Comparing the Accuracy of Egg Candling and Egg Flotation to Estimate the Hatching Date of Northern Bobwhite Clutches

Floating and candling avian eggs to assess hatch dates has been used successfully to estimate hatch dates for wild bird clutches for decades. However, there is a dearth of information assessing the accuracy of these techniques to estimate northern bobwhite (Colinus virginianus) hatch dates. We captured and fitted a hen bobwhites with very high frequency transmitters during January and February of 2011–2012. We monitored each bird twice weekly until nesting was initiated. We searched for the nest while the hen was away from the nest (i.e., feeding) to reduce potential abandonment. We used egg floatation and egg candling methods to attempt to estimate wild northern bobwhite clutches during the 2011–2012 nesting seasons. We used a mini MagLite© (97 lumens; Mag Instrument, Inc., Ontario, CA, USA) with the glass lens removed so eggs would sit near the bulb to increase the illumination. We used a dark green 68-cm x 137-cm towel to cover the observer in the field to reduce the naturally occurring light, which might have reduced the visibility of the chick embryo. We based age of the eggs (no. of days since the start of incubation) on the embryo growth stage at the time of nest discovery. We conducted egg flotation at the same time as candling. We used a 100-mL glass beaker with 100-mL of ambient temperature tap water to completely submerge the egg to estimate hatch date. We based the flotation estimation age on the angle at which the egg floated in the water. We also conducted a controlled laboratory experiment using pen-raised quail eggs collected from the breeding colony at the Quail-Tech Alliance breeding facility in Lubbock, Texas. We placed 110 eggs in a commercial incubator that was maintained at 37° C with 55% humidity for the duration of the study. We used 3 novice observers to determine the impact of observer bias on the techniques of estimating hatch date. We placed random groups of eggs (i.e., 5–15 eggs at a time until 110 eggs were obtained) into the incubator at a staggered rate to increase variation in the study. We used the same field techniques for hatch date estimation in the controlled study. We first floated eggs during both controlled and field observations to reduce any potential bias that candling might have on the hatch date estimation (i.e., lack of embryo growth). During the controlled study observers examined the eggs individually. Using the average estimated hatch date (Julian date) as a predictor, we used linear regression to determine the accuracy of the candling and floating methods. We also used a linear regression to determine the accuracy of each estimation technique and observers. When candle and egg floating occurred in a field setting, both methods were found to overestimate the actual hatch date of the clutches discovered (n = 47; R2 = 0.993, P \u3c 0.001; estimated hatch days when using candle: x̄ = 1.21 ± 0.92 days, floating: x̄ = 0.89 ± 0.97 days). However, the mean difference between the candling and flotation was -0.38 days (SE = 1.07 days). Regression analysis suggests that candling and egg flotation are fairly accurate predictors of the actual hatch date for newly discovered bobwhite nests (candling: β = 0.43, t = 3.75, P = 0.001; floating: β = 0.53, t = 4.79, P \u3c 0.001). Use of the candling method appears to be correct 43% of the time whereas egg flotation accurately predicted the estimated hatch date 53% of the time. Under controlled conditions, all 3 observers were new to both techniques of hatch date estimation and were all taught by the same instructor for each method. During the controlled test, we found that observers were highly variable. Two observers could predict the estimated hatch date by using the candling and egg flotation methods to a close estimation of the actual hatch date (floating [observer 1: β = 0.23, t = 2.80, P = 0.006 and observer 2: β = 0.47, t = 5.52, P \u3c 0.001]; candling [observer 1: β = 0.30, t = 4.00, P = 0.006, observer 2: β = 0.219, P \u3c 0.01]). Although observer 3 was unable to predict the estimated hatch date for both estimation methods (floating: β = –0.001, t = –0.013, P = 0.684; candling: β = 0.043, t = 0.40, P = 0.990). We also examined any potential abandonment or hatchability issues that might have risen while using candling or floating to estimate hatch dates for wild clutches. We found that 0.06% (5 of 80 nests) of hens abandoned their clutches during this study. Of the 5 nests that were abandoned zero were abandoned because of measurements obtained during the initial investigation of the nest site. All abandonments were due to either weather (i.e., summer hail), predators, or livestock. Viability and hatchability were unaffected for the remaining clutches that were measured during the field study. We found that candling and egg flotation are both viable methods for estimating hatch dates of bobwhite clutches during an initial measurement when a nest is discovered. When an entire clutch is measured accuracy can be within 1 day of the actual estimated hatch date (based on a 24-day incubation period). However, observers or researchers who will estimate hatch dates for clutches should be properly trained and allowed time to acclimate to the measuring techniques to potentially increase their accuracy at estimating hatch dates for northern bobwhite clutches

