Adipose Tissue Epigenetic Profile in Obesity-Related Dysglycemia - A Systematic Review.

BackgroundObesity is a major risk factor for dysglycemic disorders, including type 2 diabetes (T2D). However, there is wide phenotypic variation in metabolic profiles. Tissue-specific epigenetic modifications could be partially accountable for the observed phenotypic variability.ScopeThe aim of this systematic review was to summarize the available data on epigenetic signatures in human adipose tissue (AT) that characterize overweight or obesity-related insulin resistance (IR) and dysglycemia states and to identify potential underlying mechanisms through the use of unbiased bioinformatics approaches.MethodsOriginal data published in the last decade concerning the comparison of epigenetic marks in human AT of individuals with metabolically unhealthy overweight/obesity (MUHO) versus normal weight individuals or individuals with metabolically healthy overweight/obesity (MHO) was assessed. Furthermore, association of these epigenetic marks with IR/dysglycemic traits, including T2D, was compiled.ResultsWe catalogued more than two thousand differentially methylated regions (DMRs; above the cut-off of 5%) in the AT of individuals with MUHO compared to individuals with MHO. These DNA methylation changes were less likely to occur around the promoter regions and were enriched at loci implicated in intracellular signaling (signal transduction mediated by small GTPases, ERK1/2 signaling and intracellular trafficking). We also identified a network of seven transcription factors that may play an important role in targeting DNA methylation changes to specific genes in the AT of subjects with MUHO, contributing to the pathogeny of obesity-related IR/T2D. Furthermore, we found differentially methylated CpG sites at 8 genes that were present in AT and whole blood, suggesting that DMRs in whole blood could be potentially used as accessible biomarkers of MUHO.ConclusionsThe overall evidence linking epigenetic alterations in key tissues such AT to metabolic complications in human obesity is still very limited, highlighting the need for further studies, particularly those focusing on epigenetic marks other than DNA methylation. Our initial analysis suggests that DNA methylation patterns can potentially discriminate between MUHO from MHO and provide new clues into why some people with obesity are less susceptible to dysglycemia. Identifying AT-specific epigenetic targets could also lead to novel approaches to modify the progression of individuals with obesity towards metabolic disease.Systematic review registrationPROSPERO, identifier CRD42021227237

Immunohistochemical approach to the pathogenesis of clinical cases of Bovine Herpesvirus type 5 infections

Meningoencephalitis by Herpesvirus type 5 (BoHV-5) in cattle has some features that are similar to those of herpetic encephalitis in humans and other animal species. Human Herpesvirus 3 (commonly known as Varicella-zoster virus 1), herpes simplex viruses (HSV), and equid Herpesvirus 1 (EHV-1) induce an intense inflammatory, vascular and cellular response. In spite of the many reports describing the histological lesions associated with natural and experimental infections, the immunopathological mechanisms for the development of neurological disorder have not been established. A total of twenty calf brains were selected from the Veterinary School, University of São Paulo State, Araçatuba, Brazil, after confirmation of BoHV-5 infection by virus isolation as well as by a molecular approach. The first part of the study characterized the microscopic lesions associated with the brain areas in the central nervous system (CNS) that tested positive in a viral US9 gene hybridization assay. The frontal cortex (Fc), parietal cortex (Pc), thalamus (T) and mesencephalon (M) were studied. Secondly, distinct pathogenesis mechanisms that take place in acute cases were investigated by an immunohistochemistry assay. This study found the frontal cortex to be the main region where intense oxidative stress phenomena (AOP-1) and synaptic protein expression (SNAP-25) were closely related to inflammatory cuffs, satellitosis and gliosis, which represent the most frequently observed neurological lesions. Moreover, MMP-9 expression was shown to be localized in the leptomeninges, in the parenchyma and around mononuclear infiltrates (p < 0.0001). These data open a new perspective in understanding the role of the AOP-1, MMP-9 and SNAP-25 proteins in mediating BoHV-5 pathogenesis and the strategies of host-virus interaction in order to invade de CNS

Efeitos da adubação orgânica e da época de colheita na qualidade da matéria-prima e nos rendimentos agrícola e de açúcar mascavo artesanal de duas cultivares de cana-de-açúcar (cana-planta).

