Adaptive Significance of Aberrant Chickadee Song in Illinois

I studied the behavioral responses of Black-capped (Poecile atricapilus) and Carolina (P. carolinensis) chickadees to playback of Black-capped, Carolina, and aberrant chickadee songs throughout the state of Illinois. These two species are parapatrically distributed throughout the eastern United States, including Illinois. Some areas exist where the ranges of the two species overlap. Individuals in these contact zones often sing aberrant vocalizations. This study focused on the two parental vocalizations (i.e., Black-capped and Carolina song), and two of the most common aberrant vocalizations (the \u27Greenville\u27 and \u27Vandalia\u27 aberrant dialects), in an attempt to understand the adaptive significance of these aberrant songs. Playback experiments were conducted from March to June in 2011 and 2012 in 28 counties throughout Illinois. During playback trials, behavioral response data were collected and compared among the four different chickadee playback vocalizations; Black-capped, Carolina, and Greenville and Vandalia aberrant dialects. Black-capped and Carolina chickadees displayed their most aggressive responses to conspecific playback. However, contact zone individuals responded aggressively to all playback types. Aberrant vocalizations elicited largely intermediate responses from Black-capped and Carolina Chickadees. We found no differences in behavioral responses to playback when comparing responses of parental populations within 50 km and farther than 50 km from the contact zone. Due to the numerous aberrant dialects and parental songs that exist throughout the contact zones, it may be necessary for contact zone individuals to recognize and respond to most chickadee-like song types in order to establish and defend territories, and to attract mates. Intermediate responses to aberrant vocalizations by parental birds could be due to certain syllabic cues within the aberrant songs that elicit aggressive response

Climate Change and Coral Reef Bleaching: An Ecological Assessment of Long-Term Impacts, Recovery Trends and Future Outlook

Since the early 1980s, episodes of coral reef bleaching and mortality, due primarily to climate-induced ocean warming, have occurred almost annually in one or more of the world\u27s tropical or subtropical seas. Bleaching is episodic, with the most severe events typically accompanying coupled ocean–atmosphere phenomena, such as the El Niño-Southern Oscillation (ENSO), which result in sustained regional elevations of ocean temperature. Using this extended dataset (25+ years), we review the short- and long-term ecological impacts of coral bleaching on reef ecosystems, and quantitatively synthesize recovery data worldwide. Bleaching episodes have resulted in catastrophic loss of coral cover in some locations, and have changed coral community structure in many others, with a potentially critical influence on the maintenance of biodiversity in the marine tropics. Bleaching has also set the stage for other declines in reef health, such as increases in coral diseases, the breakdown of reef framework by bioeroders, and the loss of critical habitat for associated reef fishes and other biota. Secondary ecological effects, such as the concentration of predators on remnant surviving coral populations, have also accelerated the pace of decline in some areas. Although bleaching severity and recovery have been variable across all spatial scales, some reefs have experienced relatively rapid recovery from severe bleaching impacts. There has been a significant overall recovery of coral cover in the Indian Ocean, where many reefs were devastated by a single large bleaching event in 1998. In contrast, coral cover on western Atlantic reefs has generally continued to decline in response to multiple smaller bleaching events and a diverse set of chronic secondary stressors. No clear trends are apparent in the eastern Pacific, the central-southern-western Pacific or the Arabian Gulf, where some reefs are recovering and others are not. The majority of survivors and new recruits on regenerating and recovering coral reefs have originated from broadcast spawning taxa with a potential for asexual growth, relatively long distance dispersal, successful settlement, rapid growth and a capacity for framework construction. Whether or not affected reefs can continue to function as before will depend on: (1) how much coral cover is lost, and which species are locally extirpated; (2) the ability of remnant and recovering coral communities to adapt or acclimatize to higher temperatures and other climatic factors such as reductions in aragonite saturation state; (3) the changing balance between reef accumulation and bioerosion; and (4) our ability to maintain ecosystem resilience by restoring healthy levels of herbivory, macroalgal cover, and coral recruitment. Bleaching disturbances are likely to become a chronic stress in many reef areas in the coming decades, and coral communities, if they cannot recover quickly enough, are likely to be reduced to their most hardy or adaptable constituents. Some degraded reefs may already be approaching this ecological asymptote, although to date there have not been any global extinctions of individual coral species as a result of bleaching events. Since human populations inhabiting tropical coastal areas derive great value from coral reefs, the degradation of these ecosystems as a result of coral bleaching and its associated impacts is of considerable societal, as well as biological concern. Coral reef conservation strategies now recognize climate change as a principal threat, and are engaged in efforts to allocate conservation activity according to geographic-, taxonomic-, and habitat-specific priorities to maximize coral reef survival. Efforts to forecast and monitor bleaching, involving both remote sensed observations and coupled ocean–atmosphere climate models, are also underway. In addition to these efforts, attempts to minimize and mitigate bleaching impacts on reefs are immediately required. If significant reductions in greenhouse gas emissions can be achieved within the next two to three decades, maximizing coral survivorship during this time may be critical to ensuring healthy reefs can recover in the long term

