Estudos de propagação em Smilax fluminensis Steud. (Smilacaceae)

Smilax L. species, popularly known in Brazil as salsaparrilha, have been used in folk medicine as tonic against rheumatism and as anti-syphilitic since the sixteenth century; however, until today, their roots have been explored in an extractive way. The aim of this study was to analyze the propagation of Smilax fluminensis aiming to meet the demand and to propose procedures for its sustainable management. It was analyzed the percentage of seed germination, rooting of aerial and subterranean stem cuttings and plant resprouting capacity after removal of the shoots. The monitoring lasted one year. Seeds were subjected to temperatures: 25°C, 30°C, 20-30°C and 20-35°C under a daily photoperiod of eight hours and in the absence of light. For rooting the stem cuttings, aerial and subterranean stems about 20cm long with two nodal regions were subjected to treatment with distilled water (control) and indolbutyric acid (IBA) at 100ppm. The best germination percentages obtained for S. fluminensis were 80% at 20-30°C under light and 85% at 30°C in the dark. Only subterranean stem cuttings showed significant difference concerning fresh and dry matter of roots with higher values in treatments with hormone compared to the control.As espécies do gênero Smilax L., conhecidas popularmente como salsaparrilha, são empregadas na medicina popular como fortificante contra o reumatismo e antissifilítico desde o século XVI, porém até hoje as suas raízes são exploradas de maneira extrativista. O objetivo deste estudo foi analisar a propagação de Smilax fluminensis visando a auxiliar no atendimento da demanda e apontar propostas para o seu manejo sustentável. Foram analisados a porcentagem de germinação de sementes, o enraizamento de estacas de ramos aéreos e subterrâneos e o acompanhamento ao longo de um ano da capacidade de rebrotamento das plantas após a retirada dos ramos aéreos. As sementes foram submetidas às temperaturas: 25, 30, 20-30 e 20-35°C, sob fotoperíodo diário de oito horas e na ausência de luz. Para o enraizamento de estacas, foram utilizados ramos aéreos e subterrâneos com aproximadamente 20cm com duas regiões nodais submetidas ao tratamento com água destilada (controle) e com ácido indolbutírico (AIB) a 100ppm. As melhores porcentagens de germinação obtidas para S. fluminensis, 80% em 20-30°C no claro e 85% a 30°C no escuro. Apenas as estacas de ramos subterrâneos enraizaram, houve diferença significativa em relação à massa fresca e seca de raízes com valores maiores nos tratamentos com hormônio em relação ao controle

Germination and seedling morphology of four South American Smilax (Smilacaceae)

Species of Smilax,, also known as greenbrier, are widely distributed in Brazil and their commercial trades are carried out by the extractivism of native species. We the aim to provide information about the germination and development of seedlings in four Smilax species, different experiments were developed under controlled conditions. We evaluated two germination treatments: temperature (30 degrees C and 20-30 degrees C) and light (presence/absence), and for few cases the tetrazolium treatment was applied. A different treatment response was observed among the studied species. Light had a significant influence in S. brasiliensis, with the highest germination rates at 20-30 C in dark conditions. S. campestris showed significant differences among temperature treatments, but not to light; while S. cissoides showed high germination rates (66-78%), independently of treatment. However, S. polyantha had low germination rates (19-24%). After one year, the expanded leaves showed different characteristics among the studied species. Leaves of S. brasiliensis were ovate, coriaceous, three main veins and prickle-like structures only on the midrib on abaxial face. S. campestris leaves were oblong, coriaceous and prickle-like structures were located at the leaf midrib and margin. S. cissoides had ovate-elliptic, membranaceous leaves, with three main veins with prickle-like structures on the abaxial face. S. polyantha leaves showed ovate-elliptic. coriaceous leaves, with three main veins, translucent secondary veins and no prickle-like structures. A seedling identification key was elaborated based on morphological characteristics. Rev. Biol. Trop. 60 (1): 495-504. Epub 2012 March 01.FAPESP (Sao Paulo Council for Research) BIOTAFAPESP (Sao Paulo Council for Research) - BIOTA [05/54984-5, 05/58964-9]CNPqCNP

Morph-anatomy, chemical profile and propagation of Smilax fluminensis Steud. (Smilacaceae)

