New Mechanisms of Action of Natural Antioxidants in Health and Disease

Natural antioxidants have been proposed to have beneficial effects on health and on different disease states, such as neurodegenerative and cardiovascular diseases, diabetes and cancer. The use of natural plant antioxidant products to handle different diseases has very ancient roots; well before the development of modern medicine with synthetic drugs and antioxidants. A lot of the biological activities of natural antioxidants have been ascribed to their ability to scavenge reactive oxygen species (ROS) that counteract oxidative stress. In the last years, a multitude of studies have suggested that their classical hydrogen-donating antioxidant activity is unlikely to be the sole explanation for their effects. First of all, natural antioxidants are subjected to an extensive metabolism in vivo that modifies their redox potentials. Moreover, the concentration of natural antioxidants and their metabolites in vivo are lower than that usually utilized in vitro. Accumulating evidence suggests that the cellular effects of natural antioxidants may also be mediated by their interactions with specific proteins central to intracellular signaling cascades, their modulation of the expression and activity of key proteins, their influencing of epigenetic mechanisms or their modulation of the gut microbiota. This special issue, concerning new mechanisms in the action of natural antioxidants in health and disease, contains nine contributions, seven research articles and two reviews, and details recent advances on this topic

Neuroprotection by Drugs, Nutraceuticals and Physical Activity

Acute and chronic neural injuries, including stroke, brain trauma and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Huntington's disease (HD), Parkinson's disease (PD), and Alzheimer's disease (AD) are associated with high morbidity and mortality rates [...]

New mechanisms of action of natural antioxidants in health and disease II

Epidemiological studies have demonstrated an association between natural antioxidants and human health [...]

Combined treatment with three natural antioxidants enhances neuroprotection in a SH-SY5Y 3D culture model

Currently, the majority of cell-based studies on neurodegeneration are carried out on two-dimensional cultured cells that do not represent the cells residing in the complex microenvironment of the brain. Recent evidence has suggested that three-dimensional (3D) in vitro microenvironments may better model key features of brain tissues in order to study molecular mechanisms at the base of neurodegeneration. So far, no drugs have been discovered to prevent or halt the progression of neurodegenerative disorders. New therapeutic interventions can come from phytochemicals that have a broad spectrum of biological activities. On this basis, we evaluated the neuroprotective effect of three phytochemicals (sulforaphane, epigallocatechin gallate, and plumbagin) alone or in combination, focusing on their ability to counteract oxidative stress. The combined treatment was found to be more effective than the single treatments. In particular, the combined treatment increased cell viability and reduced glutathione (GSH) levels, upregulated antioxidant enzymes and insulin-degrading enzymes, and downregulated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 and 2 in respect to peroxide-treated cells. Our data suggest that a combination of different phytochemicals could be more effective than a single compound in counteracting neurodegeneration, probably thanks to a pleiotropic mechanism of action

The WFCAM Multi-wavelength Variable Star Catalog

Stellar variability in the near-infrared (NIR) remains largely unexplored. The exploitation of public science archives with data-mining methods offers a perspective for the time-domain exploration of the NIR sky. We perform a comprehensive search for stellar variability using the optical-NIR multi-band photometric data in the public Calibration Database of the WFCAM Science Archive (WSA), with the aim of contributing to the general census of variable stars, and to extend the current scarce inventory of accurate NIR light curves for a number of variable star classes. We introduce new variability indices designed for multi-band data with correlated sampling, and apply them for pre-selecting variable star candidates, i.e., light curves that are dominated by correlated variations, from noise-dominated ones. Pre-selection criteria are established by robust numerical tests for evaluating the response of variability indices to colored noise characteristic to the data. We find 275 periodic variable stars and an additional 44 objects with suspected variability with uncertain periods or apparently aperiodic variation. Only 44 of these objects had been previously known, including 11 RR~Lyrae stars in the outskirts of the globular cluster M3 (NGC~5272). We provide a preliminary classification of the new variable stars that have well-measured light curves, but the variability types of a large number of objects remain ambiguous. We classify most of the new variables as contact binary stars, but we also find several pulsating stars, among which 34 are probably new field RR~Lyrae and 3 are likely Cepheids. We also identify 32 highly reddened variable objects close to previously known dark nebulae, suggesting that these are embedded young stellar objects. We publish our results and all light-curve data as the WFCAM Variable Star Catalog.Comment: 21 pages, 11 figure

The use of dietary supplements among soccer referees: How much do they know?

