947 research outputs found
Inconsistent genetic structure among members of a multitrophic system: did bruchid parasitoids (Horismenus spp.) escape the effects of bean domestication?
Anthropogenic range expansion and cultural practices have modified the distribution, abundance and genetic diversity of domesticated organisms, thereby altering multitrophic assemblages through space and time. The putative Mesoamerican domestication centre of the common bean, Phaseolus vulgaris L., in Mexico allows investigating the effects of plant domestication on the genetic structure of members of a multitrophic system. The aim of this study was to compare the evolutionary history of Horismenus parasitoids (Hymenoptera: Eulophidae) to those of their bruchid beetle hosts (Coleoptera: Bruchidae) and their domesticated host plant (P. vulgaris), in the context of traditional agriculture in Mexico. We analyzed the population genetic structure of four Horismenus species in Mexico using mitochondrial COI haplotype data. The two most abundant parasitoid species were Horismenus depressus and Horismenus missouriensis. Horismenus missouriensis were infected by Wolbachia endosymbionts and had little to no population differentiation (F ST=0.06). We suspect the mitochondrial history of H. missouriensis to be blurred by Wolbachia, because differentiation among infected vs. non-infected individuals exists (F ST=0.11). Populations of H. depressus were found to be highly differentiated (F ST=0.34), but the genetic structuring could not be explained by tested spatial components. We then compared the genetic structure observed in this parasitoid species to previously published studies on bruchid beetles and their host plants. Despite extensive human-mediated migration and likely population homogenization of its two Acanthoscelides bruchid beetle hosts, H. depressus populations are structured like its host plant, by a recent dispersal from a diverse ancestral gene pool. Distinct evolutionary dynamics may explain inconsistent patterns among trophic levels. Parasitoids likely migrate from wild bean populations and are poorly adapted to bean storage conditions similar to their bruchid beetle hosts. Integrating several trophic levels to the study of evolutionary history has proven to be fruitful in detecting different ecological responses to human-mediated disturbances and host parasite interaction
ENTH/ANTH proteins and clathrin-mediated membrane budding
The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. Structural analyses and ligand-binding studies have shown that a set of proteins previously designated as harboring an ENTH domain in fact contain a highly similar, yet unique module referred to as an AP180 N-terminal homology (ANTH) domain. ENTH and ANTH (E/ANTH) domains bind both inositol phospholipids and proteins and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. E/ANTH-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the trans-Golgi network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding
Endophilin regulates JNK activation through its interaction with the germinal center kinase-like kinase
The endophilin family of proteins function in clathrin-mediated endocytosis. Here, we have identified and cloned the rat germinal center kinase-like kinase (rGLK), a member of the GCK (germinal center kinase) family of e-Jun N-terminal kinase (JNK) activating enzymes, as a novel endophilin I-binding partner. The interaction occurs both in vitro and in cells and is mediated by the Src homology 3 domain of endophilin I and a region of rGLK containing the endophilin consensus-binding sequence PPRPPPPR. Overlay analysis of rat brain extracts demonstrates that endophilin I is a major Src homology 3 domain-binding partner for rGLK. Overexpression of full-length endophilin I activates rGLK-mediated JNK activation, whereas N- and C-terminal fragments of endophilin I block JNK activation. Thus, endophilin I appears to have a novel function in JNK activation
Proximity DC squids in the long junction limit
We report the design and measurement of
Superconducting/normal/superconducting (SNS) proximity DC squids in the long
junction limit, i.e. superconducting loops interrupted by two normal metal
wires roughly a micrometer long. Thanks to the clean interface between the
metals, at low temperature a large supercurrent flows through the device. The
dc squid-like geometry leads to an almost complete periodic modulation of the
critical current through the device by a magnetic flux, with a flux periodicity
of a flux quantum h/2e through the SNS loop. In addition, we examine the entire
field dependence, notably the low and high field dependence of the maximum
switching current. In contrast with the well-known Fraunhoffer-type
oscillations typical of short wide junctions, we find a monotonous gaussian
extinction of the critical current at high field. As shown in [15], this
monotonous dependence is typical of long and narrow diffusive junctions. We
also find in some cases a puzzling reentrance at low field. In contrast, the
temperature dependence of the critical current is well described by the
proximity effect theory, as found by Dubos {\it et al.} [16] on SNS wires in
the long junction limit. The switching current distributions and hysteretic IV
curves also suggest interesting dynamics of long SNS junctions with an
important role played by the diffusion time across the junction.Comment: 12 pages, 16 figure
Direct measurement of the phase coherence length in a GaAs/GaAlAs square network
The low temperature magnetoconductance of a large array of quantum
coherentloops exhibits Altshuler-Aronov-Spivak oscillations which
periodicitycorresponds to 1/2 flux quantum per loop.We show that the
measurement of the harmonics content in a square networkprovides an accurate
way to determine the electron phase coherence length in units of the
lattice length without any adjustableparameters.We use this method to determine
in a network realised from a 2Delectron gas (2DEG) in a GaAS/GaAlAs
heterojunction. The temperaturedependence follows a power law from
1.3 K to 25 mK with nosaturation, as expected for 1D diffusive electronic
motion andelectron-electron scattering as the main decoherence mechanism.Comment: Additional experimental data in version
Linking epigenetics and biological conservation: Towards a conservation epigenetics perspective
International audience1. Biodiversity conservation is a global issue where the challenge is to integrate all levels of biodiversity to ensure the long-term evolutionary potential and resilience of biological systems. Genetic approaches have largely contributed to conservation biology by defining "conservation entities" accounting for their evolutionary history and adaptive potential, the so-called evolutionary significant units (ESUs). Yet, these approaches only loosely integrate the short-term ecological history of organisms. 2. Here, we argue that epigenetic variation, and more particularly DNA methylation, represents a molecular component of biodiversity that directly links the genome to the environment. As such, it provides the required information on the ecological background of organisms for an integrative field of conservation biology. 3. We synthesize knowledge about the importance of epigenetic mechanisms in (a) orchestrating fundamental development alternatives in organisms, (b) enabling individuals to respond in real-time to selection pressures and (c) improving ecosystem stability and functioning. 4. Using practical examples in conservation biology, we illustrate the relevance of DNA methylation (a) as biomarkers of past and present environmental stress events as well as biomarkers of physiological conditions of individuals; (b) for documenting the ecological structuring/clustering of wild populations and hence for better integrating ecology into ESUs; (c) for improving conservation transloca-tions; and (d) for studying landscape functional connectivity. 5. We conclude that an epigenetic conservation perspective will provide environmental managers the possibility to refine ESUs, to set conservation plans taking into account the capacity of organisms to rapidly cope with environmental changes, and hence to improve the conservation of wild populations. K E Y W O R D S conservation, DNA methylation, ecological timescales, epigenetic, evolutionary significant unit
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