As espécies do gênero Smilax L., conhecidas popularmente como salsaparrilha, são empregadas na medicina popular desde o século XVI, porém essas plantas ainda são exploradas de maneira extrativista. Com o aumento da comercialização de plantas medicinais cresce a necessidade de trabalhos que certifiquem a qualidade da matéria prima. A caracterização anatômica e o perfil químico certamente fornecem uma base mais segura nessa certificação. Visando auxiliar no atendimento da demanda e apontar propostas do manejo sustentável em áreas de ocorrência natural de Smilax fluminensis Steud. os objetivos do presente estudo foram: a) analisar a morfoanatomia dos órgãos vegetativos e indicar as características de valor diagnóstico para essa espécie; b) analisar o perfil químico de S. fluminensis através da Cromatografia Líquida de Alta Eficiência acoplada com Detector de Arranjo de Diodos utilizando-se extratos metanólicos (EMeOH) de raízes, rizóforos e ramos aéreos; c) realizar estudos de germinação de sementes e de propagação vegetativa por estacas de ramos aéreos e subterrâneos e, d) acompanhar ao longo de um ano a capacidade de rebrotamento das plantas no campo. As análises ao microscópio de luz foram realizadas utilizando-se as técnicas usuais para o preparo de lâminas semipermamentes e permanentes. Para as análises no microscópio eletrônico de varredura, amostras de folhas foram fixadas em Karnovsky, desidratadas em série etílica e submetidas ao método do ponto crítico de CO2, montadas sobre suportes de alumínio e metalizadas. As sementes foram submetidas a diferentes temperaturas, sob fotoperíodo diário de oito horas e na ausência de luz. Para o enraizamento de estacas, foram utilizados ramos aéreos e subterrâneos com aproximadamente 20 cm, com duas regiões nodais submetidas ao tratamento com ácido indolbutírico a 100 ppm ou apenas em água destilada. Dentre os caracteres anatômicos que permitiram a delimitação da espécie destacam-se: estômatos anisocíticos e paracíticos presentes na epiderme da face abaxial; cera epicuticular na forma de grânulos globosos; mesofilo homogêneo com ampla câmara subestomática; presença da bainha amilífera nos primeiros entrenós do caule aéreo; presença de endoderme com reforço em U; ausência de meristema de espessamento no rizóforo adulto; presença de exoderme com estrias de Caspary nas raízes brancas e ausência de córtex interno nas raízes marrons. Em relação ao perfil químico, os extratos obtidos apresentaram picos correspondentes às substâncias: ácido clorogênico, ácido cafeico, rutina, ácido p-cumárico, ácido ferúlico e ácido trans-cinâmico. As melhores porcentagens de germinação foram 80% em 20-30º C no claro e 85% a 30ºC no escuro. Apenas as estacas de ramos subterrâneos enraizaram. Ao final de um ano de acompanhamento das plantas no campo, todas apresentaram em média 4.05 novos brotamentos. As espécies cujas partes utilizadas para o preparo dos medicamentos são as raízes correm maior risco de extinção como é o caso de S. fluminensis. Portanto, a capacidade de propagação por sementes, por estacas e de regeneração de ramos aéreos após a remoção de parte das estruturas subterrâneas aliada ao perfil químico confirma o seu potencial para a exploração econômica de maneira sustentável, sendo uma alternativa para reduzir o extrativismo predatório dessa espécie nativa.The Smilax L. species, popularly known as salsaparrilha, have been used in folk medicine since the sixteenth century; however, these plants have still been handled in extractive way. With the increasing commercialization of medicinal plants, there is need to study to certify the raw material quality. Anatomical analyses and chemical profile characterization certainly provides a more secure basis to this certification. Aiming to meet the demand and to point proposals for sustainable management of natural occurrence of Smilax fluminensis Steud., the objectives of this study were: a) to analyze the morph-anatomy of vegetative organs and indicate the features of diagnostic value for this species, b) to examine the chemical profile of S. fluminensis by High Performance Liquid Chromatography Coupled with Diode Array Detector using methanol extracts (EMeOH) of roots, rhizophore and stems c) to conduct studies of seed germination and vegetative propagation on cuttings of aerial and subterranean stems and d) to monitor, over a year, the resprouting ability of plants in the field. We carried out the analyses under a light microscopy using the usual techniques for preparing semi-permanent and permanent slides. To perform the analyses under a scanning electron microscopy, leaf samples were fixed in Karnovsky, dehydrated in ethanol series and subjected to critical point method of CO2, mounted on aluminum supports and coated with gold. Seeds were exposed to different temperatures under a daily photoperiod of eight hours and in the absence of light. To analyze the sprouting, aerial and subterranean stems were used with about 20 cm of length, with two nodal regions subjected to treatment with IBA at 100 ppm or in distilled water. Among the anatomical features that enable delimitation of the species are: anisocytic and paracytic stomata in the epidermis of the abaxial surface, epicuticular wax in the form of granules globules, homogeneous mesophyll with large substomatal chambers, presence of starch sheath in the first internodes of the aerial stem, the presence of endoderm-reinforced U and the absence of primary thickening meristem in the adult rhizophore, exodermis with Casparian strips in roots, and the absence of inner cortex in brown roots. Regarding the chemical profile, extracts showed peaks corresponding to the following substances: chlorogenic acid, caffeic acid, rutin, p-coumaric acid, ferulic acid and trans-Cinnamic acid. The best germination was 80% at 20-30ºC under light and 85% at 30ºC in the dark. Only roots sprouted. After one year of monitoring the plants in the field, all had an average of 4.05 new aerial shoots. The species whose roots are used for the preparation of medicines face a greater risk of extinction as is the case of S. fluminensis. Therefore, the ability to spread by seeds, cuttings and shoots regeneration after removal of aerial stems combined with the chemical profile confirms its potential for economic use in a sustainable manner, and an alternative to reduce the predatory extraction of native species

