Behavioural and physiological adaptations to low-temperature environments in the common frog, Rana temporaria

<b>Background</b><p></p> Extreme environments can impose strong ecological and evolutionary pressures at a local level. Ectotherms are particularly sensitive to low-temperature environments, which can result in a reduced activity period, slowed physiological processes and increased exposure to sub-zero temperatures. The aim of this study was to assess the behavioural and physiological responses that facilitate survival in low-temperature environments. In particular, we asked: 1) do high-altitude common frog (Rana temporaria) adults extend the time available for larval growth by breeding at lower temperatures than low-altitude individuals?; and 2) do tadpoles sampled from high-altitude sites differ physiologically from those from low-altitude sites, in terms of routine metabolic rate (RMR) and freeze tolerance? Breeding date was assessed as the first day of spawn observation and local temperature recorded for five, paired high- and low-altitude R. temporaria breeding sites in Scotland. Spawn was collected and tadpoles raised in a common laboratory environment, where RMR was measured as oxygen consumed using a closed respiratory tube system. Freeze tolerance was measured as survival following slow cooling to the point when all container water had frozen.<p></p> <b>Results</b><p></p> We found that breeding did not occur below 5°C at any site and there was no significant relationship between breeding temperature and altitude, leading to a delay in spawning of five days for every 100 m increase in altitude. The relationship between altitude and RMR varied by mountain but was lower for individuals sampled from high- than low-altitude sites within the three mountains with the highest high-altitude sites (≥900 m). In contrast, individuals sampled from low-altitudes survived freezing significantly better than those from high-altitudes, across all mountains.<p></p> <b>Conclusions</b><p></p> Our results suggest that adults at high-altitude do not show behavioural adaptations in terms of breeding at lower temperatures. However, tadpoles appear to have the potential to adapt physiologically to surviving at high-altitude via reduced RMR but without an increase in freeze tolerance. Therefore, survival at high-altitude may be facilitated by physiological mechanisms that permit faster growth rates, allowing completion of larval development within a shorter time period, alleviating the need for adaptations that extend the time available for larval growth

The health effects of air pollution in Delhi, India

The authors report the results of a time-series study of the impact of particulate air pollution on daily mortality in Delhi. They find: a) A positive, significant relationship between particulate pollution and daily nontraumatic deaths as well as deaths from certain causes (respiratory and cardiovascular problems) and for certain age groups. b) In general, these impacts are smaller than those estimated for other countries, where on average a 100-microgram increase in total suspended particulates (TSP) leads to a 6-percent increase in nontraumatic mortality. In Delhi, such an increase in TSP is associated with a 2.3-percent increase in deaths. c) The differences in magnitudes of the effects are most likely explained by differences in distributions of age at death and cause of death, as most deaths in Delhi occur before the age of 65 and are not attributed to causes with a strong association with air pollution. d) Although air pollution seems to have less impact on mortality counts in Delhi, the number of life-years saved per death avoided is greater in Delhi than in US cities -- because the age distribution of impacts in these two places varies. In the United States particulates have the greatest influence on daily deaths among persons 65 and older. In Delhi, they have the greatest impact in the 15-to-44 age group. That means that for each death associated with air pollution, on average more life-years would be saved in Delhi than in the United States. Large differences in the magnitude of effects do call into question the validity of the"concentration-response transfer"procedure. In that procedure, concentration-response relationships found for industrial countries are applied to cities in developing countries with little or no adjustment, to estimate the effects of pollution on daily mortality.Demographics,Public Health Promotion,Montreal Protocol,Health Monitoring&Evaluation,Air Quality&Clean Air,Health Monitoring&Evaluation,Montreal Protocol,Demographics,Environmental Economics&Policies,Health Systems Development&Reform

Monodisperse self-assembly in a model with protein-like interactions

We study the self-assembly behaviour of patchy particles with `protein-like' interactions that can be considered as a minimal model for the assembly of viral capsids and other shell-like protein complexes. We thoroughly explore the thermodynamics and dynamics of self assembly as a function of the parameters of the model and find robust assembly of all target structures considered. Optimal assembly occurs in the region of parameter space where a free energy barrier regulates the rate of nucleation, thus preventing the premature exhaustion of the supply of monomers that can lead to the formation of incomplete shells. The interactions also need to be specific enough to prevent the assembly of malformed shells, but whilst maintaining kinetic accessibility. Free-energy landscapes computed for our model have a funnel-like topography guiding the system to form the target structure, and show that the torsional component of the interparticle interactions prevents the formation of disordered aggregates that would otherwise act as kinetic traps.Comment: 11 pages; 10 figure