Conduziu-se este trabalho com o objetivo de estudar os efeitos de três sistemas de adubação (30 t. ha-1 de esterco de curral, 3,5 t.ha-1 de esterco de galinha e adubação química - 120 kg.ha-1 de P2O5 e de K2O no plantio + 60 kg.ha-1 de N em cobertura) e três épocas de colheita da cana (julho, agosto e setembro de 2003), na qualidade da matéria-prima e nos rendimentos de colmos e de açúcar mascavo de duas cultivares de cana-de-açúcar (SP79-1011 e RB72454). O experimento foi instalado em área do Alambique JM, Perdões, MG. O delineamento experimental foi o de blocos casualizados, em esquema fatorial (2 x 3 x 3), com três repetições. Não houve efeito dos fertilizantes nos rendimentos de colmos e de açúcar mascavo das cultivares estudadas. Verificou-se efeito de épocas de colheita no rendimento de colmos, com destaque para os meses de agosto e setembro. No entanto, para rendimento de açúcar mascavo nenhuma diferença foi observada. Assim, nas condições deste trabalho, é viável a substituição da adubação química pela orgânica (esterco de curral ou de galinha), sem perdas na qualidade da matéria-prima e nos rendimentos de colmos e de açúcar mascavo artesanal, sendo que os meses de agosto e setembro foram os que proporcionaram matéria-prima de melhor qualidade e maiores rendimentos de colmos

Influence of migration on the thought process of individuals at ultra-high risk for psychosis

OBJECTIVE To assess the influence of migration on the psychopathological presentation of individuals at ultra-high risk for psychosis (UHR) in São Paulo, Brazil. METHODS This study is part of the Subclinical Symptoms and Prodromal Psychosis (SSAPP) project, a cohort study in São Paulo, Brazil, designed to follow individuals at UHR. After screening with the Prodromal Questionnaire (PQ) and a clinical interview, the Global Assessment of Functioning (GAF) was administered, a neuropsychological assessment was performed, sociodemographic and migration data were obtained. We then analyzed UHR individuals who had migration data to see if migration had any effect on their cognition and psychopathology. Chi-square tests were used for categorical variables, and Student's t test or analysis of variance (ANOVA) were used for nonparametric and parametric distributions, respectively. RESULTS The sample was composed of 42 at-risk subjects, of whom 5 had a migration history in the past two generations. Those with migration history showed significantly more formal thought disturbances (p = 0.012) and sleeping problems (p = 0.033) compared to those without. CONCLUSIONS Our data reinforce migration as a risk factor for psychosis in developing countries as well, and highlights the importance of studying the specific effect of this factor in UHR psychopathology

Relationship between Field Measurements in Three \u3cem\u3eBrachiaria\u3c/em\u3e Species with Leaf Area Index and Light Interception by Indirect Methods

Brachiaria species play a strategic role in ruminant production systems in Brazil, covering an estimated pasture area of approximately 90 million hectares (Karia et al., 2006), however, these pastures are subject to different degrees of degradation due to inadequate management. In pasture management, field measurements such as canopy height, for example, are used by managers as a tool to establish parameters for the optimal point to cut the forage and for the post-grazing residue, in order to maximize production by harvesting at maximum of herbage mass accumulation, and to avoid problems associated to overgrazing, by setting ideal post-grazing height for forage regrowth. The use of the variables light interception (LI) and leaf area index (LAI) has been recommended as a tool for pasture management, based on the theory that, when the canopy reaches a light interception of 95%, the forage is near its maximum growth rate, which is called critical LAI (Brougham, 1956). The residual LAI refers to the leaf area of the post-grazed stubble. Residual LAI is used to establish the minimum leaf area necessary to ensure an efficient pasture regrowth (Lemos et al., 2014). Light interception and the LAI are difficult to measure at the farm level, due to the high cost of the equipment and technical feasibility of the process. The objective of this study was to evaluate the relationship between LI and LAI measured by two different equipment, with canopy height and soil cover in three species of Brachiaria

