30 research outputs found
Doença de Chagas: caracterização de formas clĂnicas e estratificação do risco de morte no Oeste do Estado do Rio Grande do Norte
O perfil clĂnico com classificação das formas clĂnicas e a gravidade da doença de
Chagas foram avaliados em indivĂduos sororreativos para o Trypanosoma cruzi
procedentes da mesorregiĂŁo Oeste Potiguar. Neste estudo transversal foram
incluĂdos 186 indivĂduos adultos, entre 23 a 78 anos de idade. Todos os
indivĂduos foram submetidos Ă avaliação clĂnica, aplicado um questionário clĂnicoepidemiolĂłgico
e realizados eletrocardiograma de repouso, ecocardiograma
transtorácico, radiografia simples de tórax e contrastadas de esôfago e cólon. O
Holter foi realizado em 98 pacientes e aplicado escores de risco de acidente
vascular encefálico isquĂŞmico e morte. A forma clĂnica indeterminada foi
detectada em 51,6% (96/186) dos pacientes, a cardĂaca em 32,2% (60/186), a
digestiva em 8,1% (15/186) e a cardiodigestiva em 8,1% (15/186). Dos pacientes
com a forma clĂnica digestiva, 7,0% (13/186) apresentaram diferentes grupos de
megaesĂ´fago (I a IV) e 12,9% (24/186) apresentaram megacĂłlon de grau 1 a 3.
Destes, 29,2% (7/24) com ambos os órgãos afetados. As alterações
eletrocardiográficas foram observadas em 48,9% (91/186) dos pacientes e a
arritmia ventricular complexa presente em 41,8% (41/98). Desses pacientes,
10,2% (19/186) apresentaram cardiomegalia, o aneurisma apical foi diagnosticado
em 10,8% (20/186) e, as alterações da contratilidade miocárdica segmentar do
ventrĂculo esquerdo em 33,9% (63/186). Em 7,5% (14/186) dos pacientes foi
detectada insuficiĂŞncia cardĂaca com classes funcionais que variam de I a IV. A
classificação da insuficiĂŞncia cardĂaca por estádios demonstrou que 36,4%
(24/66), 30,3% (20/66), 15,2% (10/66), 13,6% (9/66) e 4,5% (3/66) dos pacientes
apresentaram estágios A, B1, B2, C e D, respectivamente. O escore de
estratificação de risco de acidente vascular encefálico isquêmico identificou 80,6%
(150/186) dos pacientes em baixo risco, 15,6% (29/186) em moderado e 3,8%
(7/186) em alto risco. O escore de risco de morte foi aplicado em 84 pacientes e
69,0% (58/84) mostraram baixo risco, 25,0% (21/84) intermediário e 6,0% (5/84)
alto risco. Os resultados demonstraram a existĂŞncia das principais formas clĂnicas
da doença de Chagas em diferentes estágios de evolução, inclusive o registro da
forma digestiva isolada; observou-se que um quarto dos pacientes com a forma
indeterminada deveria ser considerado cardiopatas subclĂnicos ou pelo menos,
pacientes de maior potencial evolutivo para outras formas clĂnicas ou fase prĂ©-
clĂnica, e confirmou-se a existĂŞncia de correlação positiva entre o escore de risco de morte e seus principais determinantes, insuficiĂŞncia cardĂaca, morte sĂşbita e
acidente vascular encefálico isquêmico, oferecendo mais um elemento para a
tomada de condutas frente ao chagásico cardiopata
Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
Objective: Trypanosoma cruzi is subdivided into six discrete typing units (DTUs), TcI–TcVI. The precise identification of each can contribute to tracking wild DTUs that invade the domiciliary environment.
Methods: Twenty T. cruzi stocks isolated from 16 chagasic patients, two Panstrongylus lutzi, one Galea spixii, and one Euphractus sexcinctus, from different localities in the State of Rio Grande do Norte, Brazil, were characterized by genotyping the 3′ region of the 24Sα rRNA gene, the mitochondrial cytochrome oxidase subunit 2 gene, and the spliced leader intergenic region.
Results: TcIII was identified in 18.7% (3/16) of patients from different municipalities, as well as in P. lutzi, G. spixii, and E. sexcinctus, indicating the connection between the sylvatic and domestic cycles in this Brazilian semi-arid region. TcI and TcII were also detected, in 37.5% (6/16) and 43.8% (7/16) of patients, respectively. These DTUs were associated with cardiac, digestive, and indeterminate clinical forms, while TcIII was identified only in patients with the indeterminate form.
