Retrospektive Bestimmung der Prävalenz Influenzavirus-spezifischer IgG-Antikörper bei Kindern und Jugendlichen im Alter von 0 bis 18 Jahren

In diese Untersuchung wurden 1.111 Seren von Kindern und Jugendlichen im Alter zwischen 0-18 Jahren einbezogen. Als Vergleichsgruppe diente eine Gruppe gesunder Blutspender im Alter zwischen 18-30 Jahren (809 Proben). Methodisch wurde die indirekte ELISA-Technik (Antigen: influenzavirus-spezifische Ribonukleo- und Matrixproteine) eingesetzt. Die Proben wurden mit dem Influenzavirus A/B IgG-ELISA der Firma Nova-Tec-Immundiagnostica GmbH untersucht. FAZIT: Nach dem Abbau der mütterlichen Leihantikörper im zweiten Lebenshalbjahr wurde ein Anstieg der Antikörper (AK)-Prävalenzen nachgewiesen. Infektionen mit dem Influenzavirus A setzen im frühen Kindesalter ein. Fast alle Kinder > 9 Jahre hatten bereits Kontakt mit dem Influenzavirus A (Prävalenz bis 100% bei den 12-15 Jährigen). Die Gesamtprävalenz der IgG-AK gegen Influenzavirus A betrug 82% bei den Kindern und 99,4% bei den Erwachsenen. Infektionen mit Influenzavirus B erfolgen später. Bis zum 12. Lebensjahr weisen fast alle Kinder keine Antikörper gegen Influenzavirus B auf (12-15 Jahre: 24%). Die Gesamtprävalenz der IgG-AK gegen Influenzavirus B betrug 9,6% bei den Kindern und 56,7% bei den Erwachsenen. Eine jährliche Influenzaschutz-Impfung bei Kindern und Jugendlichen könnte den Schutz vor einer Influenza-Infektion bei epidemiologischen Ausbrüchen verbessern.In this study sera of 1111 children and adolescents aged 0-18 years were included. As a comparison group was a group of healthy blood donors aged 18-30 years (809 samples). Methodologically the indirect ELISA technique (Antigen: influenza virus-specific ribo-and matrix proteins) was used. The samples were analyzed with the influenza virus A / B IgG-ELISA, the company Nova-Tec Immundiagnostica GmbH. CONCLUSION: After the reduction of maternal Leihantikörper in the second half of life has been a rise in antibody (AK)-prevalence demonstrated. Infection with the influenza A virus use in early childhood. Almost all children> 9 years had been in contact with the influenza A virus (prevalence of up to 100% for 12-15 year olds). The overall prevalence of IgG antibodies to influenza virus A was 82% among children and 99.4% for adults. Infection with influenza virus B shall be made later. By 12 Age, almost all children, no antibodies against influenza virus B (12-15 years: 24%). The overall prevalence of IgG antibodies to influenza virus B was 9.6% among children and 56.7% for adults. An annual influenza vaccine protection in children and adolescents could enhance protection against influenza infection in epidemiological outbreaks

Absence of vaccine-enhanced RSV disease and changes in pulmonary dendritic cells with adenovirus-based RSV vaccine

The development of a vaccine against respiratory syncytial virus (RSV) has been hampered by the risk for vaccine-enhanced RSV pulmonary disease induced by immunization with formalin-inactivated RSV (FIRSV). This study focuses on the evaluation of vaccine-enhanced pulmonary disease following immunization with AdF.RGD, an integrin-targeted adenovirus vector that expresses the RSV F protein and includes an RGD (Arg-Gly-Asp) motif. Immunization of BALB/c mice with AdF.RGD, resulted in anti-RSV protective immunity and induced increased RSV-specific IFN-γ T cell responses compared to FIRSV. RSV infection 5 wk after immunization with FIRSV induced pulmonary inflammatory responses in the lung, that was not observed with AdF.RGD. Additionally, In the FIRSV-immunized mice following infection with RSV, pulmonary DC increased and Tregs decreased. This suggests that distinct responses of pulmonary DC and Tregs are a features of vaccine-enhanced RSV disease and that immunization with an RGD-modified Ad vaccine does not trigger vaccine-enhanced disease

