrox i-Neonatal, had the most significant advantage at a gas flow of 0.5 L/min. Moreover, the circular Minilung, despite being significantly less efficient than the Quadrox-iD Pediatric at medium and high sweep gas flow rates, did not show a significantly worse CO2 removal rate at a gas flow of 0.5 L/min but rather a slight advantage. We suggest that circular membrane lungs have an advantage at low sweep gas flow rates due to reduced shunting as a result of their fiber orientation. Efficiency for such low gas flow scenarios might be relevant for possible future portable ECCO2R devices