Maternal tobacco, cannabis and alcohol use during pregnancy and risk of adolescent psychotic symptoms in offspring

Background Adverse effects of maternal substance use during pregnancy on fetal development may increase risk of psychopathology. Aims To examine whether maternal use of tobacco, cannabis or alcohol during pregnancy increases risk of offspring psychotic symptoms. Method A longitudinal study of 6356 adolescents, age 12, who completed a semi-structured interview for psychotic symptoms in the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort. Results Frequency of maternal tobacco use during pregnancy was associated with increased risk of suspected or definite psychotic symptoms (adjusted odds ratio 1.20, 95% CI 1.05–1.37, P = 0.007). Maternal alcohol use showed a non-linear association with psychotic symptoms, with this effect almost exclusively in the offspring of women drinking >21 units weekly. Maternal cannabis use was not associated with psychotic symptoms. Results for paternal smoking during pregnancy and maternal smoking post-pregnancy lend some support for a causal effect of tobacco exposure in utero on development of psychotic experiences. Conclusions These findings indicate that risk factors for development of non-clinical psychotic experiences may operate during early development. Future studies of how in utero exposure to tobacco affects cerebral development and function may lead to increased understanding of the pathogenesis of psychotic phenomena

Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction.

Changes in lattice structure across sub-regions of protein crystals are challenging to assess when relying on whole crystal measurements. Because of this difficulty, macromolecular structure determination from protein micro and nanocrystals requires assumptions of bulk crystallinity and domain block substructure. Here we map lattice structure across micron size areas of cryogenically preserved three-dimensional peptide crystals using a nano-focused electron beam. This approach produces diffraction from as few as 1500 molecules in a crystal, is sensitive to crystal thickness and three-dimensional lattice orientation. Real-space maps reconstructed from unsupervised classification of diffraction patterns across a crystal reveal regions of crystal order/disorder and three-dimensional lattice tilts on the sub-100nm scale. The nanoscale lattice reorientation observed in the micron-sized peptide crystal lattices studied here provides a direct view of their plasticity. Knowledge of these features facilitates an improved understanding of peptide assemblies that could aid in the determination of structures from nano- and microcrystals by single or serial crystal electron diffraction

Purification and Identification of a 7.6-kDa Protein in Media Conditioned by Superinvasive Cancer Cells

Background: Selection of the human drug sensitive and invasive cell line (MDA-MB-435S-F) with the chemotherapeutic agent paclitaxel, resulted in the development of drug resistant cell lines displaying enhanced invasion-related characteristics. Materials and Methods: Serum-free conditioned media from the human cancer drug-sensitive and invasive cell line (MDA-MB-435S-F) and its paclitaxel-resistant superinvasive variant (MDA-MB-435S-F/Taxol10p4pSI) were analyzed using Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Results: A differentially expressed protein was observed at 7.6 kDa, which was 4-fold upregulated in MDA-MB-435S-F/Taxol10p4pSI. The differentially expressed protein was identified using matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS), as a fragment of bovine transferrin. The transferrin receptor was also found to be overexpressed in the superinvasive cell line. Conclusion: Cleavage of serum proteins such as transferrin could provide a valuable source of markers for malignant tumours and could also play a role in aspects of cancer pathogenesis, such as tumour cachexia

The Nature of Boulder-Rich Deposits in the Upper Big Flat Brook Drainage, Sussex County, New Jersey

The upper reaches of the Big Flat Brook drainage, northwest of Kittatinny Mountain, contain a variety of glacial, pro-glacial, and periglacial deposits from the Late Quaternary. The area is dominated by recessional moraines and ubiquitous ground moraine, along with meltwater deposits, drumlins, and possible postglacial periglacial features. We have identified a curious boulder-rich deposit in the vicinity of Lake Ocquittunk and Lake Wapalanne on upper Big Flat Brook. The area where these boulder deposits occur is mapped (1:24,000 surficial geology) as till. As mapped and observed, larger cobbles and boulders within the till are quartz-pebble conglomerate, quartzite, sandstone, and shale. The boulder-rich deposits differ from the typical till, however. Unlike the local till, which is more mixed in lithology, the boulder deposits are nearly exclusively Shawangunk conglomerate. The deposits are discontinuous, but appear to occur at a topographic level above the meltwater stream terraces. The boulders in the deposits lie partially embedded in soil, but are very closely spaced. The boulders range in size from ~20cm to over 100cm, and present a subrounded to subangular shape. There appears to be a fabric orientation of the boulders, NE-SW, with subsidiary orientations. As the boulder deposits differ from other mapped features in the area, we attempt to ascertain the origin for the deposits. Unique moraine dynamics, meltwater flood, periglacial slope movement, and slope-snow bank processes are possible explanations. Detailed examination of these boulder deposits will provide a finer explanation of the glacial and post-glacial geomorphic history of the area

The effect of HIV infection on time off work in a large cohort of gold miners with known dates of seroconversion