Study of Effector CD8+ T Cell Interactions with Cortical Neurons in Response to Inflammation in Mouse Brain Slices and Neuronal Cultures

Cytotoxic CD8+ T cells contribute to neuronal damage in inflammatory and degenerative CNS disorders, such as multiple sclerosis (MS). The mechanism of cortical damage associated with CD8+ T cells is not well understood. We developed in vitro cell culture and ex vivo brain slice co-culture models of brain inflammation to study CD8+ T cell–neuron interactions. To induce inflammation, we applied T cell conditioned media, which contains a variety of cytokines, during CD8+ T cell polyclonal activation. Release of IFNγ and TNFα from co-cultures was verified by ELISA, confirming an inflammatory response. We also visualized the physical interactions between CD8+ T cells and cortical neurons using live-cell confocal imaging. The imaging revealed that T cells reduced their migration velocity and changed their migratory patterns under inflammatory conditions. CD8+ T cells increased their dwell time at neuronal soma and dendrites in response to added cytokines. These changes were seen in both the in vitro and ex vivo models. The results confirm that these in vitro and ex vivo models provide promising platforms for the study of the molecular details of neuron–immune cell interactions under inflammatory conditions, which allow high-resolution live microscopy and are readily amenable to experimental manipulation

Nutrient-dense crops for rural and peri-urban smallholders in Kenya-A regional social accounting approach

Kenya ranks among the countries with the highest micronutrient deficiency worldwide. Due to their high micronutrient content, African indigenous vegetables (AIVs) can be a solution to this problem, and urban areas in Kenya have seen a rise in demand for these crops in the previous decade. To fill the gap between supply and demand, programmes to promote AIV production have been implemented in rural and peri-urban areas. However, the effects of increased AIV production on income and food security in the regional economies are not clear. Thus, in this analysis, we first evaluate differences between the livelihoods of household groups with different levels of food security in rural and peri-urban Kenya using a two-step cluster analysis. Then, we generate a regional social accounting matrix (SAM) and calculate the direct and indirect income effects of AIVs and other crops grown in the area using a multiplier analysis. For the analysis, a total of 706 small-scale vegetable producers in four counties in Kenya were interviewed in 2015. Households in rural areas were more food insecure, especially with respect to the utilization and stability dimension of food security. Multiplier analysis showed increased indirect income effects of AIVs in the regional economy compared to those of many cash crops. We suggest further promoting the production of AIVs in rural and peri-urban Kenya

Governance and stakeholder perspectives of managed re-alignment: Adapting to sea level rise in the Inner Forth estuary, Scotland

With climate change, coastal areas are faced with unprecedented sea level rise and flooding, raising questions as to how societies will choose to adapt. One option is to strengthen existing sea walls to maintain current land uses; however, scientists, policy-makers and conservationists increasingly see the benefits of managed realignment, which is a nature-based coastal adaptation that involves the conversion of reclaimed farmland back to wetlands, allowing periodic local flooding in designated areas to reduce the risk of flooding downstream. We interviewed 16 local organisations, landowners and farmers and held workshops with 109 citizens living the Inner Forth estuary in eastern Scotland, to examine how managed realignment is supported by stakeholder attitudes and their engagement. Most of the farmers we interviewed prefer strengthened sea walls, to maintain their livelihoods and agricultural heritage. Citizens and local organisations were mainly supportive of managed realignment, because it provided wildlife and flood regulation benefits. However, we identified several barriers that could present obstacles to implementing managed realignment, for example, uncertainty whether it would support their principles of economic and rational decision-making. Our findings suggest that the local capacity to cope with rising sea levels is limited by lack of engagement with all relevant stakeholder groups, the limited scope of existing stakeholder partnerships and poor short-term funding prospects of landscape partnerships that would facilitate collaboration and discussion. We suggest that including citizens, landowners, farmers and industries would strengthen existing stakeholder deliberation and collaboration, and support the Inner Forth’s transition towards a more sustainable future shoreline

Cancer Patient T Cells Genetically Targeted to Prostate-Specific Membrane Antigen Specifically Lyse Prostate Cancer Cells and Release Cytokines in Response to Prostate-Specific Membrane Antigen