Conclusions: The occurrence of these DTUs reveals important phylogenetic diversity in T. cruzi isolates from humans. TcIII is reported for the first time in northeastern Brazil. These findings appear to indicate an overlap between the sylvatic and domestic transmission cycles of the parasite in this region
Chagas disease: morbidity profile in an endemic area of Northeastern Brazil
Abstract: INTRODUCTION : This study evaluated the clinical forms and manifestation severities of Chagas disease among serologically reactive individuals from Western Rio Grande do Norte (Northeastern Brazil). METHODS : This cross-sectional study included 186 adults who were evaluated using electrocardiography, echocardiography, chest radiography, and contrast radiography of the esophagus and colon. A clinical-epidemiological questionnaire was also used. RESULTS : The indeterminate, cardiac, digestive, and cardiodigestive clinical forms of Chagas disease were diagnosed in 51.6% (96/186), 32.2% (60/186), 8.1% (15/186) and 8.1% (15/186) of the participants, respectively. Heart failure (functional classes I-IV) was detected in 7.5% (14/186) of the participants, and 36.4% (24/66), 30.3% (20/66), 15.2% (10/66), 13.6% (9/66), and 4.5% (3/66) of the patients were at stage A, B1, B2, C, and D, respectively. Dilated cardiomyopathy and electrocardiographic changes were detected in 10.2% (19/186) and 48.1% (91/186) of the participants, respectively. Apical aneurysm was diagnosed in 10.8% (20/186) of the participants, and other changes in the segmental myocardial contractility of the left ventricle were diagnosed in 33.9% (63/186) of the participants. Megaesophagus (groups I-IV) was observed in 7% (13/186) of the participants, megacolon (grades 1-3) was detected in12.9% (24/186) of the participants, and both organs were affected in 29.2% (7/24) of the megacolon cases. CONCLUSIONS : We detected various clinical forms of Chagas disease (including the digestive form). Our findings indicate that clinical symptoms alone may not be sufficient to exclude or confirm cardiac and/or digestive damage, and the number of patients with symptomatic clinical forms may be underestimated
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients.
Chronic chagasic cardiomyopathy (CCC) is observed in 30% to 50% of the individuals infected by Trypanosoma cruzi and heart failure is the important cause of death among patients in the chronic phase of Chagas disease. Although some studies have elucidated the role of adaptive immune responses involving T and B lymphocytes in cardiac pathogenesis, the role of innate immunity receptors such as Toll-like receptors (TLRs) and Nod-like receptors (NLRs) in CCC pathophysiology has not yet been determined. In this study, we evaluated the association among innate immune receptors (TLR1-9 and nucleotide-binding domain-like receptor protein 3/NLRP3), its adapter molecules (Myd88, TRIF, ASC and caspase-1) and cytokines (IL-1β, IL-6, IL-12, IL-18, IL-23, TNF-α, and IFN-β) with clinical manifestation, digestive and cardiac function in patients with different clinical forms of chronic Chagas disease. The TLR8 mRNA expression levels were enhanced in the peripheral blood mononuclear cells (PBMC) from digestive and cardiodigestive patients compared to indeterminate and cardiac patients. Furthermore, mRNA expression of IFN-β (cytokine produced after TLR8 activation) was higher in digestive and cardiodigestive patients when compared to indeterminate. Moreover, there was a positive correlation between TLR8 and IFN-β mRNA expression with sigmoid and rectum size. Cardiac and cardiodigestive patients presented higher TLR2, IL-12 and TNF-α mRNA expression than indeterminate and digestive patients. Moreover, cardiac patients also expressed higher levels of NLRP3, ASC and IL-1β mRNAs than indeterminate patients. In addition, we showed a negative correlation among TLR2, IL-1β, IL-12 and TNF-α levels with left ventricular ejection fraction, and positive correlation between NLRP3 with cardiothoracic index, and TLR2, IL-1β and IL-12 with left ventricular mass index. Together, our data suggest that high expression of innate immune receptors in cardiac and digestive patients may induce an enhancement of cytokine expression and participate of cardiac and digestive dysfunction
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients
Submitted by Sandra Infurna ([email protected]) on 2019-01-30T10:27:25Z
No. of bitstreams: 1
adelaideV_Motta_etal_IOC_2018.pdf: 4348852 bytes, checksum: 2a6663ea033f9812085adebf92dec5ce (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-01-30T10:46:20Z (GMT) No. of bitstreams: 1
adelaideV_Motta_etal_IOC_2018.pdf: 4348852 bytes, checksum: 2a6663ea033f9812085adebf92dec5ce (MD5)Made available in DSpace on 2019-01-30T10:46:20Z (GMT). No. of bitstreams: 1