Referees are essential for the correct execution of each soccer match and national soccer associations support them with physical training guidelines in order to improve agility, aerobic and sprint capacity, to reduce the risk of injury and to meet the minimum required performance levels. Despite the importance given to performance skills no specific nutritional guideline is provided to support the performance required. This study was aimed to investigate soccer field and assistant referees knowledge and consumption of dietary supplements. Seventy-one European referees participated to this survey by filling a short and quick anonymous questionnaire designed to collect information about their refereeing category, educational level, frequency of supplement consumption, type of supplement consumed. Moreover, a sub-group of 20 participants wore a training watch to record energy expenditure, distance, and heart rate during training sessions and matches. Our data show that referees frequently consume supplements especially those refereeing in lower categories, moreover they often follow a friend\u2019s suggestion when deciding to take a supplement instead of trusting a professional prescription. According to our survey, national and international soccer federations should care more about their referee nutritional education

Antioxidant and Anti-Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Spent coffee grounds (SCGs), waste products of coffee beverage production, are rich in organic compounds such as phenols. Different studies have demonstrated phenol beneficial effects in counteracting neurodegenerative diseases. These diseases are associated with oxidative stress and neuroinflammation, which initiates the degeneration of neurons by overactivating microglia. Unfortunately, to date, there are no pharmacological therapies to treat these pathologies. The aim of this study was to evaluate the phenolic content of 4 different SCG extracts and their ability to counteract oxidative stress and neuroinflammation. Caffeine and 5-O-caffeoylquinic acid were the most abundant compounds in all extracts, followed by 3-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid. The four extracts demonstrated a different ability to counteract oxidative stress and neuroinflammation in vitro. In particular, the methanol extract was the most effective in protecting neuron-like SH-SY5Y cells against H2O2-induced oxidative stress by upregulating endogenous antioxidant enzymes such as thioredoxin reductase, heme oxygenase 1, NADPH quinone oxidoreductase, and glutathione reductase. The water extract was the most effective in counteracting lipopolysaccharide-induced neuroinflammation in microglial BV-2 cells by strongly reducing the expression of proinflammatory mediators through the modulation of the TLR4/NF-kappa B pathway. On these bases, SCG extracts could represent valuable nutraceutical sources for the treatment of neurodegeneratio

Role of Mesenchymal Stem Cells in Counteracting Oxidative Stress\u2014Related Neurodegeneration

Neurodegenerative diseases include a variety of pathologies such as Alzheimer\u2019s disease, Parkinson\u2019s disease, Huntington\u2019s disease, amyotrophic lateral sclerosis, and so forth, which share many common characteristics such as oxidative stress, glycation, abnormal protein deposition, inflammation, and progressive neuronal loss. The last century has witnessed significant research to identify mechanisms and risk factors contributing to the complex etiopathogenesis of neurodegenerative diseases, such as genetic, vascular/metabolic, and lifestyle-related factors, which often co-occur and interact with each other. Apart from several environmental or genetic factors, in recent years, much evidence hints that impairment in redox homeostasis is a common mechanism in different neurological diseases. However, from a pharmacological perspective, oxidative stress is a difficult target, and antioxidants, the only strategy used so far, have been ineffective or even provoked side effects. In this review, we report an analysis of the recent literature on the role of oxidative stress in Alzheimer\u2019s and Parkinson\u2019s diseases as well as in amyotrophic lateral sclerosis, retinal ganglion cells, and ataxia. Moreover, the contribution of stem cells has been widely explored, looking at their potential in neuronal differentiation and reporting findings on their application in fighting oxidative stress in different neurodegenerative diseases. In particular, the exposure to mesenchymal stem cells or their secretome can be considered as a promising therapeutic strategy to enhance antioxidant capacity and neurotrophin expression while inhibiting pro-inflammatory cytokine secretion, which are common aspects of neurodegenerative pathologies. Further studies are needed to identify a tailored approach for each neurodegenerative disease in order to design more effective stem cell therapeutic strategies to prevent a broad range of neurodegenerative disorder