Biodiesel Production From Lignocellulosic Biomass Using Oleaginous Microbes: Prospects for Integrated Biofuel Production

Biodiesel is an eco-friendly, renewable, and potential liquid biofuel mitigating greenhouse gas emissions. Biodiesel has been produced initially from vegetable oils, non-edible oils, and waste oils. However, these feedstocks have several disadvantages such as requirement of land and labor and remain expensive. Similarly, in reference to waste oils, the feedstock content is succinct in supply and unable to meet the demand. Recent studies demonstrated utilization of lignocellulosic substrates for biodiesel production using oleaginous microorganisms. These microbes accumulate higher lipid content under stress conditions, whose lipid composition is similar to vegetable oils. In this paper, feedstocks used for biodiesel production such as vegetable oils, non-edible oils, oleaginous microalgae, fungi, yeast, and bacteria have been illustrated. Thereafter, steps enumerated in biodiesel production from lignocellulosic substrates through pretreatment, saccharification and oleaginous microbe-mediated fermentation, lipid extraction, transesterification, and purification of biodiesel are discussed. Besides, the importance of metabolic engineering in ensuring biofuels and biorefinery and a brief note on integration of liquid biofuels have been included that have significant importance in terms of circular economy aspects.Fil: Chintagunta, Anjani Devi. Vignan’s Foundation for Science, Technology and Research. Department of Biotechnology; IndiaFil: Zuccaro, Gaetano. Institut National de la Recherche Agronomique; Francia. Università degli Studi di Napoli Federico II; ItaliaFil: Kumar, Mahesh. Central Agricultural University; IndiaFil: Kumar, S. P. Jeevan. Indian Institute of Seed Science; India. Directorate of Floricultural Research; IndiaFil: Garlapati, Vijay Kumar. Jaypee University of Information Technology; IndiaFil: Postemsky, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Kumar, N. S. Sampath. Vignan’s Foundation for Science, Technology and Research. Department of Biotechnology; IndiaFil: Chandel, Anuj K.. Universidade de Sao Paulo; BrasilFil: Simal Gandara, Jesus. Universidad de Vigo; Españ

Laccase mediated delignification of pineapple leaf waste: an ecofriendly sustainable attempt towards valorization

Abstract Background Escalating energy security, burgeoning population and rising costs of fossil fuels have focussed our attention on tapping renewable energy sources. As the utilization of food crops for biofuel production culminates into food vs. fuel dilemma, there is an intensive need for alternatives. Production of biofuels from lignocellulosic biomass owing to its profuse availability and high holocellulose content is a promising area for research. Results In the present study, pineapple leaf, an agro-industrial waste was pretreated with laccase to enhance the enzymatic digestibility of the substrate for improved production of reducing sugar. Variables determining enzymatic delignification of pineapple leaf waste have been optimized by response surface methodology based on central composite design. Maximum delignification of 78.57%(w/w) resulted in reducing sugar of 492.33 ± 3.1 mg/g in 5.30 h. The structural changes in pineapple leaf waste, after laccase treatment, were studied through Fourier transformed infrared spectroscopy, X-ray diffraction and Scanning electron microscopy. Specific surface area, pore volume, and pore diameter of the substrate were studied using the Brunauer–Emmett–Teller and Barrett–Joyner–Halenda methods and found a significant increase in the aforementioned parameters after delignification. Conclusion Laccase mediated delignification of pineapple leaf waste is a cleaner sustainable process for enhanced production of reducing sugar which can accomplish the demand for biofuels

Non-thermal plasmas for disease control and abiotic stress management inplants

National audienceClimate change is predicted to cause severe loss in agricultural production by increasing disease epidemics and intensifying abiotic stresses. Therefore, there is a need for sustainable methods to alleviate plant stress, such as non-thermal plasma. Here we review the role of non-thermal plasma for plant treatment, with focus on the control of viruses, bacteria, fungi and other diseases. We present factors influencing the microbicidal activity of non-thermal plasma. Application of non-thermal plasma for combating abiotic stresses such as drought, metal toxicity, nanoparticles and salinity are discussed. Plasma-generated reactive species trigger the activity of stress-responsive genes in plants. The hypothetical mechanisms involved in triggering the activity of different stress-responsive genes controlling diseases as well as abiotic stresses, are also presented and discussed. The mechanism of plant-plasma interaction is similar to priming, hormesis or adaptive response, and resembles vaccination in animals and humans

Implications of reactive oxygen and nitrogen species in seed physiology for sustainable crop productivity under changing climate conditions

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) incessantly produced as by-products of metabolism play significant roles in seed physiology. ROS (hydroxyl, superoxide radical and hydrogen peroxide) and RNS (nitric oxide, nitric dioxide, nitrous acid and dinitrogen tetroxide) content changes in all phases of seed life cycle that influence seed germination, dormancy and longevity. Recent studies illustrate that ROS and RNS are performing oxidative and nitrosative signaling to induce seed germination within oxidative window level. Besides, ROS/RNS-mediated post-translational modifications (PTM) like carbonylation, S-nitrosylation and nitration are gaining interest in promoting seed germination. Understanding the signalling pathways, cross-talk with plant hormones and their role in promoting seed germination and dormancy alleviation could pave way for hormone engineering that help in crop productivity, particularly under climatic changing conditions. In addition, role of antioxidants and glutathione thiols in protecting from oxidative damage indicate that these compounds can be used for seed viability/quality markers that aid in monitoring of crop establishment. In this review, sources of ROS and RNS, their cross-talk with plant hormones (prospects for hormone engineering), signalling functions pertaining to seed germination, dormancy and deterioration have been illustrated. In addition, seed quality markers under climatic changing conditions for effective monitoring of crop stand establishment and diagnostics development have been elucidated.Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGSaclay Plant Sciences | Ref. ANR-17-EUR-000