Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials

The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods: The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results: Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions: These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic

Development and characterization of a novel bulk-fill elastomeric temporary restorative composite

Objectives: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). Material and Methods: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans' biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). Results: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. Conclusions: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values

Correlation between bisphosphonates and users of muco-supported prostheses and osteonecrosis of the jaws - systematic review

In this research, a systematic review of papers dealing with the relationship between osteonecrosis in patients using bisphosphonates and local predisposition factors was performed. It aims to determine the relationship between users of bisphosphonates in muco-supported and / or dento-muco-supported prostheses and incidence of osteonecrosis. A detailed search in four databases was performed: MedLine (PubMed), Web of Science, Scopus and Cochrane, by two independent reviewers from June to November 2018, using the search strategy developed by PubMed (MedLine) and adapted to other databases. A total of 1352 potentially relevant articles were identified in all databases. Deleting the duplicates, 740 articles left, of which, after title and abstract analysis 691 studies were excluded because they did not meet the eligibility criteria. From the 49 studies maintained for the detailed review, 37 studies were excluded because they did not present a relationship between the use of bisphosphonates with prosthesis users. A total of 12 studies fulfilled all the selection criteria and were included in the systematic review. Although the PRISMA criteria work with the idea of randomized clinical trials, and that prospective and retrospective studies should be included, only two articles did not fit this classification. According to the findings, we can conclude that bisphosphonate users who use dento-mucous or muco-supported prostheses are susceptible to osteonecrosis lesions due to trauma caused by the use of prostheses, requiring the use of hygiene protocols and the choice of materials for the preparation of more malleable prostheses and which prevent or minimize trauma to the adjacent mucosa.Sem bolsaNesta pesquisa, foi realizada uma revisão sistemática de trabalhos que tratam da relação entre osteonecrose em pacientes usuários de bisfosfonatos e os fatores de predisposição local. Tem o objetivo de determinar a relação entre usuários de bisfosfonatos e que são portadores de próteses muco-suportadas e/ou dento-muco-suportadas e incidência de osteonecrose. Uma busca detalhada em quatro bases de dados foi realizada: MedLine (PubMed), Web of Science, Scopus e Cochrane, por dois revisores independentes, no período de junho a novembro de 2018, utilizando a estratégia de busca desenvolvida pelo PubMed (MedLine) e adaptado para outras bases de dados. Um total de 1352 artigos potencialmente relevantes foram identificados em todas as bases de dados. Retirando as duplicatas restaram 740 artigos, dos quais, após análise do título e resumo 691 estudos foram excluídos por não atenderem aos critérios de elegibilidade. Dos 49 estudos mantidos para a revisão detalhada, 37 estudos foram excluídos por não apresentarem relação do uso de bisfosfonatos com usuários de próteses. Um total de 12 estudos preencheram todos os critérios de seleção e foram incluídos na revisão sistemática. Embora os critérios PRISMA trabalham com a ideia de estudos clínicos randomizados, e que estudos prospectivos e retrospectivos devam ser incluídos, apenas dois artigos não se enquadraram nessa classificação. Conforme os achados podemos concluir que usuários de bisfosfonatos e que utilizam próteses dentomuco ou muco-suportadas são suscetíveis a lesões de osteonecrose em virtude de trauma causada pelo uso de próteses, sendo necessário utilização de protocolos de higienização e escolha de materiais para confecção de próteses mais maleáveis e que evitem ou minimizem o trauma na mucosa adjacente

Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials

Abstract The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic

Development and characterization of a novel bulk-fill elastomeric temporary restorative composite

Abstract Objectives: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). Material and Methods: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans’ biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). Results: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. Conclusions: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values