Objectives To estimate the effect of HIV infection on time off work. To provide baseline estimates for economic and actuarial models, and for evaluations of ART and other workplace interventions.Methods A retrospective cohort study of gold miners with known dates of seroconversion to HIV, and an HIV-negative comparison group, used routinely collected data to estimate the proportion of time off work by calendar period (1992-2002, prior to the introduction of ART), age, time since seroconversion and period before death. The authors calculated ORs for overall time off work and RR ratios (RRR, using multinomial logistic regression) for reasons off work relative to being at work.Results 1703 HIV-positive and 4859 HIV-negative men were followed for 34 424 person-years. HIV-positive miners spent a higher proportion of time off work than negative miners (20.7% vs 16.1%) due to greater medical and unauthorised absence. Compared with HIV-negative miners, overall time off work increased in the first 2 years after seroconversion (adjusted OR 1.40 (95% CI 1.36 to 1.45)) and then remained broadly stable for a number years, reaching 38.8% in the final year before death (adjusted OR 3.27, 95% CI 2.95 to 3.63). Absence for medical reasons showed the strongest link to HIV infection, increasing from an adjusted RRR of 2.66 (95% CI 2.45 to 2.90) for the first 2 years since seroconversion to 13.6 (95% CI 11.8 to 15.6) in the year prior to death.Conclusions Time off work provides a quantifiable measure of the effect of HIV on overall morbidity. HIV/AIDS affects both labour supply (increased time off work) and demand for health services (increased medical absence). The effects occur soon after seroconversion and stabilise before reaching very high levels in the period prior to death. Occupational health services are an important setting to identify HIV-infected men early

Gundalf the Magical Pistol (formerly "Pete's Pistol")

Gundalf is a weapon for the MAGE 2.0 Game System whose main purpose is to fire IR spell packets. Gundalf additionally features an internal, rechargeable, LiPo battery with a 2+ hour battery life, a player-facing speaker that provides audio feedback, an LCD screen, an LED ring that provide visual feedback, and a BlueTooth 2.0 module that communicates with the MAGE 2.0 game server

Contact with Caregivers is Associated with Composition of the Infant Gastrointestinal Microbiome in the First 6 Months of Life

Objectives Little is known about how physical contact at birth and early caregiving environments influence the colonization of the infant gastrointestinal microbiome. We investigated how infant contact with caregivers at birth and within the first 2 weeks of life relates to the composition of the gastrointestinal microbiome in a sample of U.S. infants (n = 60). Methods Skin-to-skin and physical contact with caregivers at birth and early caregiving environments were surveyed at 2 weeks postpartum. Stool samples were collected from infants at 2 weeks, 2, 6, and 12 months of age and underwent 16S rRNA sequencing as a proxy for the gastrointestinal microbiome. Associations between early caregiving environments and alpha and beta diversity, and differential abundance of bacteria at the genus level were assessed using PERMANOVA, and negative binomial mixed models in DEseq2. Results Time in physical contact with caregivers explained 10% of variation in beta diversity at 2 weeks\u27 age. The number of caregivers in the first few weeks of life explained 9% of variation in beta diversity at 2 weeks and the number of individuals in physical contact at birth explained 11% of variation in beta diversity at 6 months. Skin-to-skin contact on the day of birth was positively associated with the abundance of eight genera. Infants held for by more individuals had greater abundance of eight genera. Discussion Results reveal a potential mechanism (skin-to-skin and physical contact) by which caregivers influence the infant gastrointestinal microbiome. Our findings contribute to work exploring the social transmission of microbes

Selection of HyspIRI optimal band positions for the earth compositional mapping using HyTES data

The National Aeronautics and Space Administration (NASA) has proposed the launch of a new space-borne sensor called HyspIRI (Hyperspectral and Infrared Imager) which will cover the spectral range from 0.4–12 μm. Two instruments will be mounted on HyspIRI platform: 1) a hyperspectral instrument which can sense earth surface between 0.4 and 2.5 μm at 10 nm intervals and 2) a multispectral infrared sensor will acquire images between 3 and 12 μm in eight spectral bands (one in Mid infrared (MIR) and seven in Thermal Infrared (TIR)). The TIR spectral wavebands will be positioned based on their importance in various applications. This study aimed to identify HyspIRI optimal TIR wavebands position for earth compositional mapping. A Genetic Algorithm coupled with the Spectral Angle Mapper (GA-SAM) was used as a spectral bands selector. High dimensional HyTES (Hyperspectral Thermal Emission Spectrometer) emissivity spectra comprised of 202 spectral bands of Cuprite and Death Valley regions were used to select meaningful subsets of bands for earth compositional mapping. The GA-SAM was trained for fifteen mineral classes and the algorithms were run iteratively 50 times. High calibration (> 95%) and validation (> 90%) accuracies were achieved with a limited number (seven) of spectral bands selected by GA-SAM. The knowledge of important band positions will help the scientists of the HyspIRI group to place spectral bands in regions where accuracies of earth compositional mapping can be enhanced