Communication Training of Future Sports Coaches in the Context of Neurophysiological Patterns

The article presents experimental verification of improving communicative training for future sports coaches in the context of neurophysiological patterns by expedient changes in pedagogical conditions, namely, forming a value attitude of students towards future professionally-oriented communicative activity; enhancing interactive learning methods; improving educational and methodical support; optimizing the subject-subject interaction of participants in the educational process in simulated situations of professional communication. The research aims to experimentally verify the above-mentioned pedagogical conditions and identify levels of indicators in future sports coaches’ preparedness for professionally oriented communicative activity. The research involved 105 students of the experimental group and 106 students of the control group, who voluntarily agreed to participate in the experiment. Research methods include the elaboration of the author’s didactic material, modelling of new pedagogical conditions and diagnostic methods. Diagnosis of levels of indicators in future sports coaches’ preparedness for professionally oriented communicative activity is carried out with the help of the diagnosed complex of both standard and adapted and modified or specially developed following criteria and indicators of the investigated phenomenon of author’s methods. After the formative experiment, the number of students with high and average levels of preparedness for professionally oriented communication activities has increased in EG (by 12.4% and 13.3%, respectively) and at the same time, the number of low-level students has decreased (by 25.7%). The results of the experimental work provide an opportunity to approve the effectiveness of the introduced pedagogical conditions of professionally-oriented communication training for future sports coaches.</p

колективна монографія

Кримінальний процесуальний кодекс 2012 року: ідеологія та практика правозастосування: колективна монографія / за заг. ред. Ю. П. Аленіна ; відпов. за вип. І. В. Гловюк. - Одеса : Видавничий дім «Гельветика», 2018. - 1148 с

Auxiliary elements of mammalian pre-mRNAs polyadenylation signals.

ABSTRACT Primary, secondary and higher-order structures of downstream elements of mammalian pre-mRNA polyadenylation signals [poly(A) signals] are reviewed. We have carried out a detailed analysis on our database of 244 human pre-mRNA poly(A) signals in order to characterize elements in their downstream regions. We suggest that the downstream region of the mammalian pre-mRNA poly(A) signal consists of various simple elements located at different distances from each other. Thus, the downstream region is not described by any precise consensus. Searching our database, we found that 80% of pre-mRNAs with the AAUAAA or AUUAAA core upstream elements contain simple downstream elements, consisting of U-rich and/or 2GU/U tracts, the former occurring~2-fold more often than the latter. Approximately one-third of the pre-mRNAs analyzed here contain sequences that may form G-quadruplexes. A substantial number of these sequences are located immediately downstream of the poly(A) signal. A possible role of G-rich sequences in the polyadenylation process is discussed. A model of the secondary structure of the SV40 late pre-mRNA poly(A) signal downstream region is presented

Structural insight into HIV-1 reverse transcription initiation in MAL-like templates (CRF01_AE, subtype G and CRF02_AG)

<div><p>Based on the known structural model for reverse transcription initiation complex of the human immunodeficiency virus type 1 (HIV-1) MAL isolate, we attempted to predict a structural behavior of MAL-like templates (CRF01_AE, subtype G and CRF02_AG) within the initiation complex by <i>in silico</i> experiments. Switches from the D-duplex (dimerization-competent) conformation to the I-duplex (initiation-competent) conformation and then to conformations with an open primer activation signal (PAS) structure have been examined for four fragments of U5 and primer binding site (PBS) region, the minimal fragment (nt 121–243), fragment 1 (nt 110–243), fragment 2 (nt 113–259), and extended fragment 2 (nt 109–261). Switches from the D-duplex conformation to the I-duplex conformation in the minimal fragment or fragment 1 and from the I-duplex conformation to conformations with exposed PAS motif in fragment 1 are similar in all MAL-like templates. A PAS exposure in fragment 2 and extended fragment 2 is supported by PBS stem extension which structure is affected by subtype-specific variations in CRF01_AE (the mutated motif ¹¹⁶GUUAG¹²⁰) and CRF02_AG (7-nt deletion downstream of the PBS motif and G/C/A insertion at the 3′ end of fragment 2). These switchable conformations contain the established structural elements essential for HIV-1 reverse transcription initiation as well as several elements that may also be relevant to initiation process, namely hairpins with GAAA apical loops and self-contained motifs of the duplicate insertion and the downstream palindromic sequence. Taken together, our findings suggest a role for the duplicate insertion of MAL-like templates in HIV-1 reverse transcription initiation process and possible mechanisms to realize this role.</p></div