Morph-anatomy, chemical profile and propagation of Smilax fluminensis Steud. (Smilacaceae)

As espécies do gênero Smilax L., conhecidas popularmente como salsaparrilha, são empregadas na medicina popular desde o século XVI, porém essas plantas ainda são exploradas de maneira extrativista. Com o aumento da comercialização de plantas medicinais cresce a necessidade de trabalhos que certifiquem a qualidade da matéria prima. A caracterização anatômica e o perfil químico certamente fornecem uma base mais segura nessa certificação. Visando auxiliar no atendimento da demanda e apontar propostas do manejo sustentável em áreas de ocorrência natural de Smilax fluminensis Steud. os objetivos do presente estudo foram: a) analisar a morfoanatomia dos órgãos vegetativos e indicar as características de valor diagnóstico para essa espécie; b) analisar o perfil químico de S. fluminensis através da Cromatografia Líquida de Alta Eficiência acoplada com Detector de Arranjo de Diodos utilizando-se extratos metanólicos (EMeOH) de raízes, rizóforos e ramos aéreos; c) realizar estudos de germinação de sementes e de propagação vegetativa por estacas de ramos aéreos e subterrâneos e, d) acompanhar ao longo de um ano a capacidade de rebrotamento das plantas no campo. As análises ao microscópio de luz foram realizadas utilizando-se as técnicas usuais para o preparo de lâminas semipermamentes e permanentes. Para as análises no microscópio eletrônico de varredura, amostras de folhas foram fixadas em Karnovsky, desidratadas em série etílica e submetidas ao método do ponto crítico de CO2, montadas sobre suportes de alumínio e metalizadas. As sementes foram submetidas a diferentes temperaturas, sob fotoperíodo diário de oito horas e na ausência de luz. Para o enraizamento de estacas, foram utilizados ramos aéreos e subterrâneos com aproximadamente 20 cm, com duas regiões nodais submetidas ao tratamento com ácido indolbutírico a 100 ppm ou apenas em água destilada. Dentre os caracteres anatômicos que permitiram a delimitação da espécie destacam-se: estômatos anisocíticos e paracíticos presentes na epiderme da face abaxial; cera epicuticular na forma de grânulos globosos; mesofilo homogêneo com ampla câmara subestomática; presença da bainha amilífera nos primeiros entrenós do caule aéreo; presença de endoderme com reforço em U; ausência de meristema de espessamento no rizóforo adulto; presença de exoderme com estrias de Caspary nas raízes brancas e ausência de córtex interno nas raízes marrons. Em relação ao perfil químico, os extratos obtidos apresentaram picos correspondentes às substâncias: ácido clorogênico, ácido cafeico, rutina, ácido p-cumárico, ácido ferúlico e ácido trans-cinâmico. As melhores porcentagens de germinação foram 80% em 20-30º C no claro e 85% a 30ºC no escuro. Apenas as estacas de ramos subterrâneos enraizaram. Ao final de um ano de acompanhamento das plantas no campo, todas apresentaram em média 4.05 novos brotamentos. As espécies cujas partes utilizadas para o preparo dos medicamentos são as raízes correm maior risco de extinção como é o caso de S. fluminensis. Portanto, a capacidade de propagação por sementes, por estacas e de regeneração de ramos aéreos após a remoção de parte das estruturas subterrâneas aliada ao perfil químico confirma o seu potencial para a exploração econômica de maneira sustentável, sendo uma alternativa para reduzir o extrativismo predatório dessa espécie nativa.The Smilax L. species, popularly known as salsaparrilha, have been used in folk medicine since the sixteenth century; however, these plants have still been handled in extractive way. With the increasing commercialization of medicinal plants, there is need to study to certify the raw material quality. Anatomical analyses and chemical profile characterization certainly provides a more secure basis to this certification. Aiming to meet the demand and to point proposals for sustainable management of natural occurrence of Smilax fluminensis Steud., the objectives of this study were: a) to analyze the morph-anatomy of vegetative organs and indicate the features of diagnostic value for this species, b) to examine the chemical profile of S. fluminensis by High Performance Liquid Chromatography Coupled with Diode Array Detector using