Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells

Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.Fil: Wu, Ling. University of California at Los Angeles; Estados UnidosFil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados UnidosFil: Kyupelyan, Levon. University of California at Los Angeles; Estados UnidosFil: Latour, Brooke. University of California at Los Angeles; Estados UnidosFil: Gonzalez, Stephanie. University of California at Los Angeles; Estados UnidosFil: Shah, Saumya. University of California at Los Angeles; Estados UnidosFil: Galic, Zoran. University of California at Los Angeles; Estados UnidosFil: Ge, Sundi. University of California at Los Angeles; Estados UnidosFil: Zhu, Yuhua. University of California at Los Angeles; Estados UnidosFil: Petrigliano, Frank A.. University of California at Los Angeles; Estados UnidosFil: Nsair, Ali. University of California at Los Angeles; Estados UnidosFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Xinmin. University of California at Los Angeles; Estados UnidosFil: Lyons, Karen M.. University of California at Los Angeles; Estados UnidosFil: Crooks, Gay M.. University of California at Los Angeles; Estados UnidosFil: McAllister, David R.. University of California at Los Angeles; Estados UnidosFil: Van Handel, Ben. Novogenix Laboratories; Estados UnidosFil: Adams, John S.. University of California at Los Angeles; Estados UnidosFil: Evseenko, Denis. University of California at Los Angeles; Estados Unido

Arts for All: 5th Year Review

In September 2002, the Los Angeles County Board of Supervisors adopted Arts for All: Los Angeles County Regional Blueprint for Arts Education, a ten-year strategic plan to restore arts education -- in dance, music, theatre, and the visual arts -- to the 1.7 million students in Los Angeles County's 80 school districts and Los Angeles County Office of Education (LACOE) classrooms. The Arts for All Executive Committee, with more than 100 partners and supported by the Los Angeles County Arts Commission, leads this effort. The initiative, now in its fifth year, has made great strides

Arts for All: 6 Year Review

In September 2002, the Los Angeles County Board of Supervisors adopted Arts for All: Los Angeles County Regional Blueprint for Arts Education, a strategic plan to restore arts education -- in dance, music, theatre, and the visual arts -- to the 1.7 million students in Los Angeles County's 81 school districts. The Arts for All Executive Committee, with more than 100 partners and managed by the Los Angeles County Arts Commission, leads this effort. The initiative, having completed its sixth year, has made great strides

Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells

The adaptive immune response to viral vectors reduces vector-mediated transgene expression from the brain. It is unknown, however, whether this loss is caused by functional downregulation of transgene expression or death of transduced cells. Herein, we demonstrate that during the elimination of transgene expression, the brain becomes infiltrated with CD4 and CD8 T cells and that these T cells are necessary for transgene elimination. Further, the loss of transgene-expressing brain cells fails to occur in the absence of IFNγ, perforin, and TNFα receptor. Two methods to induce severe immune suppression in immunized animals also fail to restitute transgene expression, demonstrating the irreversibility of this process. The need for cytotoxic molecules and the irreversibility of the reduction in transgene expression suggested to us that elimination of transduced cells is responsible for the loss of transgene expression. A new experimental paradigm that discriminates between downregulation of transgene expression and the elimination of transduced cells demonstrates that transduced cells are lost from the brain upon the induction of a specific antiviral immune response. We conclude that the anti-adenoviral immune response reduces transgene expression in the brain through loss of transduced cellsFil: Zirger, Jeffrey M.. Cedars Sinai Medical Center; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Puntel, Mariana. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bergeron, Josee. Cedars Sinai Medical Center; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Wibowo, Mia. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados UnidosFil: Moridzadeh, Rameen. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados UnidosFil: Bondale, Niyati. Cedars Sinai Medical Center; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Barcia, Carlos. Cedars Sinai Medical Center; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Kroeger, Kurt M.. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados UnidosFil: Liu, Chunyan. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados UnidosFil: Castro, Maria Graciela. University of California at Los Angeles. School of Medicine; Estados Unidos. Cedars Sinai Medical Center; Estados Unidos. University of Michigan; Estados UnidosFil: Lowenstein, Pedro R.. Cedars Sinai Medical Center; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados Unidos. University of Michigan; Estados Unido

Community Land Trust Feasibility in Los Angeles County

Los Angeles County is in the midst of a housing crisis. The Los Angeles County Affordable Housing Action identified community land trusts as one method to address the need for affordable housing for low- and moderate- income residents. This research examined current community land trust feasibility in Los Angeles County in consideration of the existing affordable housing funding and housing market environment. The research finds that community land trusts face significant funding gaps when trying to build or acquire housing for low- and moderate-income households. The one feasible option for community land trusts is new construction of large apartment buildings adjacent to transit, which qualifies the project for additional state funding. The research points to the need for additional funding directed towards CLTs for new construction projects and the creation of a pool of funding for CLTs to acquire existing housing in Los Angeles County