\u3cem\u3eIn Situ\u3c/em\u3e Digestibility of \u3cem\u3eGliricidia sepium\u3c/em\u3e Combined with \u3cem\u3eBrachiaria decumbens\u3c/em\u3e in a Silvopastoral System

Silvopastoral system (SPS) are characterized by a combination of trees, pasture and herbivores animals, in the same physical area, in order to obtain diversified products. A promising legume tree that has been studied and used in SPS in tropical areas is gliricidia [Gliricidia sepium (Jacq.) Steud]. Advantages of gliricidia use in SPS include N inputs via biological fixation, improvement of soil properties, nutrient cycling and also a source of feed to grazing animals (Cubillos-Hinojosa et al., 2011). Gliricidia has high crude protein concentration in its leaves, which complements the usual N-poor diet of ruminants grazing warm-season grasses. The introduction of gliricidia in SPS faces a problem due to the low initial acceptability by cattle, being necessary an adaptation period in order to cattle reach satisfactory intake levels (Carvalho Filho et al., 1997). In general, the quality of the forages can be predicted by accessing their nutritive value, represented by the chemical composition and digestibility of the forage constituents (Van Soest, 1994). The digestibility of dry matter in forages consumed in a SPS can be influenced by the forage species used, by grass/legume combinations, and by the proportion that each forage species takes in the diet of the ruminants. This study evaluated in situ digestibility of gliricidia in increasing levels of inclusion in the diet composed by sabi grass (Brachiaria decumbens, Stapf) in a silvopastoral system

Management Effect on Rainfall Use Efficiency and Growth Rate of Spineless Cactus \u3cem\u3ecv.\u3c/em\u3e Miúda in Northeast Brazil

Spineless cactus has a high rainfall use efficiency. Under water deficit, they open their stomata during the night to capture atmospheric carbon dioxide when the air temperature is lower and relative humidity higher (Taiz and Zeiger, 2009), losing less water to the atmosphere. The rainfall use efficiency (RUE) can be expressed as the amount of water used by the crop to produce dry matter (Han and Felker, 1997). The RUE showed by spineless cactus, a CAM plant, is three to five times greater than the one observed in C3 and C4 plants (Felker and Russel, 1988; Nobel, 1991). Therefore, spineless cacti (Nopaleae opuntia) are important forage resource in northeast Brazil. Management practices, however, can affect the RUE and growth of spineless cactus, especially organic and mineral fertilization. Thus, the objective of this research was to evaluate the effect of organic and nitrogen fertilization and harvest frequency on rainfall use efficiency and growth rate of spineless cactus cv. Miúda (Nopalea cochenillifera Salm Dyck)

Agricultural intensification can help protect the Amazon Forest and reduce global warming / Protecting the Amazon forest and reducing global warming via agricultural intensification

The Amazon basin includes 550 M ha covered with rainforests, with 60% of this area being in Brazil. Conversion of rainforest for soybean production raises concerns about the degree to which Brazil can reconcile production and environmental goals. Here we investigated the degree to which intensification could help Brazil produce more soybean without further encroachment of the Amazon Forest. Our analysis shows that continuation of current trends in soybean yield and area would lead to conversion of additional 5.7 M ha of forests and savannas during the next 15 years, with an associated 2550 Mt of CO2eq released into the atmosphere. In contrast, acceleration of yield improvement, coupled with expansion of soybean area only in areas currently used for livestock production, would allow Brazil to achieve similar economic benefits without deforestation and with substantially lower global climate warming

