Arthropod segmentation.

There is now compelling evidence that many arthropods pattern their segments using a clock-and-wavefront mechanism, analogous to that operating during vertebrate somitogenesis. In this Review, we discuss how the arthropod segmentation clock generates a repeating sequence of pair-rule gene expression, and how this is converted into a segment-polarity pattern by 'timing factor' wavefronts associated with axial extension. We argue that the gene regulatory network that patterns segments may be relatively conserved, although the timing of segmentation varies widely, and double-segment periodicity appears to have evolved at least twice. Finally, we describe how the repeated evolution of a simultaneous (Drosophila-like) mode of segmentation within holometabolan insects can be explained by heterochronic shifts in timing factor expression plus extensive pre-patterning of the pair-rule genes.BBSRC grant BB/P009336/1 in Cambridge (MA and EC) BBSRC grant BB/L020092/1 in Leeds (ADP) Trinity College JRF to Erik Clark in Cambridge EMBO Long term fellowship to Erik Clark in Harvar

Chemistry by Mobile Phone (or how to justify more time at the bar)

By combining automatic environment monitoring with Java smartphones a system has been produced for the real-time monitoring of experiments whilst away from the lab. Changes in the laboratory environment are encapsulated as simple XML messages, which are published using an MQTT compliant broker. Clients subscribe to the MQTT stream, and produce a user display. An MQTT client written for the Java MIDP platform, can be run on a smartphone with a GPRS Internet connection, freeing us from the constraints of the lab. We present an overview of the technologies used, and how these are helping chemists make the best use of their time

Effects of human recombinant growth hormone on exercise capacity, cardiac structure, and cardiac function in patients with adult-onset growth hormone deficiency

Objective Epidemiological studies suggest that adult-onset growth hormone deficiency (AGHD) might increase the risk of death from cardiovascular causes. Methods This was a 6-month double-blind, placebo-controlled, randomised, cross-over trial followed by a 6-month open-label phase. Seventeen patients with AGHD received either recombinant human growth hormone (rGH) (0.4 mg injection daily) or placebo for 12 weeks, underwent washout for 2 weeks, and were then crossed over to the alternative treatment for a further 12 weeks. Cardiac magnetic resonance imaging, echocardiography, and cardiopulmonary exercise testing were performed at baseline, 12 weeks, 26 weeks, and the end of the open phase (12 months). The results were compared with those of 16 age- and sex-matched control subjects. Results At baseline, patients with AGHD had a significantly higher systolic blood pressure, ejection fraction, and left ventricular mass than the control group, even when corrected for body surface area. Treatment with rGH normalised the insulin-like growth factor 1 concentration without an effect on exercise capacity, cardiac structure, or cardiac function. Conclusion Administration of rGH therapy for 6 to 9 months failed to normalise the functional and structural cardiac differences observed in patients with AGHD when compared with a control group

An algebraic model for inversion and deletion in bacterial genome rearrangement

Reversals are a major contributor to variation among bacterial genomes, with studies suggesting that reversals involving small numbers of regions are more likely than larger reversals. Deletions may arise in bacterial genomes through the same biological mechanism as reversals, and hence a model that incorporates both is desirable. However, while reversal distances between genomes have been well studied, there has yet to be a model which accounts for the combination of deletions and short reversals. To account for both of these operations, we introduce an algebraic model that utilises partial permutations. This leads to an algorithm for calculating the minimum distance to the most recent common ancestor of two bacterial genomes evolving by short reversals and deletions. The algebraic model makes the existing short reversal models more complete and realistic by including deletions, and also introduces new algebraic tools into evolutionary distance problems.Comment: 19 pages, 10 figure

Detrital zircon geochronology and evolution of the Nacimiento block late Mesozoic forearc basin, central California coast

Forearc basins are first-order products of convergent-margin tectonics, and their sedimentary deposits offer unique perspectives on coeval evolution of adjacent arcs and subduction complexes. New detrital zircon U-Pb geochronologic data from 23 sandstones and 11 individual conglomerate clasts sampled from forearc basin strata of the Nacimiento block, an enigmatic stretch of the Cordilleran forearc exposed along the central California coast, place constraints on models for forearc deformation during evolution of the archetypical Cordilleran Mesozoic margin. Deposition and provenance of the Nacimiento forearc developed in three stages: (1) Late Jurassic– Valanginian deposition of lower Nacimiento forearc strata with zircon derived from the Jurassic–Early Cretaceous arc mixed with zircon recycled from Neoproterozoic– Paleozoic and Mesozoic sedimentary sources typical of the continental interior; (2) erosion or depositional hiatus from ca. 135 to 110 Ma; and (3) Albian–Santonian deposition of upper Nacimiento forearc strata with zircon derived primarily from the Late Cretaceous arc, accompanied by Middle Jurassic zircon during the late Albian–Cenomanian. These data are most consistent with sedimentary source terranes and a paleogeographic origin for the Nacimiento block south of the southern San Joaquin Basin in southern California or northernmost Mexico. This interpreted paleogeographic and depositional history of the Nacimiento block has several implications for the tectonic evolution of the southern California Mesozoic margin. First, the Nacimiento forearc depositional history places new timing constraints on the Early Cretaceous unconformity found in forearc basin strata from the San Joaquin Valley to Baja California. This timing constraint suggests a model in which forearc basin accommodation space was controlled by accretionary growth of the adjacent subduction complex, and where tectonic events in the forearc and the arc were linked through sediment supply rather than through orogenic-scale wedge dynamics. Second, a paleogeographic origin for the Nacimiento forearc south of the southern San Joaquin Valley places new constraints on end-member models for the kinematic evolution of the Sur-Nacimiento fault. Although this new paleogeographic reconstruction cannot distinguish between sinistral strike-slip and thrust models, it requires revision of existing sinistral-slip models for the Sur-Nacimiento fault, and it highlights unresolved problems with the thrust model