USO DE SUCEDÂNEO EM SUBSTITUIÇÃO AO LEITE NO DESEMPENHO DE BEZERROS DA RAÇA HOLANDESA DURANTE A CRIA E RECRIA

O objetivo deste trabalho foi avaliar a eficiência biológica do sucedâneo em substituição ao leite,na fase de cria e recria de bezerros da raça Holandesa. O trabalho foi realizado na UniversidadeTecnológica Federal do Paraná (UTFPR), Dois Vizinhos, entre maio de 2011 e abril de 2012. Apesquisa foi realizada com 32 animais, adotando-se o delineamento inteiramente casualizado comquatro tratamentos e oito repetições: 100% leite; 34% sucedâneo + 66% leite; 66% sucedâneo + 34%leite; e 100% sucedâneo. Os dados foram submetidos à analise de variância e de regressão a 5% designificância. Dentre as medidas morfométricas realizadas, apenas o ganho de perímetro torácicoapresentou diferença significativa. A utilização de sucedâneo em substituição ao leite durante a fase dealeitamento apresentou uma resposta linear negativa para os níveis 100%, 66%, 34% sucedâneo, porémdurante a fase de recria ocorreu um ganho compensatório dos animais submetidos aos tratamentos34% e 66% sucedâneo. A utilização de 100% de sucedâneo na alimentação de bezerros holandeses nafase de cria não foi eficiente durante o aleitamento. Durante a fase de recria, a substituição parcial doleite por sucedâneo (66% sucedâneo + 34% leite) mostrou ser a opção mais eficiente, pois apresentouum ganho compensatório favorável. Palavras-chave: consumo; macho leiteiro; medidas morfométricas

MILK REPLACER USE IN SUBSTITUTION OF WHOLE MILK ON THE PERFORMANCE OF HOLSTEIN CALVES DURING GROWING PHASE

The objective of this study was to evaluate the biological efficiency of the milk replacer in the substitution of whole milk for calves at the first months of age and at rearing fase. This study was conducted in Universidade Tecnológica Federal do Paraná (UTFPR), Campus Dois Vizinhos, from May 2011 to April 2012. This research was carried out on 32 animals, assigned in a completely randomized design with four treatments (100% whole milk, 34% milk replacer + 66% whole milk, 66% milk replacer + 34% whole milk and 100% milk replacer) and eight replications.The data were analyzed by analysis of variance and regression at 5% of significance. Regarding the morphometric measurements, only the gain of chest circumference presented significant difference. The use of milk replacer in the substitution of whole milk during pre-weaning phase showed negative linear response for levels 100%, 66%, 34% milk replacer, nevertheless during the post-weaning phase there was a compensatory gain for the animals fed with 34% and 66 % milk replacer. The use of 100% milkreplacer for Holstein calves at pre-weaning phase was not efficient during lactation, althoug, during post-weaning phase, partial replacement of milk by milk replacer (66% + 34% milk replacer) was an efficient option because there was a favorable compensatory growth

Impairment of Multiple Mitochondrial Energy Metabolism Pathways in the Heart of Chagas Disease Cardiomyopathy Patients

International audienceChagas disease cardiomyopathy (CCC) is an inflammatory dilated cardiomyopathy occurring in 30% of the 6 million infected with the protozoan Trypanosoma cruzi in Latin America. Survival is significantly lower in CCC than ischemic (IC) and idiopathic dilated cardiomyopathy (DCM). Previous studies disclosed a selective decrease in mitochondrial ATP synthase alpha expression and creatine kinase activity in CCC myocardium as compared to IDC and IC, as well as decreased in vivo myocardial ATP production. Aiming to identify additional constraints in energy metabolism specific to CCC, we performed a proteomic study in myocardial tissue samples from CCC, IC and DCM obtained at transplantation, in comparison with control myocardial tissue samples from organ donors. Left ventricle free wall myocardial samples were subject to two-dimensional electrophoresis with fluorescent labeling (2D-DIGE) and protein identification by mass spectrometry. We found altered expression of proteins related to mitochondrial energy metabolism, cardiac remodeling, and oxidative stress in the 3 patient groups. Pathways analysis of proteins differentially expressed in CCC disclosed mitochondrial dysfunction, fatty acid metabolism and transmembrane potential of mitochondria. CCC patients’ myocardium displayed reduced expression of 22 mitochondrial proteins belonging to energy metabolism pathways, as compared to 17 in DCM and 3 in IC. Significantly, 6 beta-oxidation enzymes were reduced in CCC, while only 2 of them were down-regulated in DCM and 1 in IC. We also observed that the cytokine IFN-gamma, previously described with increased levels in CCC, reduces mitochondrial membrane potential in cardiomyocytes. Results suggest a major reduction of mitochondrial energy metabolism and mitochondrial dysfunction in CCC myocardium which may be in part linked to IFN-gamma. This may partially explain the worse prognosis of CCC as compared to DCM or IC

