Numerical research of heated up to high temperatures particle influence on human skin

Numerical research results of heated to high temperatures particle influence on human skin are presented. The problem is solved in two-dimensional statement in Cartesian system of coordinates. The typical range of influence parameters of heated particle is considered. Temperature distributionы in different moments of time are obtained

Elaborated Action of the Human Primosome

The human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase α, which initiates genome replication by synthesizing chimeric RNA-DNA primers for DNA polymerases δ and ϵ. Accumulated biochemical and structural data reveal the complex mechanism of concerted primer synthesis by two catalytic centers. First, primase generates an RNA primer through three steps: initiation, consisting of dinucleotide synthesis from two nucleotide triphosphates; elongation, resulting in dinucleotide extension; and termination, owing to primase inhibition by a mature 9-mer primer. Then Polα, which works equally well on DNA:RNA and DNA:DNA double helices, intramolecularly catches the template primed by a 9mer RNA and extends the primer with dNTPs. All primosome transactions are highly coordinated by autoregulation through the alternating activation/inhibition of the catalytic centers. This coordination is mediated by the small C-terminal domain of the primase accessory subunit, which forms a tight complex with the template:primer, shuttles between the primase and DNA polymerase active sites, and determines their access to the substrate

Numerical research of heated up to high temperatures particle influence on human skin

Numerical research results of heated to high temperatures particle influence on human skin are presented. The problem is solved in two-dimensional statement in Cartesian system of coordinates. The typical range of influence parameters of heated particle is considered. Temperature distributionы in different moments of time are obtained

Elaborated Action of the Human Primosome

The human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase α, which initiates genome replication by synthesizing chimeric RNA-DNA primers for DNA polymerases δ and ϵ. Accumulated biochemical and structural data reveal the complex mechanism of concerted primer synthesis by two catalytic centers. First, primase generates an RNA primer through three steps: initiation, consisting of dinucleotide synthesis from two nucleotide triphosphates; elongation, resulting in dinucleotide extension; and termination, owing to primase inhibition by a mature 9-mer primer. Then Polα, which works equally well on DNA:RNA and DNA:DNA double helices, intramolecularly catches the template primed by a 9mer RNA and extends the primer with dNTPs. All primosome transactions are highly coordinated by autoregulation through the alternating activation/inhibition of the catalytic centers. This coordination is mediated by the small C-terminal domain of the primase accessory subunit, which forms a tight complex with the template:primer, shuttles between the primase and DNA polymerase active sites, and determines their access to the substrate

Spatial Mathematical Model of Heat Transfer in Human Skin Influenced by Heated up to High Temperatures Particle

Numerical research results of heat transfer in system “air-heated particle-skin layer” presented. Skin was influenced by heated up to high temperatures particle. The problem is solved in tree-dimensional statement in Cartesian system of coordinates. The typical range of influence parameters of heated particle considered. Temperature distributions in different moments of time obtained. Condition of burn occurrence by heated particle is under consideration in this research

Crop correlations with structure elements in varieties of grain sorghum of various ecological and geographical origin

In the conditions of the Donbass region, 38 varieties of grain sorghum of various ecological and geographical origin-breeding companies of Western Europe (“RAGT SEMENCES”, “EURALIS SEMENCES”, “PIONEER”), Ukraine, Russia, the international company “ADVANTA”, the American company “RICHARDSON SEED” were studied. Field research was carried out in the period from 2016 to 2018 in the experimental field of the Lugansk National Agrarian University on ordinary shallow weakly washed blackearth (chernozem) on loess-loam. It was found that the most adapted and productive (6,0 t/ha of grain or more) were early – maturing hybrids – Swift and Queyras; middle early – Solarius, PR88Y20, Bianca, Puma Star; mid-season - Bounty. The most productive (5,0 t/ha and more grain) varieties – Odessky 205, Krupinka 10, Darunok, Zersta 97. The average positive correlation between the duration of the growing season of varieties and crop yield was established. The density of the productive stem in the range from 11 to 25 panicles per 1 m2, the length of the panicle leg and the weight of 1000 grains did not have a significant correlation with the level of sorghum yield. Sorghum yield had an average and increased correlation with panicle weight, grain weight per panicle, number of grains per panicle, grain type, and medium negative relationship with plant height

Spatial Mathematical Model of Heat Transfer in Human Skin Influenced by Heated up to High Temperatures Particle

Numerical research results of heat transfer in system “air-heated particle-skin layer” presented. Skin was influenced by heated up to high temperatures particle. The problem is solved in tree-dimensional statement in Cartesian system of coordinates. The typical range of influence parameters of heated particle considered. Temperature distributions in different moments of time obtained. Condition of burn occurrence by heated particle is under consideration in this research

Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ

The cloning, expression, purification and crystallization of the complex of the second and third regulatory subunits of human Pol δ are reported. The crystals were characterized and an X-ray diffraction data set was collected to a resolution of 3 Å

Comment on “The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport”

O’Brien et al. (Research Article, 24 February 2017, eaag1789) proposed a novel mechanism of primase function based on redox activity of the iron-sulfur cluster buried inside the C-terminal domain of the large primase subunit (p58C). Serious problems in the experimental design and data interpretation raise concerns about the validity of the conclusions.National Institute of General Medical Sciences grant GM101167 to T.H.T.Peer Reviewe