ANALISIS DAN PERANCANGAN SISTEM BASISDATA BERBASISKAN WEB PADA SMU BHINNEKA TUNGGAL IKA

ANALISIS DAN PERANCANGAN SISTEM BASISDATA BERBASISKAN WEB PADA SMU BHINNEKA TUNGGAL IK

Generation of Human-Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells using Small-Molecule Compound VC6TFZ

BACKGROUND: Induced pluripotent stem cells (iPSCs) were generated from somatic cells through reprogramming process. Peripheral blood mononuclear cells (PBMNCs) were an attractive source cells due to the ease of accessibility, need minimal invasive procedure, and can be stored frozen. Small-molecule compound VC6TFZ has been successfully reprogrammed iPSCs from mouse fibroblast, but it has not been proven in human. AIM: This study aims to determine whether the small-molecule compound VC6TFZ can induce pluripotency of PBMNC to generate iPSCs. METHODS: Mononuclear cells were isolated from peripheral venous blood using centrifugation gradient density method. Mononuclear cells were cultured for 6 days in expansion medium and 48 h using hanging drop method. Pluripotency induction process using small-molecule compound VC6TFZ was done in 14 days then the medium changed to 2i medium for 7 days. Identification of iPSCs based on colony morphology and expression of pluripotency marker OCT4 and SOX2. RESULTS: Colonies appeared on day 9 of reprogramming process. These colonies had round, large, and cobble stone morphology like embryonic stem cell. These colonies had positive expression of pluripotency markers OCT4 and SOX2. All experimental groups had significantly higher expression of OCT4 and SOX2 than control group. CONCLUSION: Small-molecule compound VC6TFZ could induce pluripotency of human PBMNC to generate iPSCs

EFFECT OF PLATELET RICH PLASMA ON PROLIFERATION OF ENDOTHELIAL PROGENITOR CELL (EPC) OF STABLE CORONARY ARTERY DISEASE PATIENT

ABSTRACT Effect of Platelet Rich Plasma (PRP) on Proliferation of Endothelial Progenitor Cell (EPC) of Stable Coronary Artery Disease Patient Ronald R Hehanusa, Andrianto, Budi S Pikir   Background : Endothelial Progenitor Cell (EPC) is the progenitor of endothelial cell which has important role in  regulation of vascular wall integrity and homeostasis, to protect vessels from inflamation and thrombosis, that leads into pathogenesis of coronary artery disease. Growth factors proven has important role to stimulate transduction signal in the process of proliferation of EPC. Platelet Rich Plasma (PRP) contains variety of growth factors, wellknown role in homeostasis and wound healing process. Therefore, this study was conducted to analyze the effect of PRP on proliferation of EPC of Stable Coronary Artery Disease (SCAD) patient. Objective : To analyze the effect of Platelet Rich Plasma (PRP) on the proliferation of Endothelial Progenitor Cell (EPC) from peripheral blood of patient with SCAD    Methods : This is an in vitro, true experimental, post-test only control group design. The mononuclear cells were isolated from peripheral blood of SCAD patient and cultured in M-199 medium. EPC divided into 3 groups, which received Platelet Rich Plasma (PRP), Platelet Poor Plasma (PPP), and control. After 14 days of incubation, immunocytochemical examination was performed, EPC which marked with CD34, FITC labeled,was counted using immunofluoroscence microscope. Data analysis using ANOVA test. Result : Cell counting showed significant increase of EPC proliferation in PRP group compared to PPP group (1.052 ± 0.16 vs 0.762 ± 0.19, p = 0.003), and control group as well (1.052 ± 0.16 vs 0.068 ± 0.05, p = 0.000). EPC proliferation in PPP group also increase significantly compared to control group (0.762 ± 0.19 vs 0.068 ± 0.05, p = 0.000). Conclusion : Platelet Rich Plasma (PRP) increase EPC proliferation significantly from peripheral blood of SCAD patient.   Keywords : EPC proliferation, PRP, SCA