methanol extracts (EMeOH) of roots, rhizophore and stems c) to conduct studies of seed germination and vegetative propagation on cuttings of aerial and subterranean stems and d) to monitor, over a year, the resprouting ability of plants in the field. We carried out the analyses under a light microscopy using the usual techniques for preparing semi-permanent and permanent slides. To perform the analyses under a scanning electron microscopy, leaf samples were fixed in Karnovsky, dehydrated in ethanol series and subjected to critical point method of CO2, mounted on aluminum supports and coated with gold. Seeds were exposed to different temperatures under a daily photoperiod of eight hours and in the absence of light. To analyze the sprouting, aerial and subterranean stems were used with about 20 cm of length, with two nodal regions subjected to treatment with IBA at 100 ppm or in distilled water. Among the anatomical features that enable delimitation of the species are: anisocytic and paracytic stomata in the epidermis of the abaxial surface, epicuticular wax in the form of granules globules, homogeneous mesophyll with large substomatal chambers, presence of starch sheath in the first internodes of the aerial stem, the presence of endoderm-reinforced U and the absence of primary thickening meristem in the adult rhizophore, exodermis with Casparian strips in roots, and the absence of inner cortex in brown roots. Regarding the chemical profile, extracts showed peaks corresponding to the following substances: chlorogenic acid, caffeic acid, rutin, p-coumaric acid, ferulic acid and trans-Cinnamic acid. The best germination was 80% at 20-30ºC under light and 85% at 30ºC in the dark. Only roots sprouted. After one year of monitoring the plants in the field, all had an average of 4.05 new aerial shoots. The species whose roots are used for the preparation of medicines face a greater risk of extinction as is the case of S. fluminensis. Therefore, the ability to spread by seeds, cuttings and shoots regeneration after removal of aerial stems combined with the chemical profile confirms its potential for economic use in a sustainable manner, and an alternative to reduce the predatory extraction of native species

New approaches to underground systems in Brazilian Smilax species (Smilacaceae)

Scientific studies show that the watery extract of the thickened underground stem and its adventitious roots of the genus Smilax can act as a therapeutic agent in immunoinflammatory disorders, such as rheumatic arthritis. Brazilians have used this genus of plants in folk medicine, however it is very hard to identify these species, since the morphology of the underground systems is very similar in this group. For better identification of those systems, we studied six species of Smilax L. (S. brasiliensis, S. campestris, S. cissoides, S. goyazana, S. oblongifolia and S. rufescens), collected in different regions of Brazil with different physiognomies and soil characteristics. The main purpose is to describe the morpho-anatomy of the underground systems and to analyze if their structure depends on environmental conditions. The underground stem (rhizophore) is of brown color and it is knotty, massive, slender (S. rufescens) or tuberous (S. brasiliensis, S. campestris, S. cissoides, S. goyazana and S. oblongifolia). The tuberization is a result of primary thickened meristem (PTM) activity. The color and thickness of the adventitious roots change during development because the epidermis and outer cortex are disposed of, so the inner cortex becomes the new covering tissue with lignified and dark color cells. There are differences in starch grain shapes in mature roots. The chemical attributes of the soil are very similar in all studied environments and, even when soil characteristics varied, all the species' underground system was distributed close to the soil surface (10 to 15 cm deep). The species exhibited clonal growth hence their underground system functions as storage structures and the axillary buds can sprout into new stems. Only Smilax rufescens, collected in sandy soil of Restinga, has vegetative dispersal due to the runners1372-3220235CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçãosem informação05/54984-5; 05/58964-