adelaideV_Motta_etal_IOC_2018.pdf: 4348852 bytes, checksum: 2a6663ea033f9812085adebf92dec5ce (MD5)
Previous issue date: 2018Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil / Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil / Universidade Potiguar. Escola de SaĂşde. Natal, RN, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade do Estado do Rio Grande do Norte. Departamento de CiĂŞncias BiomĂ©dicas. MossorĂł, RN, Brasil.Instituto Internacional de NeurociĂŞncias Edmond e Lilly Safra. MacaĂba, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. LaboratĂłrio de Ultraestrutura Celular. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Análises ClĂnicas e ToxicolĂłgicas. Natal, RN, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasitologia. Natal, RN, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilChronic chagasic cardiomyopathy (CCC) is observed in 30% to 50% of the individuals
infected by Trypanosoma cruzi and heart failure is the important cause of death among
patients in the chronic phase of Chagas disease. Although some studies have elucidated
the role of adaptive immune responses involving T and B lymphocytes in cardiac pathogenesis,
the role of innate immunity receptors such as Toll-like receptors (TLRs) and Nod-like
receptors (NLRs) in CCC pathophysiology has not yet been determined. In this study, we
evaluated the association among innate immune receptors (TLR1-9 and nucleotide-binding
domain-like receptor protein 3/NLRP3), its adapter molecules (Myd88, TRIF, ASC and caspase-
1) and cytokines (IL-1β, IL-6, IL-12, IL-18, IL-23, TNF-α, and IFN-β) with clinical manifestation,
digestive and cardiac function in patients with different clinical forms of chronic
Chagas disease. The TLR8 mRNA expression levels were enhanced in the peripheral blood
mononuclear cells (PBMC) from digestive and cardiodigestive patients compared to indeterminate
and cardiac patients. Furthermore, mRNA expression of IFN-β (cytokine produced
after TLR8 activation) was higher in digestive and cardiodigestive patients when compared
to indeterminate. Moreover, there was a positive correlation between TLR8 and IFN-β
mRNA expression with sigmoid and rectum size. Cardiac and cardiodigestive patients presented
higher TLR2, IL-12 and TNF-α mRNA expression than indeterminate and digestive
patients. Moreover, cardiac patients also expressed higher levels of NLRP3, ASC and IL-1β
mRNAs than indeterminate patients. In addition, we showed a negative correlation among
TLR2, IL-1β, IL-12 and TNF-α levels with left ventricular ejection fraction, and positive correlation
between NLRP3 with cardiothoracic index, and TLR2, IL-1β and IL-12 with left ventricular
mass index. Together, our data suggest that high expression of innate immune.receptors in cardiac and digestive patients may induce an enhancement of cytokine expression
and participate of cardiac and digestive dysfunction
Inflammation Enhances the Risks of Stroke and Death in Chronic Chagas Disease Patients
Submitted by sandra infurna ([email protected]) on 2016-06-19T18:59:10Z
No. of bitstreams: 1
mariaadelaide_matta_etal_IOC_2016.pdf: 749988 bytes, checksum: 989d8777a371bcd276efecde9a5d4214 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-19T19:21:52Z (GMT) No. of bitstreams: 1
mariaadelaide_matta_etal_IOC_2016.pdf: 749988 bytes, checksum: 989d8777a371bcd276efecde9a5d4214 (MD5)Made available in DSpace on 2016-06-19T19:21:52Z (GMT). No. of bitstreams: 1
mariaadelaide_matta_etal_IOC_2016.pdf: 749988 bytes, checksum: 989d8777a371bcd276efecde9a5d4214 (MD5)
Previous issue date: 2016Made available in DSpace on 2016-07-08T12:21:49Z (GMT). No. of bitstreams: 3
mariaadelaide_matta_etal_IOC_2016.pdf.txt: 58546 bytes, checksum: d1a8f1e931ab9591fce414aa980fdaa1 (MD5)
mariaadelaide_matta_etal_IOC_2016.pdf: 749988 bytes, checksum: 989d8777a371bcd276efecde9a5d4214 (MD5)
license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)
Previous issue date: 2016Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasiologia. Natal, RN, BrasilUniversidade do Estado do Rio Grande do Norte. Departamento de CiĂŞncias BiomĂ©dicas. MossorĂł, RN, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasiologia. Natal, RN, Brasil / Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal do Rio Grande do Norte. Departamento de Microbiologia e Parasiologia. Natal, RN, BrasilUniversidade Federal de Ouro Preto. Escola de Medicina. Ouro Preto, MG, Brasil.Univrsidade de SĂŁo Paulo. Escola de Medicina de RibeirĂŁo Preto. RibeirĂŁo Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. LaboratĂłrio deUltraestrutura Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Norte. Departmaneto de Análises ClĂnicas e ToxicolĂłgicas. Natal, RN, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Ischemic strokes have been implicated as a cause of death in Chagas disease patients.