Missileborne Artificial Vision System (MAVIS)

Several years ago when INTEL and China Lake designed the ETANN chip, analog VLSI appeared to be the only way to do high density neural computing. In the last five years, however, digital parallel processing chips capable of performing neural computation functions have evolved to the point of rough equality with analog chips in system level computational density. The Naval Air Warfare Center, China Lake, has developed a real time, hardware and software system designed to implement and evaluate biologically inspired retinal and cortical models. The hardware is based on the Adaptive Solutions Inc. massively parallel CNAPS system COHO boards. Each COHO board is a standard size 6U VME card featuring 256 fixed point, RISC processors running at 20 MHz in a SIMD configuration. Each COHO board has a companion board built to support a real time VSB interface to an imaging seeker, a NTSC camera, and to other COHO boards. The system is designed to have multiple SIMD machines each performing different corticomorphic functions. The system level software has been developed which allows a high level description of corticomorphic structures to be translated into the native microcode of the CNAPS chips. Corticomorphic structures are those neural structures with a form similar to that of the retina, the lateral geniculate nucleus, or the visual cortex. This real time hardware system is designed to be shrunk into a volume compatible with air launched tactical missiles. Initial versions of the software and hardware have been completed and are in the early stages of integration with a missile seeker

Nest-Site Characteristics of Northern Bobwhites Translocated Into Weeping Lovegrass CRP

Habitat loss and fragmentation have been considered major causes for the decline of northern bobwhite (Colinus virginianus). There are . 400,000 ha of weeping lovegrass (Eragrostis curvula) Conservation Reserve Program (CRP) fields in the Southern High Plains of Texas some of which could be modified to provide usable habitat for northern bobwhites. Timely colonization of improved CRP habitat by northern bobwhite is unlikely without transplantation, because of distance from existing populations. We radio-marked and transplanted 94 northern bobwhite into weeping lovegrass CRP and monitored nest success. We recorded high nest success in 2002 (70%) and 2003 (71%) for northern bobwhite nesting in weeping lovegrass CRP in the area studied. The composition of weeping lovegrass CRP fields available in our study area appears to be suitable nesting cover for northern bobwhite

Social Determinants of Health and Tobacco Use in Thirteen Low and Middle Income Countries: Evidence from Global Adult Tobacco Survey

BACKGROUND: Tobacco use has been identified as the single biggest cause of inequality in morbidity. The objective of this study is to examine the role of social determinants on current tobacco use in thirteen low-and-middle income countries. METHODOLOGY/PRINCIPAL FINDINGS: We used nationally representative data from the Global Adult Tobacco Survey (GATS) conducted during 2008-2010 in 13 low-and-middle income countries: Bangladesh, China, Egypt, India, Mexico, Philippines, Poland, Russian Federation, Thailand, Turkey, Ukraine, Uruguay, and Viet Nam. These surveys provided information on 209,027 respondent's aged 15 years and above and the country datasets were analyzed individually for estimating current tobacco use across various socio-demographic factors (gender, age, place of residence, education, wealth index, and knowledge on harmful effects of smoking). Multiple logistic regression analysis was used to predict the impact of these determinants on current tobacco use status. Current tobacco use was defined as current smoking or use of smokeless tobacco, either daily or occasionally. Former smokers were excluded from the analysis. Adjusted odds ratios for current tobacco use after controlling other cofactors, was significantly higher for males across all countries and for urban areas in eight of the 13 countries. For educational level, the trend was significant in Bangladesh, Egypt, India, Philippines and Thailand demonstrating decreasing prevalence of tobacco use with increasing levels of education. For wealth index, the trend of decreasing prevalence of tobacco use with increasing wealth was significant for Bangladesh, India, Philippines, Thailand, Turkey, Ukraine, Uruguay and Viet Nam. The trend of decreasing prevalence with increasing levels of knowledge on harmful effects of smoking was significant in China, India, Philippines, Poland, Russian Federation, Thailand, Ukraine and Viet Nam. CONCLUSIONS/SIGNIFICANCE: These findings demonstrate a significant but varied role of social determinants on current tobacco use within and across countries

YqiC of Salmonella enterica serovar Typhimurium is a membrane fusogenic protein required for mice colonization