Reductio ad absurdum como estratégia argumentativa. Um estudo de caso

Nos argumentos darwinianos, há uma tácita suposição da "explicabilidade" racional dos fenômenos naturais. A essa tarefa explicativa, em situações ejemplares da Origem das Espécies como a do argumento a favor da origem comum das raças de pombo doméstico (cap. I), a ser presentemente examinado, a reductio ad absurdum reveste-se de um alcance estratégico que excede o de mera aplicão de urna regra lógica inferencial e garante a "explicabilidade" dos fatos. Esse modo de argumentar serve de modo bastante eficaz áquelas suposições -de resto tão comuns ao raciocínio filosófico, como nos diz Ryle- em que carecemos do que seria urna "prova" direta a favor de urna determinada hipótese

O “AVESSO” E O “DIREITO” EM BUSCA DA RACIONALIDADE DA CIÊNCIA

Tradicionalmente, “racionalidade” é o exercício da faculdade superior de
 conhecimento e ação, “razão”, e a propriedade dos produtos resultantes desse exercício, “razões”. Buscar o significado da “racionalidade” pressupõe perscrutá-la “em ação” – não há como “sair fora” do racional para encontrá-la. O valor de face que apresenta é o lado “direito” de sua tessitura. Mas a condição (auto)reguladora da racionalidade demanda que
 se lhe busque também o “avesso”, lado nem sempre visível das costuras que dão a feitura do “direito”. Em face da contextualidade da racionalidade “em ação”, cabe examinar essa distinção através de dois “casos exemplares”: a visão aristotélica de racionalidade e a argumentação de Charles Darwin na Origem das espécies. De um lado, encontramos uma
 outra vertente da Retórica aristotélica, mostrando a interpenetração de demonstrar e persuadir no “avesso” da racionalidade; de outro, encontramos no “avesso” da argumentação científica darwiniana a essencialidade de procedimentos ditos “retóricos”. Cruzam-se as fronteiras.
 
 Palavras-chave: Racionalidade, argumentação, retórica, ciência, Aristóteles, Charles Darwin

Thurstonian Scaling of Compositional Questionnaire Data

To prevent response biases, personality questionnaires may use comparative response formats. These include forced choice, where respondents choose among a number of items, and quantitative comparisons, where respondents indicate the extent to which items are preferred to each other. The present article extends Thurstonian modeling of binary choice data (Brown & Maydeu-Olivares, 2011a) to “proportion-of-total” (compositional) formats. Following Aitchison (1982), compositional item data are transformed into log-ratios, conceptualized as differences of latent item utilities. The mean and covariance structure of the log-ratios is modelled using Confirmatory Factor Analysis (CFA), where the item utilities are first-order factors, and personal attributes measured by a questionnaire are second-order factors. A simulation study with two sample sizes, N=300 and N=1000, shows that the method provides very good recovery of true parameters and near-nominal rejection rates. The approach is illustrated with empirical data from N=317 students, comparing model parameters obtained with compositional and Likert scale versions of a Big Five measure. The results show that the proposed model successfully captures the latent structures and person scores on the measured traits

Development and validation of the guideline for reporting evidence-based practice educational interventions and teaching (GREET)

Abstract Background The majority of reporting guidelines assist researchers to report consistent information concerning study design, however, they contain limited information for describing study interventions. Using a three-stage development process, the Guideline for Reporting Evidence-based practice Educational interventions and Teaching (GREET) checklist and accompanying explanatory paper were developed to provide guidance for the reporting of educational interventions for evidence-based practice (EBP). The aim of this study was to complete the final development for the GREET checklist, incorporating psychometric testing to determine inter-rater reliability and criterion validity. Methods The final development for the GREET checklist incorporated the results of a prior systematic review and Delphi survey. Thirty-nine items, including all items from the prior systematic review, were proposed for inclusion in the GREET checklist. These 39 items were considered over a series of consensus discussions to determine the inclusion of items in the GREET checklist. The GREET checklist and explanatory paper were then developed and underwent psychometric testing with tertiary health professional students who evaluated the completeness of the reporting in a published study using the GREET checklist. For each GREET checklist item, consistency (%) of agreement both between participants and the consensus criterion reference measure were calculated. Criterion validity and inter-rater reliability were analysed using intra-class correlation coefficients (ICC). Results Three consensus discussions were undertaken, with 14 items identified for inclusion in the GREET checklist. Following further expert review by the Delphi panelists, three items were added and minor wording changes were completed, resulting in 17 checklist items. Psychometric testing for the updated GREET checklist was completed by 31 participants (n = 11 undergraduate, n = 20 postgraduate). The consistency of agreement between the participant ratings for completeness of reporting with the consensus criterion ratings ranged from 19 % for item 4 Steps of EBP, to 94 % for item 16 Planned delivery. The overall consistency of agreement, for criterion validity (ICC 0.73) and inter-rater reliability (ICC 0.96), was good to almost perfect. Conclusion The final GREET checklist comprises 17 items which are recommended for reporting EBP educational interventions. Further validation of the GREET checklist with experts in EBP research and education is recommended