Inflammation has been recognized as a key component in all ischemic processes, including
the intravascular events triggered by vessel interruption, brain damage and repair. In this
study, we evaluated the association between inflammatory markers and the death risk (DR)
and stroke risk (SR) of patients with different clinical forms of chronic Chagas disease. The
mRNA expression levels of cytokines, transcription factors expressed in the adaptive immune
response (Th1, Th2, Th9, Th17, Th22 and regulatory T cell), and iNOS were analyzed by realtime
PCR in peripheral blood mononuclear cells of chagasic patients who exhibited the indeterminate,
cardiac, digestive and cardiodigestive clinical forms of the disease, and the levels of
these transcripts were correlated with the DR and SR. Cardiac patients exhibited lowermRNA
expression levels of GATA-3, FoxP3, AHR, IL-4, IL-9, IL-10 and IL-22 but exhibited higher
expression of IFN-γ and TNF-α compared with indeterminate patients. Digestive patients
showed similar levels of GATA-3, IL-4 and IL-10 than indeterminate patients. Cardiodigestive
patients exhibited higher levels of TNF-α compared with indeterminate and digestive patients.
Furthermore, we demonstrated that patients with high DR and SR exhibited lower GATA-3,
FoxP3, and IL-10 expression and higher IFN-γ, TNF-α and iNOS mRNA expression than
patients with low DR and SR. A negative correlation was observed between Foxp3 and IL-10
mRNA expression and the DR and SR. Moreover, TNF-α and iNOS expression was positively
correlated with DR and SR. Our data suggest that an inflammatory imbalance in chronic Chagas
disease patients is associated with a high DR and SR. This study provides a better understanding
of the stroke pathobiology in the general population and might aid the development of
therapeutic strategies for controlling the morbidity and mortality of Chagas disease
Naturally Leishmania infantum-infected dogs display an overall impairment of chemokine and chemokine receptor expression during visceral leishmaniasis.
Dogs are the primary reservoir for Leishmania parasites. The immune response induced by Leishmania infantum infection in these animals has not been completely elucidated, and few studies have investigated the relationship between the expression levels of chemokines and chemokine receptors and the clinical status of dogs with canine visceral leishmaniasis (CVL). The aim of this study was to correlate the clinical status of naturally L. infantuminfected dogs (from rural areas of MossorĂł city, State of Rio Grande do Norte, Brazil) with the expression levels of chemokines (ccl1, ccl2, ccl3, ccl4, ccl5, ccl17, ccl20, ccl24, ccl26, cxcl9, cxcl10) and chemokine receptors (cxcr3, ccr3, ccr4, ccr5, ccr6, ccr8) in the liver and spleen determined using real-time PCR. Twenty-one dogs were clinically evaluated and classified as asymptomatic (n = 11) or symptomatic (n = 10). Splenomegaly, weight loss and onychogryphosis were the most pronounced symptoms. In the liver, the mRNA expression levels of ccl1, ccl17, ccl26, ccr3, ccr4, ccr5, ccr6, and ccr8 were lower in symptomatic animals than in asymptomatic animals. Compared with uninfected animals, symptomatic dogs had lower expression levels of almost all molecules analyzed. Moreover, high clinical scores were negatively correlated with ccr5 and ccr6 expression and positively correlated with cxcl10 expression. We conclude that the impairment of the expression of chemokines and chemokine receptors results in deficient leukocyte migration and hampers the immune response, leading to the development of disease