Peningkatan Keaktivan Belajar Melalui Strategi Think Talk Write (TTW) Pada Mata Pelajaran IPA Siswa Kelas IV SDN Teloyo III Kecamatan Wonosari Klaten Tahun Pelajaran 2012/2013

Penelitian dengan rumusan masalah “Apakah strategi pembelajaran think talk write (TTW) dapat meningkatkan keaktivan belajar pada mata pelajaran IPA kelas IV SD Negeri Teloyo III Wonosari Klaten Tahun Ajaran 2012 / 2013”? Tujuan penelitian ini adalah untuk meningkatkan keaktivan belajar siswa melalui penerapan strategi pembelajaran Think Talk Write (TTW) pada siswa kelas IV SD Negeri Teloyo III Kecamatan Wonosari Klaten. Jenis Penelitian ini adalah Penelitian Tindakan Kelas. Subyek penelitian ini adalah siswa kelas IV SD Negeri Teloyo III Kecamatan Wonosari Klaten yang berjumlah 17 siswa (12 siswa lakilaki dan 5 siswa perempuan). Metode pengumpulan data menggunakan lembar wawancara, lembar observasi, dokumentasi dan tes evaluasi serta lembar kerja siswa. sedangkan data dianalisa menggunakan Teknik analisis data yang digunakan dengan menggunakan teknik analisis data deskriptif kualitatif yang meliputi tahap pengumpulan data, reduksi data, penyajian data dan verifikasi data. Penelitian tindakan kelas ini dilakukan melalui dua siklus. Dari hasil tindakan siklus I sampai siklus II terjadi peningkatan keaktivan pada diri siswa. Hal itu terlihat dari hasil penelitian dan didukung oleh fakta – fakta sebagai berikut : (1) keaktivan bertanya dengan guru atau teman tentang hal yang belum dipahami (2) keaktivan mengemukakan pendapat, gagasan atau ide (3) keaktivan membuat catatan individu (4) keaktivan bekerjasama dalam kelompok diskusi (5) keaktivan mengerjakan tugas dan soal individu. Dari semua indikator tersebut, keaktivan siswa secara umum mengalami peningkatan dari kondisi awal siswa yang tergolong aktif 3 siswa atau sebesar 17,64% meningkat pada siklus I menjadi 8 siswa atau sebesar 47,06% dan pada siklus II keaktivan siswa meningkat meningkat menjadi 13 siswa atau sebesar 76,47%.. Dengan meningkatnya keaktivan siswa maka berpengaruh pada hasil belajar siswa, dari prasiklus 29,41% atau hanya 5 siswa yang tuntas KKM meningkat menjadi 47,06% atau 8 siswa yang tuntas pada siklus I, kemudian meningkat lagi menjadi 82,35% atau 14 siswa yang tuntas pada siklus II. Penelitian ini menyimpulkan bahwa penerapan strategi Think Talk Write dapat meningkatkan keaktivan belajar IPA pada siswa kelas IV SD Negeri Teloyo III Kecamatan Wonosari Klaten tahun pelajaran 2012/2013

THE DIFFERENCE OF BLOOD PRESSURE AND ARTERIAL STIFFNESS AFTER INTAKE OF ARABICA AND ROBUSTA COFFEE IN CONTROLLED HYPERTENSION

Potential mechanisms of caffeine promote large artery stiffness and associated with increased morbidity and mortality of cardiovascular disease. To find the difference of blood pressure and arterial stiffness after intake of arabica and robusta coffee in controlled hypertension, a quasi-experimental study was done enrolling 24 controlled hypertension and 24 normotensive subjects collected by purposive sampling. Subjects received ± 10,6 g caffeinated coffee in 150 ml water with crossover protocol twice in 5 days. The assessment of arterial stiffness by obtaining ultrasound 2D image of common carotid artery then was calculated with beta stiffness index. Blood pressure and arterial stiffness was measured before coffee intake and again at 30 to 60 minutes thereafter. In controlled hypertension, significant differences were found between initial systolic blood pressure (SBP), 30 and 60 minutes after arabica (p 0,002) and robusta (p 0,012) intake; between diastolic blood pressure (DBP), 30 and 60 minutes after of arabica (p 0,004) and robusta (p 0,025) oral administration; and between initial beta stiffness index, 30 and 60 minutes after arabica (p 0,018) oral administration. However, no significant difference was found between initial beta stiffness index, 30 and 60 minutes after robusta (p 0,104) oral administration. No significant difference was found in all variables after arabica and robusta intake. Significant differences were found in blood pressure after arabica or robusta intake and in arterial stiffness after arabica intake. However, no significant differences were found in arterial stiffness after robusta intake and in all variables after arabica and robusta intake