<p>Abstract</p> <p>Background</p> <p><it>Salmonella enterica </it>serovar Typhimurium is an intracellular bacterial pathogen which can colonize a variety of hosts, including human, causing syndromes that vary from gastroenteritis and diarrhea to systemic disease.</p> <p>Results</p> <p>In this work we present structural information as well as insights into the <it>in vivo </it>function of YqiC, a 99-residue protein of <it>S</it>. Typhimurium, which belongs to the cluster of the orthologous group 2960 (COG2960). We found that YqiC shares biophysical and biochemical properties with <it>Brucella abortus </it>BMFP, the only previously characterized member of this group, such as a high alpha helix content, a coiled-coil domain involved in trimerization and a membrane fusogenic activity <it>in vitro</it>. In addition, we demonstrated that YqiC localizes at cytoplasmic and membrane subcellular fractions, that a <it>S</it>. Typhimurium <it>yqiC </it>deficient strain had a severe attenuation in virulence in the murine model when inoculated both orally and intraperitoneally, and was impaired to replicate at physiological and high temperatures <it>in vitro</it>, although it was still able to invade and replicate inside epithelial and macrophages cell lines.</p> <p>Conclusion</p> <p>This work firstly demonstrates the importance of a COG2960 member for pathogen-host interaction, and suggests a common function conserved among members of this group.</p

Multicenter Collaborative Study of the Interaction of Antifungal Combinations against BrowZine Journal Cover Candida Spp. by Loewe Additivity and Bliss Independence-Based Response Surface Analysis

Combination antifungal therapy is widely used but not well understood. We analyzed the spectrophotometric readings from a multicenter study conducted by the New York State Department of Health to further characterize the in vitro interactions of the major classes of antifungal agents against Candida spp. Loewe additivity-based fractional inhibitory concentration index (FICi) analysis and Bliss independence-based response surface (BIRS) analysis were used to analyze two-drug inter- and intraclass combinations of triazoles (AZO) (voriconazole, posaconazole), echinocandins (ECH) (caspofungin, micafungin, anidulafungin), and a polyene (amphotericin B) against Candida albicans, C. parapsilosis, and C. glabrata. Although mean FIC indices did not differ statistically significantly from the additivity range of 0.5−4, indicating no significant pharmacodynamic interactions for all of the strain−combinations tested, BIRS analysis showed that significant pharmacodynamic interactions with the sum of percentages of interactions determined with this analysis were strongly associated with the FIC indices (Χ2 646, p \u3c 0.0001). Using a narrower additivity range of 1−2 FIC index analysis, statistically significant pharmacodynamic interactions were also found with FICi and were in agreement with those found with BIRS analysis. All ECH+AB combinations were found to be synergistic against all Candida strains except C. glabrata. For the AZO+AB combinations, synergy was found mostly with the POS+AB combination. All AZO+ECH combinations except POS+CAS were synergistic against all Candida strains although with variable magnitude; significant antagonism was found for the POS+MIF combination against C. albicans. The AZO+AZO combination was additive for all strains except for a C. parapsilosis strain for which antagonism was also observed. The ECH+ECH combinations were synergistic for all Candida strains except C. glabrata for which they were additive; no antagonism was found

Hsp90 governs dispersion and drug resistance of fungal biofilms

Fungal biofilms are a major cause of human mortality and are recalcitrant to most treatments due to intrinsic drug resistance. These complex communities of multiple cell types form on indwelling medical devices and their eradication often requires surgical removal of infected devices. Here we implicate the molecular chaperone Hsp90 as a key regulator of biofilm dispersion and drug resistance. We previously established that in the leading human fungal pathogen, Candida albicans, Hsp90 enables the emergence and maintenance of drug resistance in planktonic conditions by stabilizing the protein phosphatase calcineurin and MAPK Mkc1. Hsp90 also regulates temperature-dependent C. albicans morphogenesis through repression of cAMP-PKA signalling. Here we demonstrate that genetic depletion of Hsp90 reduced C. albicans biofilm growth and maturation in vitro and impaired dispersal of biofilm cells. Further, compromising Hsp90 function in vitro abrogated resistance of C. albicans biofilms to the most widely deployed class of antifungal drugs, the azoles. Depletion of Hsp90 led to reduction of calcineurin and Mkc1 in planktonic but not biofilm conditions, suggesting that Hsp90 regulates drug resistance through different mechanisms in these distinct cellular states. Reduction of Hsp90 levels led to a marked decrease in matrix glucan levels, providing a compelling mechanism through which Hsp90 might regulate biofilm azole resistance. Impairment of Hsp90 function genetically or pharmacologically transformed fluconazole from ineffectual to highly effective in eradicating biofilms in a rat venous catheter infection model. Finally, inhibition of Hsp90 reduced resistance of biofilms of the most lethal mould, Aspergillus fumigatus, to the newest class of antifungals to reach the clinic, the echinocandins. Thus, we establish a novel mechanism regulating biofilm drug resistance and dispersion and that targeting Hsp90 provides a much-needed strategy for improving clinical outcome in the treatment of biofilm infections