“I’d Rather Be in Afghanistan”: Antinomies of \u3cem\u3eBattle: Los Angeles\u3c/em\u3e

This article reads Battle: Los Angeles (2011) against the grain to argue that the film possesses an antiwar undertow running unexpectedly counter to its surface-level pro-military politics. The article uses the antinomy structuring Battle: Los Angeles as the opportunity to explore the pro- and anti-war politics of science fiction alien invasion film more generally, as well as consider the role of cooperation with the military in Hollywood blockbusters. The article closes with a Jamesonian reading of “the army”: as a kind of utopia as registered by mainstream cultural texts like Battle: Los Angeles

Intelligence of school children: Los Angeles as a case study 1922-1932

In an effort to construct the most advanced school system in the nation, Los Angeles school administrators and educators initiated a new scientific method of group intelligence testing. Almost immediately educators discovered serious limitations with the process and resisted its exclusive use. This study examines the reception of this new technology in Los Angeles between 1922 and 1932. Many historians have seen those associated with I.Q. measuring as bulwarks supporting the hegemony of Anglo-Saxon upper-middle class society. While their criticism has brought some non-equitable aspects of twentieth-century public education to surface, it has not led to our understanding of how educators interpreted the tests. An analysis of the sources, including reports published in the Department of Psychology and Education Research Bulletin of the Los Angeles City Schools, the Teachers' and Principals' School Journal, and the Minute~ of the Board of Education, provides insight into how Los Angeles educators viewed standardized testing

2005 Arts Education Performance Indicators Report

The mission of Arts for All: Los Angeles County Regional Blueprint for Arts Education is to establish sequential K-12 arts education in each of the 80 school districts in Los Angeles County and the Los Angeles County Office of Education (LACOE) classrooms.The Blueprint focuses on building an infrastructure to support districtwide education in dance, music, theatre and the visual arts. Progress is measured by five critical success factors.The data provided in this report gives a County-wide picture of the status of arts education with regard to five critical success factors in 2005

Information-theoretic Physical Layer Security for Satellite Channels

Shannon introduced the classic model of a cryptosystem in 1949, where Eve has access to an identical copy of the cyphertext that Alice sends to Bob. Shannon defined perfect secrecy to be the case when the mutual information between the plaintext and the cyphertext is zero. Perfect secrecy is motivated by error-free transmission and requires that Bob and Alice share a secret key. Wyner in 1975 and later I.~Csisz\'ar and J.~K\"orner in 1978 modified the Shannon model assuming that the channels are noisy and proved that secrecy can be achieved without sharing a secret key. This model is called wiretap channel model and secrecy capacity is known when Eve's channel is noisier than Bob's channel. In this paper we review the concept of wiretap coding from the satellite channel viewpoint. We also review subsequently introduced stronger secrecy levels which can be numerically quantified and are keyless unconditionally secure under certain assumptions. We introduce the general construction of wiretap coding and analyse its applicability for a typical satellite channel. From our analysis we discuss the potential of keyless information theoretic physical layer security for satellite channels based on wiretap coding. We also identify system design implications for enabling simultaneous operation with additional information theoretic security protocols

“‘Relentless Geography’: Los Angeles’ Imagined Cartographies in Karen Tei Yamashita’s Tropic of Orange,”

What would a map of Los Angeles drawn from the ground up look like? In his groundbreaking work The Production of Space (1974), Henri Lefebvre argues that the conceived space of urban planners is fundamentally distinct from lived space, which cannot be mapped out. In her impressive city-wide narrative, Karen Tei Yamashita’s Tropic of Orange (1997) demonstrates the effects of imposing conceived space upon the lived space of inner city Los Angeles residents, and what happens when the counter-model of space being lived by a city’s inhabitants rebels. Yamashita’s text mirrors this disjuncture between represented and lived space through the use of narrative surrealism. Space is magically reconfigured in the city, shrinking the uninhabited Downtown and expanding over-populated yet underrepresented neighborhoods, literally shifting geographically until its mapping matches the social space of those on the ground rather than those who map it from above. Literary criticism on the novel to date has largely interpreted Tropic of Orange as a commentary on the effects of globalization; not enough attention has been paid to the novel’s surreal expansion of Los Angeles’ inner city. Using Lefebvre’s “science of space,” anchored by Los Angeles’ city planning schemas, I argue that Yamashita offers a different map, one perceived by the subsets of LA’s population that fall through the cracks of the city’s grid. Tropic of Orange’s unorthodox formal structure, when combined with its narrative surrealism, creates a differential space in the text, transforming the novel into the Los Angeles imagined by its anonymous users