Classic Hodgkin lymphoma-nodular sclerosis – case report

Hodgkin's lymphoma is the third most common cancer in young people between 15 and 34 years of age, with a lower incidence in other age groups and slightly higher in males. The etiology of Hodgkin's lymphoma is still unknown - although various factors influencing the oncogenesis of the disease have been noted, the mechanism of their influence is poorly understood. This article will present the case of a 41-year-old patient with classic Hodgkin's lymphoma - nodular sclerosis histological subtype - chemorefractory

The clinical importance of PD‑L1 and PD‑1 expression in diffuse large B cell lymphoma

Introduction. The target pathway programmed cell death‑1 (PD‑1)/ programmed death‑ligand 1 (PD‑L1) shows clinical efficacy in solid tumors, but also in Hodgkin’s and non‑Hodgkin’s lymphoma. PD‑L1 expression most often correlates with a poor prognosis and a PD‑1 regulatory factor that mediates immunosuppression. The presence of an increased number of tumor‑infiltrating lymphocytes (TILs) PD‑1 + is a favorable prognostic factor in patients with diffuse large B cell lymphomas (DLBCLs) and follicular lymphomas, while the low number of TILs PD‑1 + is associated with an increased risk of histological transformation. In DLBCLs, TILs PD‑1 + may not reflect the depletion of T‑mediated tumor cells but may be an indicator of lymphoid cell origin. Objectives. The objective of this study was to describe the correlation between PD‑1 and PDL‑1 with survival in patients with the diagnosis of diffuse large B cell lymphoma. Material and methods. We have studied 80 patients and we have analyzed DLBCLs according to the Hans algorithm; in addition, we analyzed PD‑1 and PD‑L1 in tumor cells and in immune cells and we correlated this data with patient’s survival. Results. We found that there is a tendency of decreased survival and therapeutic response in DLBCL patients, with both an intense and weak PD‑L1 positivity in tumor cells. PD‑1 low positivity was associated in higher percentage with relapse and treatment unresponsiveness. Conclusions: Our data suggested that PD‑L1 expression correlates with a poor clinical response although it is not an independent prognostic marker and PD‑1 represents a favorable prediction factor for survival

Dynamics of the susceptibility to different antibiotics and chemical-therapy treatments of isolated germs from puerperal cow genital secretions

The study was conducted on a group of 10 cows, belonging to Bălţată cu Negru Românească breed, at the Cattle Research and Development Station of Dancu , Iaşi district. Genital secretions were sampled from puerperal cows (first 4 post-partum weeks), for determining the uterine bacterial flora and germ susceptibility to different antimicrobial products. The obtained results have shown a lower germ susceptibility in the first post-partum week, compared to the next weeks. In weeks 2,3 and 4 post-partum , the mean values of germ susceptibility to antibiotics had very high levels (90-100%) to Rifampine, Erythromycin, Cephalotin, average levels (70-83.3%) to Amoxicillin, Kanamycyn, Chloramphenicol, Streptomycin, Gentamicyn, bacteria showing resistance to Penicillin, Oxacicllin, Sulphametazol, Methycillin. The most frequent bacterium isolated from genital secretions of cows with inflammatory genital troubles was Arcanobacterium pyogenes, in single bacterial colony (31.6%) or in mixed bacterial colony with E.coli (5.3%)

Models for testing regenerative therapies – focus on explants as models for osteoarthritis

Background. Osteoarthritis (OA) is a degenerative disease that progressively involves all joint compartments leading to destruction and loss of function. Regenerative medicine (RM) aims to introduce revolutionary therapies dedicated to drastically improve the way we treat degenerative diseases including OA. Explanted cartilage tissue has been proposed as a modality to study cartilage ontogeny and to understand cartilage repair as well as modality to screen new drug/therapies for the treatment of OA. Objectives. To establish a working protocol for obtaining human osteochondral tissue explants in co -culture with synovial tissue to be used as ex vivo models for OA and to demonstrate explantreactivity to conditioned media (CM) from adipose mesenchymal cells (ADSC) tested as a modality for bone and cartilage rescue. Methods. Human osteochondral samples were collected from patients undergoing total knee replacement were kept in incomplete chondrogenic media (ICM) or in serum free DMEM.Explantreleased cytokines were quantified by. ELISA for Human tumor necrosis factor alpha(TNFα) and interleukin -6 (IL-6) and qPCR-based immunoassay for Human IL-17A and Human IL-1β (Proquantum™ immunoassay kit Invitrogen). Histology, Western blot and Immunohistochemistrystudies to detect Collagen type II (Col II) matrix metalloprotease 1 and 13 (MMP I, MMP-13),Perlecan and beta galactosidase (BGAL) are going on. Results. We found that culture media as well as synovial tissue presence influences the level ofdetectable IL-6, IL-17A CM increased IL-6 presence up to 29 days in culture; TNF α and IL1B levelsdecrease after 7 days in culture; CM treatment significantly decrease TNF α in both synoviumcontaining DMEM and ICM cultured explants Histology revealed presence of active chondrocytewith enlarged hyperchromatic nuclei in CM treated explants. Conclusion. Time in culture, type of culture media and synovial presence influence explant reactivity. Presence of synovial tissue increase explant reactivity especially for situation when an antiinflammatory effect is expected. Histology and immunohistochemistry can detect markers of tissue regeneration. Explant culture can serve as a reliable ex vivo model for testing both antiinflammatory as well as tissue remodeling intervention for articular joint repair

Changes in ecosystem services from wetland loss and restoration: An ecosystem assessment of the Danube Delta (1960–2010)