Conflict of Laws (2004)

States\u27 and nations\u27 laws collide when foreign factors appear in a lawsuit. Nonresident litigants, incidents outside the forum, parallel lawsuits, and judgments from other jurisdictions can create problems with personal jurisdiction, choice of law, and the recognition of foreign judgments. This article reviews Texas conflicts cases from Texas state and federal courts during the Survey period from October 1, 2002, through November 1, 2003. The article excludes cases involving federalstate conflicts, intrastate issues such as subject matter jurisdiction and venue, and conflicts in time, such as the applicability of prior or subsequent law within a state. State and federal cases are discussed together because conflict of laws is mostly a state law topic, except for a few constitutional limits, resulting in the same rules applying to most issues in state and federal courts. The Survey period saw an expansive litigation of forum contests, including nine cases based on agency, alter ego, or corporate relationship. In choice of law, the United States Supreme Court issued what may be its most important ruling in fifty years, holding that the forum state may apply its own law without balancing the competing interests of other states, diminishing the constitutional role of interest analysis in choice of law. Texas state and federal courts continued their development of the most significant relationship test, including an unusual application of choice of law to fraudulent joinder in a federal diversity case. Foreign judgments cases followed a similar pattern of routine application of the uniform acts, but with instructive holdings in several areas of commercial litigation, arbitration and family law

Conflict of Laws (2003)

States\u27 and nations\u27 laws collide when foreign factors appear in a lawsuit. Nonresident litigants, incidents outside the forum, parallel lawsuits, and judgments from other jurisdictions can create problems with personal jurisdiction, choice of law, and the recognition of foreign judgments. This article reviews Texas conflicts cases from Texas state and federal courts during the Survey period from October 1, 2001, through November 1, 2002. The article excludes cases involving federal-state conflicts, intrastate issues such as subject matter jurisdiction and venue, and conflicts in time, such as the applicability of prior or subsequent law within a state. State and federal cases are discussed together because conflicts of laws is mostly a state law topic, except for a few constitutional limits, resulting in the same rules applying to most issues in state and federal courts. The discussion is organized according to conflict of laws categories. For jurisdiction over nonresidents, the categories are the grounds for amenability-consent, forum contacts, and grounds for declining jurisdiction. The choice of law categories reflect the hierarchy of choice of law rules, first statutory, then party choice of law, then the Restatement (Second)\u27s most-significant-relationship test, followed by miscellaneous issues such as constitutional limits, proof of foreign law, and limitations. The foreign judgments categories are enforcement (according to specific uniform acts) and preclusion (interstate and international). During the Survey period, forum contests included a variety of jurisdictional assertions over nonresidents in contract, tort and other settings. Jurisdictional theories included a service-of-suit clause construed as consent to amenability, alter-ego and the single-enterprise doctrine, and cases exploring the boundaries of general jurisdiction (such as jurisdiction based on unrelated banking activity). Jurisdiction was lacking over a Belgian employment law claim, and a nationwide federal long-arm failed because of a predicate venue provision. Courts reached opposite results in two internet cases, discussed retained jurisdiction under the new child custody act, and issued an unauthorized anti-suit injunction against a Mississippi lawsuit. Choice of law cases affirmed the parties\u27 right to choose their governing law, declined to adopt the Restatement\u27s statute of limitations rule, upheld arbitration agreements but strictly construed them to exclude children not subject to the contract, applied Texas insurance law--the insurable interest doctrine--to several companies\u27 purchase of life insurance policies on Texas employees (and considered the constitutionality given the case\u27s contacts with eight states), reiterated the requirements for proof of foreign law, and applied Texas\u27s new borrowing statute to an asbestosis claim arising in Alaska. Foreign judgments cases discussed the difference between jurisdictional facts and the merits in a jurisdictional challenge to a New Jersey judgment, upheld the validity of English due process, and found the Uniform Foreign Country Money Judgment Recognition Act persuasive in a preclusion case rejecting a non-monetary Mexican judgment