Uji Dosis Tepung Buah Sirih Hutan (Piper Aduncum L.) Terhadap Mortalitas Hama Sitophilus Oryzae L. Pada Beras Di Penyimpanan

Research aims to obtain dose of spiked pepper fruit flour (Piper aduncum L.) better of mortality pests imago S. oryzae on the rice. Research conducted at the laboratory of Plant Pests, Faculty of Agriculture, University of Riau from May to June 2015. The Research was conducted experimentally by using completely randomized design (CRD), which consists of 5 treatments, where each treatments was repeated 4 times to obtain 20 units experiment. The treatment used in the research are (0 g/ 100 g, 2 g/ 100 g, 4 g/ 100 g, 6 g/ 100 g, 8 g/ 100 g of rice). Data obtained analyzed statistically using analysis of variance followed by least significant difference (LSD) at 5%. Dose applications of spiked pepper fruit flour at doses 6 g/ 100 g rice better of imago S. oryzae with lethal time50 24,5 hour and mortality by 97,5% inhibit the growth of the population, shrinkage weight of rice, and lethal dose 95 by 4,5%. Keywoards: Dose, insecticide, Piper aduncum L., Sitophilus oryzae, rice

PENGARUH ARUS KENDARAAN KE JEMBATAN MAHAKAM KOTA (MAHKOTA) TERHADAP KEMACETAN PADA RUAS JALAN R.OTTO ISKANDARDINATA KOTA SAMARINDA

Jalan R.Otto Iskandardinata di Samarinda merupakan jalan arteri sekunder yang menghubungkan kawasan primer dan sekunder yang satu ke kawasan yang lainnya. Kondisi guna lahan yang terdapat pada ruas jalan Jendral Sudirman didominasi oleh bangunan umum dengan aktivitas umum yaitu perdagangan Jalan R.Otto Iskandardinata di Samarinda ini termasuk kategori jalan kelas III C. Seiring berkembangnya ekonomi dan naiknya tingkat kemakmuran penduduk akan mengakibatkan bertambahnya tingkat perjalanan Lalu lintas, yang terjadi akibat adanya kebutuhan akan transportasi dari masyarakat, dimana masyarakat akan selalu mencari jalan yang lebih cepat, aman dan lancar. Kenaikan jumlah penduduk dan banyaknya urbanisasi ke daerah perkotaan maka akan menimbulkan tingkat pergerakan dan kepadatan, sehingga kebutuhan akan transportasi pun meningkat pula. Atas dasar inilah, maka di lakukan penelitian bagaimana pengaruh arus kendaraan ke jembatan mahakam kota ( mahkota ) terhadap kemacetan pada ruas jalan R. Otto Iskandardinata kota samarinda. Adapun dalam pengaruh arus kendaraan ke jembatan mahakam kota ( mahkota ) terhadap kemacetan pada ruas jalan R. Otto Iskandardinata kota samarinda ini menggunakan Manual Kapasitas Jalan Indonesia (MKJI’1997), yang di uraikan berdasarkan Formulir UR-1 berupa Kondisi umum dan Geometrik jalan, UR-2 berupa data masukan lanjutan yaitu Arus dan Komposisi lalu lintas dan Hambatan samping, UR-3 berupa Analisa Kecepatan arus bebas kendaraan ringan, Kapasitas dan Kecepatan kendaraa