Acute ST-segment elevation myocardial infarction after amoxycillin-induced anaphylactic shock in a young adult with normal coronary arteries: a case report

BACKGROUND: Acute myocardial infarction (MI) following anaphylaxis is rare, especially in subjects with normal coronary arteries. The exact pathogenetic mechanism of MI in anaphylaxis remains unclear. CASE PRESENTATION: The case of a 32-year-old asthmatic male with systemic anaphylaxis, due to oral intake of 500 mg amoxycillin, complicated by acute ST-elevation MI is the subject of this report. Following admission to the local Health Center and almost simultaneously with the second dose of subcutaneous epinephrine (0.2 mg), the patient developed acute myocardial injury. Coronary arteriography, performed before discharge, showed no evidence of obstructive coronary artery disease. In vivo allergological evaluation disclosed strong sensitivity to amoxycillin and the minor (allergenic) determinants of penicillin. CONCLUSION: Acute ST-elevation MI is a rare but potential complication of anaphylactic reactions, even in young adults with normal coronary arteries. Coronary artery spasm appears to be the main causative mechanism of MI in the setting of "cardiac anaphylaxis". However, on top of the vasoactive reaction, a thrombotic occlusion, induced by mast cell-derived mediators and facilitated by prolonged hypotension, cannot be excluded as a possible contributory factor

New Insights in Plant Response to Viroid Infection by Differential Expression Analysis

Viroids are small circular RNA molecules, 246 to 400 nt long, which infect several crop plants and can cause diseases of economic importance. Citrus spp. are the hosts in which the highest number of viroids has been recovered. Citrus exocortis viroid (CEVd), causal agent of citrus exocortis disease, is responsible for several losses in citrus crops. Little is known about the molecular and cellular mechanisms by which viroids infect plants and produce symptoms. To deepen this knowledge, changes in the gene-expression profile during the early (pre–symptomatic) and the last (post-symptomatic) stage of Etrog citron infection by Citrus exocortis viroid (CEVd) were investigated using a citrus cDNA microarray. MaSigPro analysis was performed, and based on their expression profile along time, genes were divided into five clusters. These results will allow us to have a clearer idea about the changes in the plant transcriptome that are associated with symptom expression and/or with specific plant defense mechanisms

First generation of a mini-oligo array for rapid genome wide analysis of Plum pox virus

International audienc

Identification of transcription factors potencially involved in the juvenile to adult phase transition in Citrus

[EN] The juvenile to adult transition (JAT) in higher plants is required for them to reach reproductive competence. However, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. This work aims at increasing our understanding of the mechanisms regulating the JAT in citrus. Juvenile and adult plants from Pineapple sweet orange (Citrus sinensis) and Rough lemon (C. jambhiri) were used to screen for differentially expressed transcription factors (TFs) using a 115K microarray developed on the basis of the CitrusTF database. Murcott tangor (C. reticulata C. sinensis) and Duncan grapefruit (C. paradisi) were incorporated into the quantitative real-time reverse transcriptionPCR validation in order to select those genes whose phase-specific regulation was common to the four species. A browsable web database has been created with information about the structural and functional annotation related to 1152 unigenes of putative citrus TFs (CTFs). This database constitutes a valuable resource for research on transcriptional regulation and comparative genomics. Moreover, a microarray has been developed and used that contains these putative CTFs, in order to identify eight genes that showed differential expression in juvenile and adult meristems of four different species of citrus. Those genes have been characterized, and their expression pattern in vegetative and reproductive tissues has been analysed. Four of them are MADS-box genes, a family of TFs involved in developmental processes, whereas another one resembles MADS-box genes but lacks the MADS box itself. The other three showed high partial sequence similarity restricted to specific Arabidopsis protein domains but negligible outside those domains. The work presented here indicates that the JAT in citrus could be controlled by mechanisms that are in part common to those of Arabidopsis, but also somehow different, since specific factors without Arabidopsis orthologues have also been characterized. The potential involvement of the genes in the JAT is discussed.The Bioinformatics Core Service of the IBMCP (UPV-CSIC) is acknowledged for its help in bioinformatic analyses. This work was supported by Conselleria de Agricultura, Pesca y Alimentacion of Generalitat Valenciana [Proy_IVIA09/03] to G.A., and by the Ministry of 'Economia y Competividad'-Fondo Europeo de Desarrollo Regional (FEDER) [AGL2011-26490] and the Ministry of 'Ciencia e Innovacion' [AGL2008-01491] and Prometeo II 2013/008.Castillo, M.; Forment Millet, JJ.; Gadea Vacas, J.; Carrasco, J.; Juarez, J.; Navarro, L.; Ancillo, G. (2013). Identification of transcription factors potencially involved in the juvenile to adult phase transition in Citrus. 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