AbstractThe expression of immunoglobulin-based artificial receptors in normal T lymphocytes provides a means to target lymphocytes to cell surface antigens independently of major histocompatibility complex restriction. Such artificial receptors have been previously shown to confer antigen-specific tumoricidal properties in murine T cells. We constructed a novel ζ chain fusion receptor specific for prostate-specific membrane antigen (PSMA) termed Pz-1. PSMA is a cell-surface glycoprotein expressed on prostate cancer cells and the neovascular endothelium of multiple carcinomas. We show that primary T cells harvested from five of five patients with different stages of prostate cancer and transduced with the Pz-1 receptor readily lyse prostate cancer cells. Having established a culture system using fibroblasts that express PSMA, we next show that T cells expressing the Pz-1 receptor release cytokines in response to cell-bound PSMA. Furthermore, we show that the cytokine release is greatly augmented by B7.1-mediated costimulation. Thus, our findings support the feasibility of adoptive cell therapy by using genetically engineered T cells in prostate cancer patients and suggest that both CD4+ and CD8+ T lymphocyte functions can be synergistically targeted against tumor cells

Quantifying the impact of key factors on the carbon mitigation potential of managed temperate forests

Background: Forests mitigate climate change by reducing atmospheric CO2-concentrations through the carbon sink in the forest and in wood products, and substitution effects when wood products replace carbon-intensive materials and fuels. Quantifying the carbon mitigation potential of forests is highly challenging due to the influence of multiple important factors such as forest age and type, climate change and associated natural disturbances, harvest intensities, wood usage patterns, salvage logging practices, and the carbon-intensity of substituted products. Here, we developed a framework to quantify the impact of these factors through factorial simulation experiments with an ecosystem model at the example of central European (Bavarian) forests. Results: Our simulations showed higher mitigation potentials of young forests compared to mature forests, and similar ones in broad-leaved and needle-leaved forests. Long-lived wood products significantly contributed to mitigation, particularly in needle-leaved forests due to their wood product portfolio, and increased material usage of wood showed considerable climate benefits. Consequently, the ongoing conversion of needle-leaved to more broad-leaved forests should be accompanied by the promotion of long-lived products from broad-leaved species to maintain the product sink. Climate change (especially increasing disturbances) and decarbonization were among the most critical factors influencing mitigation potentials and introduced substantial uncertainty. Nevertheless, until 2050 this uncertainty was narrow enough to derive robust findings. For instance, reducing harvest intensities enhanced the carbon sink in our simulations, but diminished substitution effects, leading to a decreased total mitigation potential until 2050. However, when considering longer time horizons (i.e. until 2100), substitution effects became low enough in our simulations due to expected decarbonization such that decreasing harvests often seemed the more favorable solution. Conclusion: Our results underscore the need to tailor mitigation strategies to the specific conditions of different forest sites. Furthermore, considering substitution effects, and thoroughly assessing the amount of avoided emissions by using wood products, is critical to determine mitigation potentials. While short-term recommendations are possible, we suggest risk diversification and methodologies like robust optimization to address increasing uncertainties from climate change and decarbonization paces past 2050. Finally, curbing emissions reduces the threat of climate change on forests, safeguarding their carbon sink and ecosystem services

Revealing the Formation of the Milky Way Nuclear Star Cluster via Chemo-Dynamical Modeling

The Milky Way nuclear star cluster (MW NSC) has been used as a template to understand the origin and evolution of galactic nuclei and the interaction of nuclear star clusters with supermassive black holes. It is the only nuclear star cluster with a supermassive black hole where we can resolve individual stars to measure their kinematics and metal abundance to reconstruct its formation history. Here, we present results of the first chemo-dynamical model of the inner 1 pc of the MW NSC using metallicity and radial velocity data from the KMOS spectrograph on the Very Large Telescope. We find evidence for two kinematically and chemically distinct components in this region. The majority of the stars belong to a previously known super-solar metallicity component with a rotation axis perpendicular to the Galactic plane. However, we identify a new kinematically distinct sub-solar metallicity component which contains about 7\% of the stars and appears to be rotating faster than the main component with a rotation axis that may be misaligned. This second component may be evidence for an infalling star cluster or remnants of a dwarf galaxy, merging with the MW NSC. These measurements show that the combination of chemical abundances with kinematics is a promising method to directly study the MW NSC's origin and evolution.Comment: 6 pages, 5 figures, accepted to ApJ Letter