New approaches to underground systems in Brazilian Smilax species (Smilacaceae)

MARTINS, A. R. (Institute of Biology, State University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil), N. PUT, (Division of Biology and Education, University of Vechta, 49377 Vechta, Germany), A. N. SOARES, A.B BOMB, and B. APPEZZATO DA GLORIA (Biological Science Department, Escola Superior de Agricultura `Luiz de Queiroz`, University of Sao Paulo, 13418-900, Piracicaba, SP, Brazil). J. Torrey Bot. Soc. 137: 220-235. 2010.-New approaches to underground systems in Brazilian Smilax species (Smilacaceae). Scientific studies show that the watery extract of the thickened underground stem and its adventitious roots of the genus Smilax can act as a therapeutic agent in immunoinflammatory disorders, such as rheumatic arthritis. Brazilians have used this genus of plants in folk medicine, however it is very hard to identify these species, since the morphology of the underground systems is very similar in this group. For better identification of those systems, we studied six species of Smilax L. (S. brasiliensis, S. campestris, S. cissoides, S. goyazana, S. oblongifolia and S. rufescens), collected in different regions of Brazil with different physiognomies and soil characteristics. The main purpose is to describe the morpho-anatomy of the underground systems and to analyze if their structure depends on environmental conditions. The underground stem (rhizophore) is of brown color and it is knotty, massive, slender (S. rufescens) or tuberous (S. brasiliensis, S. campestris, S. cissoides, S. goyazana and S. oblongifolia). The tuberization is a result of primary thickened meristem (PTM) activity. The color and thickness of the adventitious roots change during development because the epidermis and outer cortex are disposed of, so the inner cortex becomes the new covering tissue with lignified and dark color cells. There are differences in starch grain shapes in mature roots. The chemical attributes of the soil are very similar in all studied environments and, even when soil characteristics varied, all the species` underground system was distributed close to the soil surface (10 to 15 cm deep). The species exhibited clonal growth hence their underground system functions as storage structures and the axillary buds can sprout into new stems. Only Smilax rufescens, collected in sandy soil of Restinga, has vegetative dispersal due to the runners.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/54984-5]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/58964-9]CAPESCNP

Germination And Seedling Morphology Of Four South American Smilax (smilacaceae).

Species of Smilax, also known as greenbrier, are widely distributed in Brazil and their commercial trades are carried out by the extractivism of native species. We the aim to provide information about the germination and development of seedlings in four Smilax species, different experiments were developed under controlled conditions. We evaluated two germination treatments: temperature (30 degrees C and 20-30 degrees C) and light (presence/ absence), and for few cases the tetrazolium treatment was applied. A different treatment response was observed among the studied species. Light had a significant influence in S. brasiliensis, with the highest germination rates at 20-30 degrees C in dark conditions. S. campestris showed significant differences among temperature treatments, but not to light; while S. cissoides showed high germination rates (66-78%), independently of treatment. However, S. polyantha had low germination rates (19-24%). After one year, the expanded leaves showed different characteristics among the studied species. Leaves of S. brasiliensis were ovate, coriaceous, three main veins and prickle-like structures only on the midrib on abaxial face. S. campestris leaves were oblong, coriaceous and prickle-like structures were located at the leaf midrib and margin. S. cissoides had ovate-elliptic, membranaceous leaves, with three main veins with prickle-like structures on the abaxial face. S. polyantha leaves showed ovate-elliptic, coriaceous leaves, with three main veins, translucent secondary veins and no prickle-like structures. A seedling identification key was elaborated based on morphological characteristics.60495-50