<i>In silico</i> analysis predicted differential binding patterns for rs640249 and rs641563 polymorphisms. Gel shift experiment has confirmed this differential binding for rs640249 polymorphism.

<p>The probability of these polymorphisms creating or altering DNA–protein interaction was determined by in silico analysis (<a href="http://www.gene-regulation.com/cgi-bin/pub/programs/match/bin/match.cgi" target="_blank"><u>http://www.gene-regulation.com/cgi-bin/pub/programs/match/bin/match.cgi</u></a>). An 75% threshold score (similarity matrix) was used. For each polymorphism (A, rs1800925; B, rs641563) putative bindings are described. The similarity matrix score is indicated in brackets. <b>C</b>: Electrophoretic mobility shift assays were performed in vitro as described in Materials and Methods. A differential binding pattern was detected for the rs640249A allele. </p

Correlation analysis for the rs640249 polymorphism in two different reference populations.

<p>Genotype data from the European reference population (CEU) and from the West Africa reference population (YRI) were downloaded from HapMap. A 2 Mb region surrounding the ACTC1 gene was analyzed in these two populations. The data were analyzed with Haploview Software. <b>A)</b> Correlations were assessed by calculating r² values. The rs640249 polymorphism was found to be correlated with three other polymorphisms in the CEU reference population (rs641563; rs639735; rs479623). <b>B</b>) In the West Africa reference population, the rs640249 polymorphism was correlated only with rs641563. </p

Relative quantification of alpha-cardiac actin 1 (ACTC1) by immunoblotting.

<div><p>Myocardial samples were obtained from the left ventricular free wall of the hearts of patients with severe CCC and end-stage heart failure, at the time of heart transplantation. Samples from five hearts from CCC patients (at least two positive results in three independent anti-<i>T</i>. <i>cruzi</i> serology tests, as indicated above), and from healthy hearts from organ donors not used for transplantation for technical reasons were used. Immunoblotting and protein quantification were done in duplicate. A. The immunoblot and the protein quantification result of the first experiment are presented here. The central line represents the median. Representative results from two experiments are shown here. A Mann-Whitney test was performed and differences were considered significant if <i>P</i><0.001.</p> <p><b>B</b>. Real-time quantitative PCR was carried out on the same samples. All the samples were tested in triplicate with GAPDH, the expression of which has been shown to vary little between human myocardial tissue samples. Data were normalized and the relative levels of each mRNA were calculated by the 2^-ΔCt method.</p></div

The same trends for association were detected in a small, independent replication cohort.

<p>The two main polymorphisms, rs640249 and rs641563, were genotyped in the independent replication cohort. This second cohort focused exclusively on male patients with Chagas disease, who have a higher risk of progression to CCC. This replication cohort included asymptomatic (<i>n</i> = 36) and CCC patients (<i>n</i> = 102). Of the 106 patients with CCC, 48 had severe ventricular dysfunction (left ventricular ejection fraction <40%). The rs640249 genotype distribution is illustrated in A. The results for the rs641563 polymorphism are in B. Haplotype analysis was then performed in C.</p

The rs640249A-rs641563A haplotype is associated with resistance to CCC.

<p>Based on the genotypes obtained, we performed a haplotype analysis of the rs7719175 and rs1800925 polymorphisms. Only three haplotypes were found in our study population: <b>A</b>) Distribution of the three main haplotype combinations in the CCC patients and ASY subjects: homozygous rs640249C-rs641563C (black bar); heterozygous rs640249C-rs641563C + rs640249A-rs641563A (gray bar) and homozygous rs640249A-rs641563A (white bar). <b>B</b>) Haplotype combinations between cases and controls, taking sex into account. <b>C</b>) Distribution of the three main haplotype combinations between patients with severe and moderate CCC, taking sex into account.</p