Acute febrile illness is associated with Rickettsia spp infection in dogs

BACKGROUND: Rickettsia conorii is transmitted by Rhipicephalus sanguineus ticks and causes Mediterranean Spotted Fever (MSF) in humans. Although dogs are considered the natural host of the vector, the clinical and epidemiological significance of R. conorii infection in dogs remains unclear. The aim of this prospective study was to investigate whether Rickettsia infection causes febrile illness in dogs living in areas endemic for human MSF. METHODS: Dogs from southern Italy with acute fever (n = 99) were compared with case–control dogs with normal body temperatures (n = 72). Serology and real-time PCR were performed for Rickettsia spp., Ehrlichia canis, Anaplasma phagocytophilum/A. platys and Leishmania infantum. Conventional PCR was performed for Babesia spp. and Hepatozoon spp. Acute and convalescent antibodies to R. conorii, E. canis and A. phagocytophilum were determined. RESULTS: The seroprevalence rates at first visit for R. conorii, E. canis, A. phagocytophilum and L. infantum were 44.8%, 48.5%, 37.8% and 17.6%, respectively. The seroconversion rates for R. conorii, E. canis and A. phagocytophilum were 20.7%, 14.3% and 8.8%, respectively. The molecular positive rates at first visit for Rickettsia spp., E. canis, A. phagocytophilum, A. platys, L. infantum, Babesia spp. and Hepatozoon spp. were 1.8%, 4.1%, 0%, 2.3%, 11.1%, 2.3% and 0.6%, respectively. Positive PCR for E. canis (7%), Rickettsia spp. (3%), Babesia spp. (4.0%) and Hepatozoon spp. (1.0%) were found only in febrile dogs. The DNA sequences obtained from Rickettsia and Babesia PCRs positive samples were 100% identical to the R. conorii and Babesia vogeli sequences in GenBank®, respectively. Febrile illness was statistically associated with acute and convalescent positive R. conorii antibodies, seroconversion to R. conorii, E. canis positive PCR, and positivity to any tick pathogen PCRs. Fourteen febrile dogs (31.8%) were diagnosed with Rickettsia spp. infection based on seroconversion and/or PCR while only six afebrile dogs (12.5%) seroconverted (P = 0.0248). The most common clinical findings of dogs with Rickettsia infection diagnosed by seroconversion and/or PCR were fever, myalgia, lameness, elevation of C-reactive protein, thrombocytopenia and hypoalbuminemia. CONCLUSIONS: This study demonstrates acute febrile illness associated with Rickettsia infection in dogs living in endemic areas of human MSF based on seroconversion alone or in combination with PCR

Pernambuco Semiarid Native Rhizobial Populations Nitrogen Fixation Potential with Native \u3cem\u3eMacroptilium\u3c/em\u3e

Nitrogen (N) is one of nature´s most abundant elements, accounting for about 78% of the atmospheric gases, but mostly as the inert N2 form. As such it is not directly available to plants, and is relatively scarce in most agroecosystems. Biological nitrogen fixation (BNF) through diazotrophic bacteria represents ca. 63% of the yearly N input in terrestrial ecosystems (Taiz and Zeiger 2004). Legumes which form effective symbiosis with the diazotrophic group of bacteria commonly known as rhizobia, are a very important source of available N. Tropical forage legumes are usually able to nodulate with a diverse population of rhizobia, and may have a relevant contribution to nitrogen availability in pastures (Santos et al. 2003). This diversity may be exploited to find more symbiotically efficient bacterial strains, thereby increasing legume effects on pastures. One way to evaluate this diversity is to isolate strains from different regions, vegetation covers or cultivation systems, and environmental conditions. This practice would potentially lead to a large number of isolates, which would increase the chance of finding some more efficient than those currently available (Chagas Junior et al, 2010). Native legumes, including several species of Macroptilium are an important forage resource in the Brazilian Northeast semiarid, contributing to the quality of ruminant diet, but they are still not well known in regards to their BNF ability. This work evaluated nodulation efficiency of Macroptilium lathyroides when inoculated with Litolic Neossol from eight municipalities of Pernambuco State semiarid