25 research outputs found
One Coin, No Need to Flip: Shared PET Targets in Cancer and Coronary Artery Disease
OBJECTIVE. The purposes of this article are to review the common
biologic features of cancer and coronary artery disease assessed with
PET tracers, focusing on those already used in the clinic and those with
translational potential, and to discuss the current value and expected
contribution of PET in diagnosis, risk stratification, and treatment
monitoring.
CONCLUSION. PET using a wide variety of radiotracers enhances
understanding of pathophysiologic changes shared by cancer and coronary
artery disease, helps establish an accurate diagnosis, and aids in
prognostic assessment and management decisions. It is likely that with
the evolution of therapeutic strategies for blocking the development and
progression of both diseases and with the introduction of novel,
specific ligands in clinical practice, PET will play an ever stronger
role in diagnosis, risk stratification, and monitoring of therapy
Removal of mercury from aqueous solutions by malt spent rootlets
Summarization: Mercury poses a severe threat to environment due to its toxicity, even at low concentrations. Biosorption
is a promising, low cost, and environmentally friendly clean up technique. Malt spent rootlets (MSR), a
brewery by-product, were used as sorbents for the removal of mercury from aquatic systems. The effect
of the solution pH, contact time between sorbent, solid to liquid ratio, and initial mercury concentration
on mercury removal were investigated experimentally. It was found that the optimum pH for the mercury
sorption onto MSR was approximately 5. Sorption kinetic experiments revealed that mercury sorption
is a relatively rapid process, where film diffusion and intra-particle diffusion play an important role.
The kinetic data were successfully described by both the pseudo-second-order and Elovich models. The
isotherm data were adequately fitted by the Langmuir model determining a monolayer capacity qmax
equal to 50 mg/g and suggesting a functional group-limited sorption process. MSR were capable of
removing significant amounts of mercury, mainly due to the carboxyl and phosphonate groups of their
surfaces.Παρουσιάστηκε στο: The Chemical Engineering Journa
Innovative multimodal DOTA/NODA nanoparticles for MRI and PET imaging for tumor detection
International audienc
Tumor Targeting via Sialic Acid: [<sup>68</sup>Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET
Purpose: The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia). Procedures: The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en-pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent. Results: The affinity of [68Ga]DOTA-en-pba to Sia was demonstrated on B16-F10 melanoma cells, after the production of melanin as well as Sia overexpression was proved to be up to four times higher in this cell line compared to that in Mel-C cells. Biodistribution studies in B16-F10 tumor-bearing SCID mice showed blood clearance at the time points studied, while uptake in the tumor peaked at 60 min post-injection (6.36 ± 2.41 % ID/g). The acquired PET images were in accordance with the ex vivo biodistribution results. Metabolite assessment on tumor, blood, and urine samples showed that [68Ga]DOTA-en-pba remains unmetabolized up to at least 60 min post-injection. Conclusions: Our work is the first attempt for in vivo imaging of cancer by targeting overexpression of sialic acid on cancer cells with a radiotracer in PET.BT/Biocatalysi
Neutrophil extracellular traps in giant cell arteritis biopsies: presentation, localization and co-expression with inflammatory cytokines
Objectives To explore the presence of neutrophil extracellular traps
(NETs) in inflamed temporal artery biopsies (TABs) of patients with GCA.
Methods Ten patients with GCA [five with limited and five with
associated generalized vascular involvement, as defined by
F-18-fluorodeoxyglucose PET with CT (PET/CT)] and eight with PMR were
studied. The presence, location, quantitation and decoration of NETs
with IL-6, IL-1 beta and IL-17A were assessed in TABs at the time of
disease diagnosis by tissue immunofluorescence and confocal microscopy.
Paired serum levels of IL-6 and IL-17A were also evaluated in all
patients. Results All temporal artery biopsies from GCA, but not PMR,
patients had NETs located mainly in the adventitia, adjacent to the vasa
vasorum. NETs decorated with IL-6 were present in 8/10 TABs of GCA
patients, of whom 5 were PET/CT(+) and 3 PET/CT(-) patients. IL-17A(+)
NETs were observed in all GCA patients. IL-1 beta(+) NETs were not
detected in any GCA patient. No relation was found between serum IL-6
and IL-17A levels and NETs containing IL-6 and/or IL-17A. Conclusions
NETs bearing pro-inflammatory cytokines are present in inflamed
GCA-TABs. Future studies with a larger number of patients from different
centres will show whether the findings regarding neutrophils/NETs in the
TAB are consistent and disclose their clinical impact