Genetic similarities of Escherichia coli isolated from different substrates of the broiler production chain

Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E. coli. Furthermore, 10 pairs of clones of E. coli were found always in the same substrate (two waterwater pairs; three soil-soil pairs and five meat-meat pairs) using PFGE. These findings suggest that certain strains of E. coli may have habitat preferences, making the transfer from one substrate type to another more difficult to occur. Moreover, since no clones were found between environmental samples and chicken meat, it is possible to imply a low risk of E. coli transmissibility throughout the chicken meat production chain

FATORES ASSOCIADOS AO EXCESSO DE PESO E RISCO PARA DOENÇAS CARDIOVASCULARES EM MULHERES CLIMATÉRICAS

Objetivo: avaliar a frequência de excesso de peso e fatores de risco para doenças cardiovasculares (DCV) em mulheres climatéricas não usuárias de Terapia de reposição hormonal (TRH) e sua associação com sintomatologia climatérica. Métodos: estudo transversal realizado com 48 mulheres, idade entre 40 e 65 anos de uma cidade do Paraná no ano de 2019. Coletou-se  dados sociodemográficos, clínicos e antropométricos. Avaliou-se a pressão arterial (PA), presença de síndrome metabólica (SM), perfil bioquímico e hormonal A intensidade dos sintomas climatéricos foi avaliada por meio do Índice Menopausal de Kupperman (IK). Resultados: Observou-se elevada   frequência de excesso de peso (54,2%), risco para DCV (52,9%) e de sintomas climatéricos moderados e graves (68,8%). Mulheres que relataram 2 gestações ou mais apresentaram maior frequência de excesso de peso (64,9%) e risco de DCV (62,2%) (p < 0,05). Mulheres com sintomas climatéricos moderados ou graves apresentaram maior frequência de PA acima do recomendado (84,6%) (p= 0,0137). Ainda, verificou-se que mulheres na pós-menopausa apresentaram níveis de colesterol total e LDL-colesterol significativamente elevados comparados às mulheres na pré e perimenopausa (p<0,05). Conclusão: O excesso de peso e o risco para DCV foram frequentes na amostra, especialmente nas mulheres na pós-menopausa e com sintomas climatéricos moderados e graves

Direct effect of p,p'- DDT on mice liver

ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame oil; Groups 2 and 3 received an i.p. dose of 50 and 100 µg/g p,p′-DDT respectively, dissolved in sesame oil. All groups were treated once daily for four days. Real-time PCR analysis of several genes was undertaken. Additionally, biochemical parameters and histopathological changes were measured. NQO1, HMOX1, NR1I3 and NR3C1 were up-regulated in DDT-exposed animals compared to the vehicle control group, while only SREBP1 was down-regulated in the 100 µg/g group. MTTP and FABP5, not previously reported for DDT exposure, but involved in regulation of fatty acid fluxes, could also function as biomarkers cross-talking between these signaling pathways. These results suggest that beyond epidemiological data, there is increasing molecular evidence that DDT may mimic different processes involved in diabetes and insulin resistance pathways

Resumos em andamento - Educação

Resumos em andamento - Educaçã

Resumos em andamento - Educação

Resumos em andamento - Educaçã