Deltaic flood plains provide critically important ecosystem services, including food production, fresh water, flood control, nutrient cycling, spiritual values and opportunities for recreation. Despite growing recognition of their societal and ecological importance, deltaic flood plains are declining worldwide at alarming rates. Loss of wetland ecosystem services bears socio-environmental costs overlooked in land-use planning. Conversely, wetland restoration can deliver important long-term benefits. This paper examines effects of different land use policies on ecosystem services provided by the Danube Delta, one of Europe’s largest and most outstanding wetlands. First, we identify, characterize and measure the most important ecosystem services provided by the Danube Delta. Second, we assess trends between 1960 and 2010, contrasting periods of economic development (1960–1989) and ecological restoration (1990–2010). Our results indicate that i) the Danube Delta provides important services with benefits accrue from local communities to humanity at large, ii) that two thirds of the Delta’s ecosystem services have declined over the studied period and iii) that ongoing restoration efforts have so far been unable to reverse trends in ecosystem service decline. Benefits from ecological restoration policies are already becoming apparent, but at a scale not yet comparable to the costs from ecosystem decline incurred over previous decades. Ecosystem assessment Socio-environmental costs Wetlands Restoration Danube Delta RomaniaacceptedVersio

Magnetic Nanoemulsions for the Intra-Articular Delivery of Ascorbic Acid and Dexamethasone

(1) Osteoarthritis (OA) is a progressive joint degenerative disease that currently has no cure. Limitations in the development of innovative disease modifying therapies are related to the complexity of the underlying pathogenic mechanisms. In addition, there is the unmet need for efficient drug delivery methods. Magnetic nanoparticles (MNPs) have been proposed as an efficient modality for the delivery of bioactive molecules within OA joints, limiting the side effects associated with systemic delivery. We previously demonstrated MNP’s role in increasing cell proliferation and chondrogenesis. In the design of intra-articular therapies for OA, the combined NE-MNP delivery system could provide increased stability and biological effect. (2) Proprietary Fe3O4 MNPs formulated as oil-in-water (O/W) magneto nanoemulsions (MNEs) containing ascorbic acid and dexamethasone were tested for size, stability, magnetic properties, and in vitro biocompatibility with human primary adipose mesenchymal cells (ADSC), cell mobility, and chondrogenesis. In vivo biocompatibility was tested after systemic administration in mice. (3) We report high MNE colloidal stability, magnetic properties, and excellent in vitro and in vivo biocompatibility. By increasing ADSC migration potential and chondrogenesis, MNE carrying dexamethasone and ascorbic acid could reduce OA symptoms while protecting the cartilage layer

Research and Science Today No.2

Research and Science Today Journal is a publication founded in 2011 and it is dedicated to the students of all levels (license, master and doctoral) of faculties in the country and abroad. We want to offer the participants the opportunity to present their scientific works in the following areas: Social Sciences, Economic Sciences, Legal Sciences, Humanities, Education Sciences, Engineering, Medicine and Sport. This journal provides students the opportunity to create and / or to improve their abilities to write scientific papers. So each appearance (two appearances per year at which we can add supplements) contains a number of papers written by students, masters and doctoral from the faculties from the country or / and abroad. The journal promotes original studies contributing to the progress of knowledge and it is motivated by the need to address issues of theory and practice in the areas mentioned above. The Journal is a training means of the factors involved in the conceptualization, development, implementation and evaluation , aiming the formation of creative personalities who could be able to adapt through the changing conditions of life. Journal wants to be a forum for debates disciplinaries and interdisciplinaries theoretical topics, to become a research support, to leverage this work at regional, national and international levels. We believe that this gathering will enjoy the support from both parts of the researchers and of the practitioners, and will provide appropriate training sources held professional through the current problems

Research and Science Today No.2

Research and Science Today Journal is a publication founded in 2011 and it is dedicated to the students of all levels (license, master and doctoral) of faculties in the country and abroad. We want to offer the participants the opportunity to present their scientific works in the following areas: Social Sciences, Economic Sciences, Legal Sciences, Humanities, Education Sciences, Engineering, Medicine and Sport. This journal provides students the opportunity to create and / or to improve their abilities to write scientific papers. So each appearance (two appearances per year at which we can add supplements) contains a number of papers written by students, masters and doctoral from the faculties from the country or / and abroad. The journal promotes original studies contributing to the progress of knowledge and it is motivated by the need to address issues of theory and practice in the areas mentioned above. The Journal is a training means of the factors involved in the conceptualization, development, implementation and evaluation , aiming the formation of creative personalities who could be able to adapt through the changing conditions of life. Journal wants to be a forum for debates disciplinaries and interdisciplinaries theoretical topics, to become a research support, to leverage this work at regional, national and international levels. We believe that this gathering will enjoy the support from both parts of the researchers and of the practitioners, and will provide appropriate training sources held professional through the current problems

IMPLICAREA RENALĂ ÎN SEPSISUL SEVER ŞI ÎN ŞOCUL SEPTIC LA SUGAR ŞI COPILUL MIC

Deşi mortalitatea prin sepsis a scăzut în ultimii ani, şocul septic rămâne o cauză majoră de morbiditate şi mortalitate la copilul sub vârsta de cinci ani. Rinichiul este implicat şi constant afectat în această patologie, infecţiile bacteriene reprezentând o cauză frecventă de îmbolnăvire. Insufi cienţa renală acută poate fi principala manifestare a bolii şi o problemă terapeutică majoră. Autorii prezintă implicarea renală în meningococemie şi în sindromul hemolitic uremic cauzat de E coli O157:H7 şi de pneumococ, boli ce necesită abordare diagnostică şi terapeutică specifi că